INDEX
A
Absorbance ............................. 42, 46, 64, 169, 174, 177,
180, 196, 208, 216, 220–224, 227, 264
B
Bacterial dissemination ................................... iv, 123, 159
Bacterial flagellum ......................................................... 131
Bacterial isolate genome sequence database
(BIGSdb) ................................................ 12–15, 20
Bead assay ...................................................................... 146
Biofilms ................................................................. 207–213
Bioluminescence......... 31, 149, 150, 153, 156, 157, 159
Blood .......... 5, 8, 41, 99, 101, 104, 105, 156, 196, 240,
244–249, 251, 261–268, 277–280
C
Capillary assay....................................................... 124, 126
Cell adhesion ........................................................ 171, 172
Cell-body rotation ............................................... 139–148
Cell viability ................................................................... 222
Cesium chloride purification ....................................68, 71
Chemotaxis...................................................123–129, 142
Cleavage of complement proteins....................... 188, 189
Colony-forming unit (CFU) ................................. 31, 215
Complement regulators ................................................ 188
Complements ............................. 187–190, 193–197, 273
Conjugations ........................77–84, 87, 89–91, 113, 115
Core genome multi-locus sequence typing
(cgMLST) .........................................11–13, 15, 18
Crystal violet.................................................208–210, 213
Cultures .......................... 1–8, 18, 24, 25, 27, 28, 32–35,
37, 45, 56, 57, 61–64, 67–74, 80, 81, 86, 89, 90,
92, 98, 99, 101, 104, 115, 117, 120, 121, 126,
129, 132, 143, 145, 150–152, 161, 163, 165,
166, 168, 177, 189, 190, 194–197, 200–202,
204, 208, 209, 213, 219, 221, 223, 230, 232,
234, 235, 245, 247, 252, 258, 260, 262–264,
267, 271, 278–281, 283
D
dcas9 ..................................................................... 109–121
Diagnosis ................................................. 1, 243, 277–284
Dialysis membrane chambers (DMCs) .............. 230, 231,
233, 235–237, 239, 240
E
Electrical resistance ....................161, 163, 165, 167, 168
Electron cryotomography (ECT) ....................... 132, 133
Electroporation ..................... 77, 78, 112, 113, 115, 117
Enumerations ..................................31–39, 128, 223–225
Enzyme-linked immunosorbent assay (ELISA) .........174,
182, 191, 258, 261, 263, 264, 268, 281, 283
Eukaryotic cells ........................... 39, 109, 161–164, 166,
167, 169, 171
Experimental infections ......................150, 244, 245, 247
Extracellular matrix (ECM)................................. 171, 180
F
Filtration .................... 2, 4, 6, 69, 82, 83, 89, 90, 92, 93,
98, 132, 164, 173, 260
Fitness ......................... 97, 100, 101, 104, 105, 152, 159
Flow cytometry (FCM) ............................................32, 39
Fluorescence ..............33–35, 37, 39, 217, 220–227, 253
Fluorescent dyes ..................................32, 38, 39, 93, 142
G
Gene expression .......................................... 231, 248, 252
Gene silencing ...................................................... 109–121
Genomes........................... 1, 11–20, 31, 42, 67, 93, 109,
149, 150, 250, 251, 258, 268, 274
Genomic libraries ................................................... 98, 102
Guanidinium ...................................................... 42, 44, 48
H
Hamsters...................... 99, 100, 230, 243–254, 257–269
High-throughput sequencing ..................................97, 98
Himar1 ............................................................... 78, 87, 98
Host-adaptation ..................................................... 77, 229
Host-pathogen interaction .................................. 171–184
Hot water ..............................................54, 57, 60, 62, 63
I
Identification .................12, 15, 23–28, 77, 97, 124, 153
Image analysis....................................................... 129, 211
Immunizations ...........................258, 260, 262, 263, 266
Immunofluorescence ........ 201, 202, 204–206, 278, 281
Intracellular trafficking ........................................ 199–206
In vivo live imaging....................................................... 149
Nobuo Koizumi and Mathieu Picardeau (eds.), Leptospira spp.: Methods and Protocols, Methods in Molecular Biology, vol. 2134,
https://doi.org/10.1007/978-1-0716-0459-5, © Springer Science+Business Media, LLC, part of Springer Nature 2020
289
A
Absorbance ............................. 42, 46, 64, 169, 174, 177,
180, 196, 208, 216, 220–224, 227, 264
B
Bacterial dissemination ................................... iv, 123, 159
Bacterial flagellum ......................................................... 131
Bacterial isolate genome sequence database
(BIGSdb) ................................................ 12–15, 20
Bead assay ...................................................................... 146
Biofilms ................................................................. 207–213
Bioluminescence......... 31, 149, 150, 153, 156, 157, 159
Blood .......... 5, 8, 41, 99, 101, 104, 105, 156, 196, 240,
244–249, 251, 261–268, 277–280
C
Capillary assay....................................................... 124, 126
Cell adhesion ........................................................ 171, 172
Cell-body rotation ............................................... 139–148
Cell viability ................................................................... 222
Cesium chloride purification ....................................68, 71
Chemotaxis...................................................123–129, 142
Cleavage of complement proteins....................... 188, 189
Colony-forming unit (CFU) ................................. 31, 215
Complement regulators ................................................ 188
Complements ............................. 187–190, 193–197, 273
Conjugations ........................77–84, 87, 89–91, 113, 115
Core genome multi-locus sequence typing
(cgMLST) .........................................11–13, 15, 18
Crystal violet.................................................208–210, 213
Cultures .......................... 1–8, 18, 24, 25, 27, 28, 32–35,
37, 45, 56, 57, 61–64, 67–74, 80, 81, 86, 89, 90,
92, 98, 99, 101, 104, 115, 117, 120, 121, 126,
129, 132, 143, 145, 150–152, 161, 163, 165,
166, 168, 177, 189, 190, 194–197, 200–202,
204, 208, 209, 213, 219, 221, 223, 230, 232,
234, 235, 245, 247, 252, 258, 260, 262–264,
267, 271, 278–281, 283
D
dcas9 ..................................................................... 109–121
Diagnosis ................................................. 1, 243, 277–284
Dialysis membrane chambers (DMCs) .............. 230, 231,
233, 235–237, 239, 240
E
Electrical resistance ....................161, 163, 165, 167, 168
Electron cryotomography (ECT) ....................... 132, 133
Electroporation ..................... 77, 78, 112, 113, 115, 117
Enumerations ..................................31–39, 128, 223–225
Enzyme-linked immunosorbent assay (ELISA) .........174,
182, 191, 258, 261, 263, 264, 268, 281, 283
Eukaryotic cells ........................... 39, 109, 161–164, 166,
167, 169, 171
Experimental infections ......................150, 244, 245, 247
Extracellular matrix (ECM)................................. 171, 180
F
Filtration .................... 2, 4, 6, 69, 82, 83, 89, 90, 92, 93,
98, 132, 164, 173, 260
Fitness ......................... 97, 100, 101, 104, 105, 152, 159
Flow cytometry (FCM) ............................................32, 39
Fluorescence ..............33–35, 37, 39, 217, 220–227, 253
Fluorescent dyes ..................................32, 38, 39, 93, 142
G
Gene expression .......................................... 231, 248, 252
Gene silencing ...................................................... 109–121
Genomes........................... 1, 11–20, 31, 42, 67, 93, 109,
149, 150, 250, 251, 258, 268, 274
Genomic libraries ................................................... 98, 102
Guanidinium ...................................................... 42, 44, 48
H
Hamsters...................... 99, 100, 230, 243–254, 257–269
High-throughput sequencing ..................................97, 98
Himar1 ............................................................... 78, 87, 98
Host-adaptation ..................................................... 77, 229
Host-pathogen interaction .................................. 171–184
Hot water ..............................................54, 57, 60, 62, 63
I
Identification .................12, 15, 23–28, 77, 97, 124, 153
Image analysis....................................................... 129, 211
Immunizations ...........................258, 260, 262, 263, 266
Immunofluorescence ........ 201, 202, 204–206, 278, 281
Intracellular trafficking ........................................ 199–206
In vivo live imaging....................................................... 149
Nobuo Koizumi and Mathieu Picardeau (eds.), Leptospira spp.: Methods and Protocols, Methods in Molecular Biology, vol. 2134,
https://doi.org/10.1007/978-1-0716-0459-5, © Springer Science+Business Media, LLC, part of Springer Nature 2020
289