3.1 Grid Preparation
and Freezing (See
Note 4)
1. Transfer EM grid from box to a clean glass slide using ultra-fine
self-closing tweezers.
2. Glow discharge grid for 20–60 s per side at ~30 mA, following
manufacturer’s instructions.
Fig. 1 General workflow of experiments to prepare samples, collect diffraction
patterns, and process data for structure determination
MicroED of Small Macromolecules
333
and Freezing (See
Note 4)
1. Transfer EM grid from box to a clean glass slide using ultra-fine
self-closing tweezers.
2. Glow discharge grid for 20–60 s per side at ~30 mA, following
manufacturer’s instructions.
Fig. 1 General workflow of experiments to prepare samples, collect diffraction
patterns, and process data for structure determination
MicroED of Small Macromolecules
333