Marusic C, Novelli F, Salzano AM, Scaloni A, Benvenuto E, Pioli C, Donini M (2016) Production
of an active anti-CD20-hIL-2 immunocytokine in Nicotiana benthamiana. Plant Biotechnol J
14(1):240–251. https://doi.org/10.1111/pbi.12378
Marusic C, Pioli C, Stelter S, Novelli F, Lonoce C, Morrocchi E, Benvenuto E, Salzano AM,
Scaloni A, Donini M (2018) N-glycan engineering of a plant-produced anti-CD20hIL-2 immunocytokine significantly enhances its effector functions. Biotechnol Bioeng
115(3):565–576. https://doi.org/10.1002/bit.26503
Mason HS, Lam DM, Arntzen CJ (1992) Expression of hepatitis B surface antigen in transgenic
plants. Proc Natl Acad Sci U S A 89(24):11745–11749
Massa S, Franconi R, Brandi R, Muller A, Mett V, Yusibov V, Venuti A (2007) Anti-cancer activity
of plant-produced HPV16 E7 vaccine. Vaccine 25(16):3018–3021. https://doi.org/10.1016/j.
vaccine.2007.01.018
Massa S, Paolini F, Spanò L, Franconi R, Venuti A (2011) Mutants of plant genes for developing
cancer vaccines. Hum Vaccin 7(Suppl):147–155
Massa S, Paolini F, Curzio G, Cordeiro MN, Illiano E, Demurtas OC, Franconi R, Venuti A (2017)
A plant protein signal sequence improved humoral immune response to HPV prophylactic and
therapeutic DNA vaccines. Hum Vaccin Immunother 13(2):271–282. https://doi.org/10.1080/
21645515.2017.1264766
Mayfield SP, Franklin SE, Lerner RA (2003) Expression and assembly of a fully active antibody
in algae. Proc Natl Acad Sci U S A 100:438–442. https://doi.org/10.1073/pnas.0237108100
McCarthy M (2014) US signs contract with ZMapp maker to accelerate development of the Ebola
drug. BMJ 349:g5488. https://doi.org/10.1136/bmj.g5488
McCormick AA, Reddy S, Reinl SJ, Cameron TI, Czerwinkski DK, Vojdani F, Hanley KM,
Garger SJ, White EL, Novak J, Barrett J, Holtz RB, Tusé D, Levy R (2008) Plant-produced
idiotype vaccines for the treatment of non-Hodgkin's lymphoma: safety and immunogenicity in
a phase I clinical study. Proc Natl Acad Sci U S A 105(29):10131–10136. https://doi.org/10.
1073/pnas.0803636105
Mett V, Chichester JA, Stewart ML, Musiychuk K, Bi H, Reifsnyder CJ, Hull AK, Albrecht MT,
Goldman S, Baillie LW, Yusibov V (2011) A non-glycosylated, plant-produced human monoclonal antibody against anthrax protective antigen protects mice and non-human primates from
B. anthracis spore challenge. Hum Vaccin 7:183–190
Meyers B, Zaltsman A, Lacroix B, Kozlovsky SV, Krichevsky A (2010) Nuclear and plastid
genetic engineering of plants: Comparison of opportunities and challenges. Biotechnol Adv
28(6):747–756. https://doi.org/10.1016/j.biotechadv.2010.05.022
Monroy-García A, Gómez-Lim MA, Weiss-Steider B, Hernández-Montes J, Huerta-Yepez S,
Rangel-Santiago JF, Santiago-Osorio E, Mora García Mde L (2014) Immunization with an
HPV-16 L1-based chimeric virus-like particle containing HPV-16 E6 and E7 epitopes elicits
long-lasting prophylactic and therapeutic efficacy in an HPV-16 tumor mice model. Arch Virol
159(2):291–305. https://doi.org/10.1007/s00705-013-1819-z
Musiychuk K, Stephenson N, Bi H, Farrance CE, Orozovic G, Brodelius M, Brodelius P, Horsey A,
Ugulava N, Shamloul AM, Mett V, Rabindran S, Streatfield SJ, Yusibov V (2007) A launch
vector for the production of vaccine antigens in plants. Influenza Other Respir Viruses
1(1):19–25. https://doi.org/10.1111/j.1750-2659.2006.00005.x
Nahampun HN, Bosworth B, Cunnick J, Mogler M, Wang K (2015) Expression of H3N2 nucleoprotein in maize seeds and immunogenicity in mice. Plant Cell Rep 34:969–980. https://doi.
org/10.1007/s00299-015-1758-0
Nandi S, Yalda D, Lu S, Nikolov Z, Misaki R, Fujiyama K, Huang N (2005) Process development
and economic evaluation of recombinant human lactoferrin expressed in rice grain. Transgenic
Res 14(3):237–249
Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S (2013) Targeted mutagenesis in
the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol
31(8):691–693. https://doi.org/10.1038/nbt.2655
Oey M, Lohse M, Kreikemeyer B, Bock R (2009) Exhaustion of the chloroplast protein synthesis
capacity by massive expression of a highly stable protein antibiotic. Plant J 57(3):436–445
Engineering Plants for the Future: Farming with Value-Added Harvest
103
of an active anti-CD20-hIL-2 immunocytokine in Nicotiana benthamiana. Plant Biotechnol J
14(1):240–251. https://doi.org/10.1111/pbi.12378
Marusic C, Pioli C, Stelter S, Novelli F, Lonoce C, Morrocchi E, Benvenuto E, Salzano AM,
Scaloni A, Donini M (2018) N-glycan engineering of a plant-produced anti-CD20hIL-2 immunocytokine significantly enhances its effector functions. Biotechnol Bioeng
115(3):565–576. https://doi.org/10.1002/bit.26503
Mason HS, Lam DM, Arntzen CJ (1992) Expression of hepatitis B surface antigen in transgenic
plants. Proc Natl Acad Sci U S A 89(24):11745–11749
Massa S, Franconi R, Brandi R, Muller A, Mett V, Yusibov V, Venuti A (2007) Anti-cancer activity
of plant-produced HPV16 E7 vaccine. Vaccine 25(16):3018–3021. https://doi.org/10.1016/j.
vaccine.2007.01.018
Massa S, Paolini F, Spanò L, Franconi R, Venuti A (2011) Mutants of plant genes for developing
cancer vaccines. Hum Vaccin 7(Suppl):147–155
Massa S, Paolini F, Curzio G, Cordeiro MN, Illiano E, Demurtas OC, Franconi R, Venuti A (2017)
A plant protein signal sequence improved humoral immune response to HPV prophylactic and
therapeutic DNA vaccines. Hum Vaccin Immunother 13(2):271–282. https://doi.org/10.1080/
21645515.2017.1264766
Mayfield SP, Franklin SE, Lerner RA (2003) Expression and assembly of a fully active antibody
in algae. Proc Natl Acad Sci U S A 100:438–442. https://doi.org/10.1073/pnas.0237108100
McCarthy M (2014) US signs contract with ZMapp maker to accelerate development of the Ebola
drug. BMJ 349:g5488. https://doi.org/10.1136/bmj.g5488
McCormick AA, Reddy S, Reinl SJ, Cameron TI, Czerwinkski DK, Vojdani F, Hanley KM,
Garger SJ, White EL, Novak J, Barrett J, Holtz RB, Tusé D, Levy R (2008) Plant-produced
idiotype vaccines for the treatment of non-Hodgkin's lymphoma: safety and immunogenicity in
a phase I clinical study. Proc Natl Acad Sci U S A 105(29):10131–10136. https://doi.org/10.
1073/pnas.0803636105
Mett V, Chichester JA, Stewart ML, Musiychuk K, Bi H, Reifsnyder CJ, Hull AK, Albrecht MT,
Goldman S, Baillie LW, Yusibov V (2011) A non-glycosylated, plant-produced human monoclonal antibody against anthrax protective antigen protects mice and non-human primates from
B. anthracis spore challenge. Hum Vaccin 7:183–190
Meyers B, Zaltsman A, Lacroix B, Kozlovsky SV, Krichevsky A (2010) Nuclear and plastid
genetic engineering of plants: Comparison of opportunities and challenges. Biotechnol Adv
28(6):747–756. https://doi.org/10.1016/j.biotechadv.2010.05.022
Monroy-García A, Gómez-Lim MA, Weiss-Steider B, Hernández-Montes J, Huerta-Yepez S,
Rangel-Santiago JF, Santiago-Osorio E, Mora García Mde L (2014) Immunization with an
HPV-16 L1-based chimeric virus-like particle containing HPV-16 E6 and E7 epitopes elicits
long-lasting prophylactic and therapeutic efficacy in an HPV-16 tumor mice model. Arch Virol
159(2):291–305. https://doi.org/10.1007/s00705-013-1819-z
Musiychuk K, Stephenson N, Bi H, Farrance CE, Orozovic G, Brodelius M, Brodelius P, Horsey A,
Ugulava N, Shamloul AM, Mett V, Rabindran S, Streatfield SJ, Yusibov V (2007) A launch
vector for the production of vaccine antigens in plants. Influenza Other Respir Viruses
1(1):19–25. https://doi.org/10.1111/j.1750-2659.2006.00005.x
Nahampun HN, Bosworth B, Cunnick J, Mogler M, Wang K (2015) Expression of H3N2 nucleoprotein in maize seeds and immunogenicity in mice. Plant Cell Rep 34:969–980. https://doi.
org/10.1007/s00299-015-1758-0
Nandi S, Yalda D, Lu S, Nikolov Z, Misaki R, Fujiyama K, Huang N (2005) Process development
and economic evaluation of recombinant human lactoferrin expressed in rice grain. Transgenic
Res 14(3):237–249
Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S (2013) Targeted mutagenesis in
the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol
31(8):691–693. https://doi.org/10.1038/nbt.2655
Oey M, Lohse M, Kreikemeyer B, Bock R (2009) Exhaustion of the chloroplast protein synthesis
capacity by massive expression of a highly stable protein antibiotic. Plant J 57(3):436–445
Engineering Plants for the Future: Farming with Value-Added Harvest
103