RP, Niessing D (2017) Molecular architecture
and dynamics of ASH1 mRNA recognition by
its mRNA-transport complex. Nat Struct Mol
Biol 24:152–161
19. Herbert AL, Fu MM, Drerup CM, Gray RS,
Harty BL, Ackerman SD, O’Reilly-Pol T,
Johnson SL, Nechiporuk AV, Barres BA,
Monk KR (2017) Dynein/dynactin is necessary for anterograde transport of Mbp mRNA
in oligodendrocytes and for myelination
in vivo. Proc Natl Acad Sci U S A 114:
E9153–E9162
20. Brendza RP, Serbus LR, Duffy JB, Saxton WM
(2000) A function for kinesin I in the posterior
transport of oskar mRNA and Staufen protein.
Science 289:2120–2122
21. Rook MS, Lu M, Kosik KS (2000) CaMKIIalpha 3
0 untranslated region-directed mRNA
translocation in living neurons: visualization
by GFP linkage. J Neurosci 20:6385–6393
22. Zhang F, Simon AE (2003) A novel procedure
for the localization of viral RNAs in protoplasts
and whole plants. Plant J 35:665–673
23. Sambade A, Brandner K, Hofmann C,
Seemanpillai M, Mutterer J, Heinlein M
(2008) Transport of TMV movement protein
particles associated with the targeting of RNA
to plasmodesmata. Traffic 9:2073–2088
24. Pen ˜a E, Heinlein M, Sambade A (2015) In vivo
RNA labeling using MS2. Methods Mol Biol
1217:329–341
25. Hamada S, Ishiyama K, Choi SB, Wang C,
Singh S, Kawai N, Franceschi VR, Okita TW
(2003) The transport of prolamine RNAs to
prolamine protein bodies in living rice endosperm cells. Plant Cell 15:2253–2264
26. Urbanek
MO,
Galka-Marciniak
P,
Olejniczak M, Krzyzosiak WJ (2014) RNA
imaging in living cells - methods and applications. RNA Biol 11:1083–1095
27. Ko ¨hler RH (1998) GFP for in vivo imaging of
subcellular structures in plant cells. Trends
Plant Sci 3:317–320
28. Luo KR, Huang NC, Yu TS (2018) Selective
targeting of mobile mRNAs to plasmodesmata
for cell-to-cell movement. Plant Physiol
177:604–614
29. Zhou Z, Sim J, Griffith J, Reed R (2002) Purification and electron microscopic visualization
of functional human spliceosomes. Proc Natl
Acad Sci U S A 99:12203–12207
Plant mRNA Live-Cell Imaging Using MS2
155
and dynamics of ASH1 mRNA recognition by
its mRNA-transport complex. Nat Struct Mol
Biol 24:152–161
19. Herbert AL, Fu MM, Drerup CM, Gray RS,
Harty BL, Ackerman SD, O’Reilly-Pol T,
Johnson SL, Nechiporuk AV, Barres BA,
Monk KR (2017) Dynein/dynactin is necessary for anterograde transport of Mbp mRNA
in oligodendrocytes and for myelination
in vivo. Proc Natl Acad Sci U S A 114:
E9153–E9162
20. Brendza RP, Serbus LR, Duffy JB, Saxton WM
(2000) A function for kinesin I in the posterior
transport of oskar mRNA and Staufen protein.
Science 289:2120–2122
21. Rook MS, Lu M, Kosik KS (2000) CaMKIIalpha 3
0 untranslated region-directed mRNA
translocation in living neurons: visualization
by GFP linkage. J Neurosci 20:6385–6393
22. Zhang F, Simon AE (2003) A novel procedure
for the localization of viral RNAs in protoplasts
and whole plants. Plant J 35:665–673
23. Sambade A, Brandner K, Hofmann C,
Seemanpillai M, Mutterer J, Heinlein M
(2008) Transport of TMV movement protein
particles associated with the targeting of RNA
to plasmodesmata. Traffic 9:2073–2088
24. Pen ˜a E, Heinlein M, Sambade A (2015) In vivo
RNA labeling using MS2. Methods Mol Biol
1217:329–341
25. Hamada S, Ishiyama K, Choi SB, Wang C,
Singh S, Kawai N, Franceschi VR, Okita TW
(2003) The transport of prolamine RNAs to
prolamine protein bodies in living rice endosperm cells. Plant Cell 15:2253–2264
26. Urbanek
MO,
Galka-Marciniak
P,
Olejniczak M, Krzyzosiak WJ (2014) RNA
imaging in living cells - methods and applications. RNA Biol 11:1083–1095
27. Ko ¨hler RH (1998) GFP for in vivo imaging of
subcellular structures in plant cells. Trends
Plant Sci 3:317–320
28. Luo KR, Huang NC, Yu TS (2018) Selective
targeting of mobile mRNAs to plasmodesmata
for cell-to-cell movement. Plant Physiol
177:604–614
29. Zhou Z, Sim J, Griffith J, Reed R (2002) Purification and electron microscopic visualization
of functional human spliceosomes. Proc Natl
Acad Sci U S A 99:12203–12207
Plant mRNA Live-Cell Imaging Using MS2
155