5. Introduce other odors (insect headspace or odorants) using a
10-ml Luer-Lok Becton Dickinson syringe with a Becton Dickinson needle (21 G 1.5
00 ) with the end clipped to be blunt.
5.4 Host Chemotaxis
Assay [156]
5.4.1 Materials Required
l
Nematodes.
l
Nalgene (8050-0250) FTP 3/16
00 ID tubing.
l
Gastight syringe (50 ml).
l
Soda lime pellets.
l
Teflon tape.
5.4.2 Procedure
1. Host headspace is established with introducing several hosts
inside a 50 ml gastight syringe, depending on host size the
number will fluctuate.
2. Host headspace attraction can also be determined without the
aid of host-induced CO 2 by introducing a column of soda lime
into the test setup.
3. Fill a 6-in. column of Nalgene (8050-0250) FTP 3/16
00 ID
tubing with 2–5 mm soda lime pellets.
4. Fit the column on one end with fitting 731-8226, which can
fasten to the tubing which runs to the assay plate lid, whereas to
the other end of the column is attached to fitting 731-8223
from a low-pressure fitting kit.
5. Fit tightly the last part of the column onto the Luer-Lok end of
the 50-ml syringe.
6. Wrap the fittings in Teflon tape for airtight fit.
7. Count finally the number of worms in the scoring regions on
either side of the plate.
8. Calculate chemotaxis index.
5.5 Nematode
Chemotaxis Using
Microfluidics [157]
This assay is a simple method to determine the nematode chemotaxis with regard to numerous chemicals.
5.5.1 Materials Required
l
Craft cutter.
l
Nematodes.
l
Fresh wax worms.
l
Mortar and pestle.
l
70% isopropanol.
l
5% acetic acid (white vinegar).
l
Agar powder.
l
Transparency film.
l
Double-sided adhesive.
Chemotaxis Assay
43
10-ml Luer-Lok Becton Dickinson syringe with a Becton Dickinson needle (21 G 1.5
00 ) with the end clipped to be blunt.
5.4 Host Chemotaxis
Assay [156]
5.4.1 Materials Required
l
Nematodes.
l
Nalgene (8050-0250) FTP 3/16
00 ID tubing.
l
Gastight syringe (50 ml).
l
Soda lime pellets.
l
Teflon tape.
5.4.2 Procedure
1. Host headspace is established with introducing several hosts
inside a 50 ml gastight syringe, depending on host size the
number will fluctuate.
2. Host headspace attraction can also be determined without the
aid of host-induced CO 2 by introducing a column of soda lime
into the test setup.
3. Fill a 6-in. column of Nalgene (8050-0250) FTP 3/16
00 ID
tubing with 2–5 mm soda lime pellets.
4. Fit the column on one end with fitting 731-8226, which can
fasten to the tubing which runs to the assay plate lid, whereas to
the other end of the column is attached to fitting 731-8223
from a low-pressure fitting kit.
5. Fit tightly the last part of the column onto the Luer-Lok end of
the 50-ml syringe.
6. Wrap the fittings in Teflon tape for airtight fit.
7. Count finally the number of worms in the scoring regions on
either side of the plate.
8. Calculate chemotaxis index.
5.5 Nematode
Chemotaxis Using
Microfluidics [157]
This assay is a simple method to determine the nematode chemotaxis with regard to numerous chemicals.
5.5.1 Materials Required
l
Craft cutter.
l
Nematodes.
l
Fresh wax worms.
l
Mortar and pestle.
l
70% isopropanol.
l
5% acetic acid (white vinegar).
l
Agar powder.
l
Transparency film.
l
Double-sided adhesive.
Chemotaxis Assay
43