214
T. Zhang et al.
Table 8.2
A summary of aptamer-based assays for the diagnosis of tuberculosis
Assay
Target
Type of aptamer
Limit of detection
Dynamic range
References
Electrochemical assay
CFP10-ESAT6 DNA
10 3
CFU/mL
–
[32]
Electrochemical assay
MPT64
biotinylated aptamer
0.5 fg/mL
–
[36]
Electrochemical assay
H37Rv
AuNPs-DNA
100 CFU/mL
10 2
–10 7
CFU/mL
[33]
Direct dot-blot assay
H37Ra
enzyme linked aptamer
10 4
CFU/mL
–
[31]
GO-based fluorescent assay
Ag85A
ATTO647N-labeled aptamer 1.5 nM in PBS
2.1 nM in serum
5–200 nM
[34]
MOF-rGO-based electrochemical
assay
ESAT-6
DNA
3.3
× 10 −5
ng/mL 1.0
× 10 −4
ng/mL to 2.0
× 10 2
ng/mL
[37]
SWCNT/Au-IDE MSPQC
H37Rv
DNA
100 CFU/mL
1
× 10 3
CFU/mL to 1
× 10 7
CFU/mL
[38]
T. Zhang et al.
Table 8.2
A summary of aptamer-based assays for the diagnosis of tuberculosis
Assay
Target
Type of aptamer
Limit of detection
Dynamic range
References
Electrochemical assay
CFP10-ESAT6 DNA
10 3
CFU/mL
–
[32]
Electrochemical assay
MPT64
biotinylated aptamer
0.5 fg/mL
–
[36]
Electrochemical assay
H37Rv
AuNPs-DNA
100 CFU/mL
10 2
–10 7
CFU/mL
[33]
Direct dot-blot assay
H37Ra
enzyme linked aptamer
10 4
CFU/mL
–
[31]
GO-based fluorescent assay
Ag85A
ATTO647N-labeled aptamer 1.5 nM in PBS
2.1 nM in serum
5–200 nM
[34]
MOF-rGO-based electrochemical
assay
ESAT-6
DNA
3.3
× 10 −5
ng/mL 1.0
× 10 −4
ng/mL to 2.0
× 10 2
ng/mL
[37]
SWCNT/Au-IDE MSPQC
H37Rv
DNA
100 CFU/mL
1
× 10 3
CFU/mL to 1
× 10 7
CFU/mL
[38]