Table 7.3
Ethanol generation from different 1G and 2G feedstock using different pretreatment and fermentation method
Feedstock
Carbohydrate content
Pretreatment and
hydrolysis (liquefaction
and saccharification)
Fermentation condition
Ethanol yield
References
Corn varieties high
sugary corn genotypes
(HSG) parent
field
corn lines (PFCs)
HSG: starch 67.3%,
total sugar 7.4%;
PFC-starch 73.6%, total
sugar 1.2%
Saccharification by
STARGEN 002; enzyme
load 2 Kg/MT; initial
solid concentration
300 g/L
S. cerevisiae ATCC
96581
(2 mL inoculums /100mL
media) incubated at 30
C,
4.2 pH for 96 h
Ethanol yield: HSG:
17.94%, 141.5 g/L; PFC:
16.85%, 130.5 g/L
Zabed et al.
(2016b)
Cassava
flour
Starch 76%–81%
High temperature
liquefaction using
Spezyme, amylase, and
glucoamylase at 80
C for
90 min
S. cerevisiae
(inoculum
1.5
 10
7
cells/mL)
incubated at 30
C, 5.5 pH
for 72 h
Ethanol yield: At lab
scale: 86.1% (17.2% v/v)
theoretical yield. At pilot
scale: 83.6% (16.5% v/v)
of the theoretical yield
Shanavas
et al. (2011);
Nguyen
et al. (2014)
Wild cassava
Carbohydrate content:
32–35% (fresh weight)
80–90% (dry matter)
Liquefaction using
α-amylase and
saccharification and
β-glucanase
Caloramator boliviensis
(inoculum load
50 mL/240 ml media)
incubated at 60
C, 7 pH
for 48 h
Ethanol yield: 33.0 g/L,
85% of the theoretical
ethanol yield
Montagnac
et al. (2009);
Moshi et al.
(2015)
Sorghum juice
Not available
Not available
S. cerevisiae
strain
BY4741 (inoculum load
5
 10
8
cells∙mL
À1
)
incubated at 30
C, pH 5.2
for 48 h
Ethanol yield: 115.2 g/L,
87.1% theoretical yield
(Sasaki et al.
(2015))
Sugarcane molasses
Sucrose: 31%; Inverted
sugar: 15%
Not available
Aule alcohol yeast
(AY) and Aule baker’s
yeast (BY) (inoculum 1%
w/v) incubated at RT, 4.3
pH for 72 h
Ethanol yield: AY-74.8
g/L, BY-102.9 g/L from
300 g/L sugar
concentration
Mayzuhroh
et al. (2016)
(continued)
7 Bioethanol Production: Generation-Based Comparative Status Measurements
179
Ethanol generation from different 1G and 2G feedstock using different pretreatment and fermentation method
Feedstock
Carbohydrate content
Pretreatment and
hydrolysis (liquefaction
and saccharification)
Fermentation condition
Ethanol yield
References
Corn varieties high
sugary corn genotypes
(HSG) parent
field
corn lines (PFCs)
HSG: starch 67.3%,
total sugar 7.4%;
PFC-starch 73.6%, total
sugar 1.2%
Saccharification by
STARGEN 002; enzyme
load 2 Kg/MT; initial
solid concentration
300 g/L
S. cerevisiae ATCC
96581
(2 mL inoculums /100mL
media) incubated at 30
C,
4.2 pH for 96 h
Ethanol yield: HSG:
17.94%, 141.5 g/L; PFC:
16.85%, 130.5 g/L
Zabed et al.
(2016b)
Cassava
flour
Starch 76%–81%
High temperature
liquefaction using
Spezyme, amylase, and
glucoamylase at 80
C for
90 min
S. cerevisiae
(inoculum
1.5
 10
7
cells/mL)
incubated at 30
C, 5.5 pH
for 72 h
Ethanol yield: At lab
scale: 86.1% (17.2% v/v)
theoretical yield. At pilot
scale: 83.6% (16.5% v/v)
of the theoretical yield
Shanavas
et al. (2011);
Nguyen
et al. (2014)
Wild cassava
Carbohydrate content:
32–35% (fresh weight)
80–90% (dry matter)
Liquefaction using
α-amylase and
saccharification and
β-glucanase
Caloramator boliviensis
(inoculum load
50 mL/240 ml media)
incubated at 60
C, 7 pH
for 48 h
Ethanol yield: 33.0 g/L,
85% of the theoretical
ethanol yield
Montagnac
et al. (2009);
Moshi et al.
(2015)
Sorghum juice
Not available
Not available
S. cerevisiae
strain
BY4741 (inoculum load
5
 10
8
cells∙mL
À1
)
incubated at 30
C, pH 5.2
for 48 h
Ethanol yield: 115.2 g/L,
87.1% theoretical yield
(Sasaki et al.
(2015))
Sugarcane molasses
Sucrose: 31%; Inverted
sugar: 15%
Not available
Aule alcohol yeast
(AY) and Aule baker’s
yeast (BY) (inoculum 1%
w/v) incubated at RT, 4.3
pH for 72 h
Ethanol yield: AY-74.8
g/L, BY-102.9 g/L from
300 g/L sugar
concentration
Mayzuhroh
et al. (2016)
(continued)
7 Bioethanol Production: Generation-Based Comparative Status Measurements
179