interactions are disrupted with SDS, phenol, or proteinase
K. Proteinase enzyme is used to degrade the proteins in the disrupted cell soup. Phenol and chloroform are involved to denature
and separate individual proteins from DNA. Chloroform is also a
protein denaturant, which stabilizes the somewhat unstable boundary between an aqueous phase and pure phenol layer. The denatured proteins form a layer at the interface between the aqueous
and the organic phases which are removed by centrifugation. DNA
Fig. 2 API 50 CH system (biochemical test for the identification of symbiotic bacteria)
132
Laboratory Techniques for Symbiotic Bacteria
K. Proteinase enzyme is used to degrade the proteins in the disrupted cell soup. Phenol and chloroform are involved to denature
and separate individual proteins from DNA. Chloroform is also a
protein denaturant, which stabilizes the somewhat unstable boundary between an aqueous phase and pure phenol layer. The denatured proteins form a layer at the interface between the aqueous
and the organic phases which are removed by centrifugation. DNA
Fig. 2 API 50 CH system (biochemical test for the identification of symbiotic bacteria)
132
Laboratory Techniques for Symbiotic Bacteria