Foreword
The use of chemical pesticides in agriculture raises the concern about their hazardous effects
on the environment. There is a need for a change in crop protection methods that are safe to
humans and environment.
Entomopathogenic nematodes with their symbiotic bacteria play an important role in
the field of insect pest management. As these nematodes are eco-friendly, they are used
worldwide as biocontrol agents as an effective alternative solution to chemical pesticides.
Entomopathogenic nematodes are currently receiving most attention for their bio-efficacy.
This book is a complete systematic approach to various entomopathogenic nematode
procedures which help in identifying and characterizing them for agricultural pest
management.
The commitment and the efforts put forth by the authors Dr. S. Sivaramakrishnan,
Professor and Head, Department of Biotechnology, Bharathidasan University, Tiruchirappalli, and Dr. M. Razia, Assistant Professor, Department of Biotechnology, Mother Teresa
Women’s University, Kodaikanal, in designing this manual is highly appreciated and welcomed. Thoughtfully planned and expertise care is taken to pool all the relevant features of
entomopathogenic nematodes and their symbiotic bacteria in a single manual in a wellorganized manner, signifying the uniqueness of this manual. The contents are lucid and
noteworthy. The protocols included are clearly defined and stepwise. This book will surely
meet the laboratory challenges and will be a valuable resource to the readers.
This laboratory manual is an excellent contribution of the authors to the discipline of
nematology and will definitely do good to the hands of bio-application.
Bharathidasan University, Tiruchirappalli,
Tamil Nadu, India
P. Manisankar
v
The use of chemical pesticides in agriculture raises the concern about their hazardous effects
on the environment. There is a need for a change in crop protection methods that are safe to
humans and environment.
Entomopathogenic nematodes with their symbiotic bacteria play an important role in
the field of insect pest management. As these nematodes are eco-friendly, they are used
worldwide as biocontrol agents as an effective alternative solution to chemical pesticides.
Entomopathogenic nematodes are currently receiving most attention for their bio-efficacy.
This book is a complete systematic approach to various entomopathogenic nematode
procedures which help in identifying and characterizing them for agricultural pest
management.
The commitment and the efforts put forth by the authors Dr. S. Sivaramakrishnan,
Professor and Head, Department of Biotechnology, Bharathidasan University, Tiruchirappalli, and Dr. M. Razia, Assistant Professor, Department of Biotechnology, Mother Teresa
Women’s University, Kodaikanal, in designing this manual is highly appreciated and welcomed. Thoughtfully planned and expertise care is taken to pool all the relevant features of
entomopathogenic nematodes and their symbiotic bacteria in a single manual in a wellorganized manner, signifying the uniqueness of this manual. The contents are lucid and
noteworthy. The protocols included are clearly defined and stepwise. This book will surely
meet the laboratory challenges and will be a valuable resource to the readers.
This laboratory manual is an excellent contribution of the authors to the discipline of
nematology and will definitely do good to the hands of bio-application.
Bharathidasan University, Tiruchirappalli,
Tamil Nadu, India
P. Manisankar
v