Fig. 6 Schematic representation of multiplex ICA. a The NC membrane, absorbent pad, and
sample pad were assembled sequentially onto a plastic backing sheet. The NC membrane was
modified with the goat anti-mouse IgG antibody and five kinds of antigens to form one control line
and five test lines, respectively. b In absence of any analyte, mAb–AuNP conjugates would bind to
the five test lines and the control line, forming red bands at all the lines. However, in presence of
the analytes, the mAb–AuNP conjugates would bind to the corresponding free analytes and
analyte–mAbs–AuNP complexes would be formed. Those complexes would not bind to the
hapten–protein conjugates or aggregate on the five test lines and thus no red bands would be
formed when they passed through the test lines. However, they reacted with the goat-mouse IgG
antibody at the control line, and gathered there forming deep red bands (reproduced with permission from [33], copyright 2015, Elsevier
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sample pad were assembled sequentially onto a plastic backing sheet. The NC membrane was
modified with the goat anti-mouse IgG antibody and five kinds of antigens to form one control line
and five test lines, respectively. b In absence of any analyte, mAb–AuNP conjugates would bind to
the five test lines and the control line, forming red bands at all the lines. However, in presence of
the analytes, the mAb–AuNP conjugates would bind to the corresponding free analytes and
analyte–mAbs–AuNP complexes would be formed. Those complexes would not bind to the
hapten–protein conjugates or aggregate on the five test lines and thus no red bands would be
formed when they passed through the test lines. However, they reacted with the goat-mouse IgG
antibody at the control line, and gathered there forming deep red bands (reproduced with permission from [33], copyright 2015, Elsevier
Immunochromatographic Strip Based Sensor for the Detection …
241