1 Introduction of Aptamer, SELEX, and Different SELEX Variants
11
Fig. 1.7 Capture-SELEX strategy [29]
molecule is ensured, which makes the obtained aptamers have high affinity and specificity for the natural target molecule. However, the library immobilization efficiency
is difficult to control, which will more or less reduce the abundance of library and
the diversity of sequences in the fixing process. This may lead to the loss of some
aptamers with high affinity and specificity in the initial fixed library. In addition,
a dynamic dissociation equilibrium may exist in the library fixing process by base
complementary pairing, which increases the difficulty of screening.
1.3.3.2 GO-SELEX
Graphene Oxide (GO), a member of the graphene family, is a novel two-dimensional
carbon nanomaterial with the thickness of a single atom. Because of its unique
11
Fig. 1.7 Capture-SELEX strategy [29]
molecule is ensured, which makes the obtained aptamers have high affinity and specificity for the natural target molecule. However, the library immobilization efficiency
is difficult to control, which will more or less reduce the abundance of library and
the diversity of sequences in the fixing process. This may lead to the loss of some
aptamers with high affinity and specificity in the initial fixed library. In addition,
a dynamic dissociation equilibrium may exist in the library fixing process by base
complementary pairing, which increases the difficulty of screening.
1.3.3.2 GO-SELEX
Graphene Oxide (GO), a member of the graphene family, is a novel two-dimensional
carbon nanomaterial with the thickness of a single atom. Because of its unique