20
Glycerol biotechnological production in CO 2 -fixing microorganisms has been
reported in several studies. For instance, Chow et al. (2013) highlighted the exploitability of the microalga Dunaliella tertiolecta as a natural glycerol producer based
on the observation that extracellular glycerol release acts as a valuable carbon sink
for photosynthetic carbon dioxide capture. The peak concentration amounted to
5.5 g/L upon an 80-day-long cultivation in non-optimized light conditions. In
Savakis et al. (2015), a Synechocystis sp. PCC 6803, engineered by the heterologous
expression of the phosphoglycerol phosphatase 2 encoded by the gpp2 gene from
Saccharomyces cerevisiae, accumulated a glycerol extracellular concentration of
14.3 mM after a 17-day-long cultivation under salt stress condition.
2,3-Butanediol
The chemical compound 2,3-butanediol (2,3-BDO) is gaining share in the global market as an intermediate compound for manufacturing plastics. 2,3-BDO can be transformed with 94% selectivity into 1,3-butadien (Duan et al. 2015), which is a precursor
for a variety of polymer and copolymer materials. The esterification of several dicarboxylic acids with 2,3-BDO can be employed in the fabrication of several 2,3-BDObased polyesters (Debuissy et al. 2017). Using the succinic acid, 2,3-BDO can be
copolymerized with 1,4-butanediol. 2,3-BDO can be introduced in polyesters to modulate their chemical properties by diminishing their crystallinity and increasing the
glass transition temperature (Debuissy et al. 2016), thus resulting in a codiol of elevated interest for the fabrication, for instance, of thermal adhesives and resins.
The 2,3-BDO production strain in Nozzi et al. (2017) achieved a maximum titer
of 1.6 g/L after 16 days and an overall rate of 100 mg/L/day under enriched CO 2
Fig. 1.6 Chaining up cell factories for CO 2 biotechnological transformation in value-added products. Biotechnological processes can rely on the engineering of multiple microorganisms here
assimilable to intertwined cell factories exchanging substrates and products in complex value
chains
A. A. Azim et al.
Glycerol biotechnological production in CO 2 -fixing microorganisms has been
reported in several studies. For instance, Chow et al. (2013) highlighted the exploitability of the microalga Dunaliella tertiolecta as a natural glycerol producer based
on the observation that extracellular glycerol release acts as a valuable carbon sink
for photosynthetic carbon dioxide capture. The peak concentration amounted to
5.5 g/L upon an 80-day-long cultivation in non-optimized light conditions. In
Savakis et al. (2015), a Synechocystis sp. PCC 6803, engineered by the heterologous
expression of the phosphoglycerol phosphatase 2 encoded by the gpp2 gene from
Saccharomyces cerevisiae, accumulated a glycerol extracellular concentration of
14.3 mM after a 17-day-long cultivation under salt stress condition.
2,3-Butanediol
The chemical compound 2,3-butanediol (2,3-BDO) is gaining share in the global market as an intermediate compound for manufacturing plastics. 2,3-BDO can be transformed with 94% selectivity into 1,3-butadien (Duan et al. 2015), which is a precursor
for a variety of polymer and copolymer materials. The esterification of several dicarboxylic acids with 2,3-BDO can be employed in the fabrication of several 2,3-BDObased polyesters (Debuissy et al. 2017). Using the succinic acid, 2,3-BDO can be
copolymerized with 1,4-butanediol. 2,3-BDO can be introduced in polyesters to modulate their chemical properties by diminishing their crystallinity and increasing the
glass transition temperature (Debuissy et al. 2016), thus resulting in a codiol of elevated interest for the fabrication, for instance, of thermal adhesives and resins.
The 2,3-BDO production strain in Nozzi et al. (2017) achieved a maximum titer
of 1.6 g/L after 16 days and an overall rate of 100 mg/L/day under enriched CO 2
Fig. 1.6 Chaining up cell factories for CO 2 biotechnological transformation in value-added products. Biotechnological processes can rely on the engineering of multiple microorganisms here
assimilable to intertwined cell factories exchanging substrates and products in complex value
chains
A. A. Azim et al.