5.3 Jumping Assay
[156]
5.3.1 Materials Required
l
Nematodes.
l
Tap water.
l
Odorants.
l
CO 2 .
l
Petri dish.
l
Whatman 1 filter paper.
l
Hamilton gastight syringe.
l
Luer-Lok Becton Dickinson syringe.
5.3.2 Procedure
1. Drill a 1.25-mm hole from side to side of a 5-cm petri dish and
lid to permit odors to be added with the lid on, which prevents
drying or the confounding effects of drafts.
2. Keep a 55-mm Whatman 1 filter paper on the underside of the
dish to attract and hold moisture, thus supplying a fibrous
material to make jumping easy. The filter paper performs as a
soil-like layer.
3. Apply to this ~200 μl of tap water suspended with <100
nematodes.
4. Introduce gases (10% CO 2 ) through a 10-ml Hamilton gastight syringe fitted by a Hamilton blunt needle (22s/2
00 /3).
Fig. 2 Template used to score chemotaxis assays for chemotaxis index
42
Chemical Ecology
[156]
5.3.1 Materials Required
l
Nematodes.
l
Tap water.
l
Odorants.
l
CO 2 .
l
Petri dish.
l
Whatman 1 filter paper.
l
Hamilton gastight syringe.
l
Luer-Lok Becton Dickinson syringe.
5.3.2 Procedure
1. Drill a 1.25-mm hole from side to side of a 5-cm petri dish and
lid to permit odors to be added with the lid on, which prevents
drying or the confounding effects of drafts.
2. Keep a 55-mm Whatman 1 filter paper on the underside of the
dish to attract and hold moisture, thus supplying a fibrous
material to make jumping easy. The filter paper performs as a
soil-like layer.
3. Apply to this ~200 μl of tap water suspended with <100
nematodes.
4. Introduce gases (10% CO 2 ) through a 10-ml Hamilton gastight syringe fitted by a Hamilton blunt needle (22s/2
00 /3).
Fig. 2 Template used to score chemotaxis assays for chemotaxis index
42
Chemical Ecology