into a larger petri dish (150 Â 25 mm), containing sterile
distilled water into which the IJs can migrate.
9. Harvest the IJs in the larger petri dish from the nematode traps
every alternate day for about 3 weeks and wash three times in
distilled water.
10. Count from each petri dish and store at 20
C (Fig. 16).
9 Methods of Bioassay
The virulence and bioefficacy of entomopathogenic nematodes are
evaluated against larval stages of insect species belonging to order
Lepidoptera.
Fig. 16 Mass production of entomopathogenic nematodes (in solid medium)
108
Laboratory Techniques for Entomopathogenic Nematodes
distilled water into which the IJs can migrate.
9. Harvest the IJs in the larger petri dish from the nematode traps
every alternate day for about 3 weeks and wash three times in
distilled water.
10. Count from each petri dish and store at 20
C (Fig. 16).
9 Methods of Bioassay
The virulence and bioefficacy of entomopathogenic nematodes are
evaluated against larval stages of insect species belonging to order
Lepidoptera.
Fig. 16 Mass production of entomopathogenic nematodes (in solid medium)
108
Laboratory Techniques for Entomopathogenic Nematodes