10.11 Pharmaceutical Industry
Cellulases are becoming popular biocatalysts in pharmaceutical industry as they are
able to release therapeutic compounds from plant-based biomass. Crude cellulases
have shown increased ellagic acid yield through hydrolysis from ellagitannins
(Do et al. 2009). Researches have shown the enhanced effect on the extraction of
the biologically active polyphenols using crude cellulases from various cereals
(Chen et al. 2005; Al-Ghazzewi et al. 2007). Crude cellulose increases the antioxidant properties of wheat by increasing the polyphenol extraction through free radical
scavenging using enzymatic hydrolysis (Chen et al. 2005). Cellulases as
supplements are gaining acceptance more and more due to the inability of digesting
cellulose by human beings. As such, various digestive aids using cellulases (such as
P-A-L Plus Enzymes, Digestion, etc.) are manufactured for the treatment of metabolic disorders of people (Karmakar and Ray 2011).
Prebiotics contain nondigestible fibers that help the growth of beneficial bacteria
for the large intestine passing through the upper gastrointestinal tract.
Glucomannans, a water-soluble polysaccharide, act as potent prebiotics when
depolymerized using cellulases and mannanases which boost the prebiotic effect
as well (Albrecht et al. 2009; Connolly et al. 2010).
The mixture of Konjac glucomannan (KGM) oligosaccharide, a prebiotic, was
prepared using endo-β-(1,4)-glucanase and endo-β-(1,4)-mannanase from KGM
polysaccharide. In vitro fermentation was observed with gut flora of human for the
change in structure of the mixture of these KGM oligosaccharides for about 72 hours
in the presence of matrix-assisted laser desorption/ionization-time-of-flight mass
spectrometry (MALDI-TOF MS) and capillary electrophoresis-laser-induced fluorescence detection (CE-LIF). As per the observation, KGM oligosaccharide showed
higher degradability produced by endo-β-(1,4)-glucanase compared to endo-β-(1,4)mannanase. Desirable bacteria like Lactobacilli and Bifidobacterium increase in
Fig. 10.5 Ethanol production showing the enzymes produced at different stages
10 Efficiency Analysis of Crude Versus Pure Cellulase in Industry
293
Cellulases are becoming popular biocatalysts in pharmaceutical industry as they are
able to release therapeutic compounds from plant-based biomass. Crude cellulases
have shown increased ellagic acid yield through hydrolysis from ellagitannins
(Do et al. 2009). Researches have shown the enhanced effect on the extraction of
the biologically active polyphenols using crude cellulases from various cereals
(Chen et al. 2005; Al-Ghazzewi et al. 2007). Crude cellulose increases the antioxidant properties of wheat by increasing the polyphenol extraction through free radical
scavenging using enzymatic hydrolysis (Chen et al. 2005). Cellulases as
supplements are gaining acceptance more and more due to the inability of digesting
cellulose by human beings. As such, various digestive aids using cellulases (such as
P-A-L Plus Enzymes, Digestion, etc.) are manufactured for the treatment of metabolic disorders of people (Karmakar and Ray 2011).
Prebiotics contain nondigestible fibers that help the growth of beneficial bacteria
for the large intestine passing through the upper gastrointestinal tract.
Glucomannans, a water-soluble polysaccharide, act as potent prebiotics when
depolymerized using cellulases and mannanases which boost the prebiotic effect
as well (Albrecht et al. 2009; Connolly et al. 2010).
The mixture of Konjac glucomannan (KGM) oligosaccharide, a prebiotic, was
prepared using endo-β-(1,4)-glucanase and endo-β-(1,4)-mannanase from KGM
polysaccharide. In vitro fermentation was observed with gut flora of human for the
change in structure of the mixture of these KGM oligosaccharides for about 72 hours
in the presence of matrix-assisted laser desorption/ionization-time-of-flight mass
spectrometry (MALDI-TOF MS) and capillary electrophoresis-laser-induced fluorescence detection (CE-LIF). As per the observation, KGM oligosaccharide showed
higher degradability produced by endo-β-(1,4)-glucanase compared to endo-β-(1,4)mannanase. Desirable bacteria like Lactobacilli and Bifidobacterium increase in
Fig. 10.5 Ethanol production showing the enzymes produced at different stages
10 Efficiency Analysis of Crude Versus Pure Cellulase in Industry
293