11.19 Future Outlook: The Challenges In Cellulase Research
Hidden aspects related to the efficient development of the biocatalyst and advancement of technical knowledge for yielding and employment of cellulase brought into
light by the efforts of numerous explorative works. No solitary methodologies stood
cheaper and efficient in the transformation of the native cellulosic material for the
generation of worthy target outputs or biofuel. In the present trend, biotransformation of lignocellulosic waste by the use of cellulase build-ups available from the
market is not economically advantageous.
The following are prime objectives for forthcoming investigations on cellulose:
1. Upgrading the activity of cellulase for greater potential of decreasing the concentration of the desired enzyme (Qin et al. 2004).
2. Trimming cellulase production cost.
3. Economic feasibility of cellulase production by guarded genetic modifications
into the metabolisms of cellulose degraders for upgraded productions.
4. Negating the feedback loops by glucose and achievements in cohesive bioprocess
for the production of cellulases.
5. More fundamental research is required to produce designer enzymes good
enough for clear-cut applications.
6. Extended studies on enhancement of cellulase action or conferring of required
attributes to enzymes via manipulation of protein structures.
The aspects regarding the bioconversion of cellulose materials become the
bottom line of forthcoming researches associated with cellulase and cellulose
degraders. The problem which raises the attention is not restricted to enzyme
production alone but a concerted effort to figure out the basic physiology of
cellulolytic microbes, and the utilisation of this knowledge merged with the
engineering principles to achieve greater heights in processing and exploitation
of the most copious innate reserves. The aspects disclosed to consideration
include pretreatment methods of cellulosic materials for an easy microbial access,
cost-effective approaches for production of hydrolytic enzyme products, organism development of metabolic engineering and eventually protein engineering to
improve the characteristics of enzymes to increase their specific activities, process
tolerance and stability (Sukumaran et al. 2005).
11.20 Conclusion
Among the various commercially available cellulases, fungal cellulases gain a
cutting-edge advantage owing to pronounced yield, efficiency, effortless
crystallisation and a higher degree of purity. In the development of any bioprocess
technology, choosing efficient, modest, and handy raw materials for the production
of biocatalyst besides optimisation of media components and fermentation
11 Significance of Process Parameters on Fungal Cellulase Production
315
Hidden aspects related to the efficient development of the biocatalyst and advancement of technical knowledge for yielding and employment of cellulase brought into
light by the efforts of numerous explorative works. No solitary methodologies stood
cheaper and efficient in the transformation of the native cellulosic material for the
generation of worthy target outputs or biofuel. In the present trend, biotransformation of lignocellulosic waste by the use of cellulase build-ups available from the
market is not economically advantageous.
The following are prime objectives for forthcoming investigations on cellulose:
1. Upgrading the activity of cellulase for greater potential of decreasing the concentration of the desired enzyme (Qin et al. 2004).
2. Trimming cellulase production cost.
3. Economic feasibility of cellulase production by guarded genetic modifications
into the metabolisms of cellulose degraders for upgraded productions.
4. Negating the feedback loops by glucose and achievements in cohesive bioprocess
for the production of cellulases.
5. More fundamental research is required to produce designer enzymes good
enough for clear-cut applications.
6. Extended studies on enhancement of cellulase action or conferring of required
attributes to enzymes via manipulation of protein structures.
The aspects regarding the bioconversion of cellulose materials become the
bottom line of forthcoming researches associated with cellulase and cellulose
degraders. The problem which raises the attention is not restricted to enzyme
production alone but a concerted effort to figure out the basic physiology of
cellulolytic microbes, and the utilisation of this knowledge merged with the
engineering principles to achieve greater heights in processing and exploitation
of the most copious innate reserves. The aspects disclosed to consideration
include pretreatment methods of cellulosic materials for an easy microbial access,
cost-effective approaches for production of hydrolytic enzyme products, organism development of metabolic engineering and eventually protein engineering to
improve the characteristics of enzymes to increase their specific activities, process
tolerance and stability (Sukumaran et al. 2005).
11.20 Conclusion
Among the various commercially available cellulases, fungal cellulases gain a
cutting-edge advantage owing to pronounced yield, efficiency, effortless
crystallisation and a higher degree of purity. In the development of any bioprocess
technology, choosing efficient, modest, and handy raw materials for the production
of biocatalyst besides optimisation of media components and fermentation
11 Significance of Process Parameters on Fungal Cellulase Production
315