414
H. Jin
Table 14.2
Aptamers for insulin detection
Name
Modification
Sequence
Produced
In1
Modified with a methylene blue (MB) redox
label at the 3
end
5
-HS-(CH2)6-ACAGGGGTGTGGGGACAGGGGTGTGGGG-C7-MB-3
Naturally
In1-IT
Conjugated to an internal thymine within the
sequence
5
-HS-(CH2)6-ACAGGGGT(C10-MB)GTGGGGACAGGGGTGTGGGG-3
In1-sp
Unmodified DNA probe
5
-ACAGGGGTGTGGGGACAGGGGTGTGGGG-3
IGA3
Unmodified DNA probe
GGTGGTGGGGGGGGTTGGTAGGGTGTCTTC
SELEX