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(7570):486–490. https://doi.org/10.1038/
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Braunschweig AB, Nannenga BL (2020) Crystal structure and orientation of organic semiconductor thin films by microcrystal electron
diffraction and grazing-incidence wide-angle
X-ray scattering. Chem Commun (Camb) 56
(30):4204–4207. https://doi.org/10.1039/
d0cc00119h
18. van Genderen E, Clabbers MT, Das PP,
Stewart A, Nederlof I, Barentsen KC,
Portillo Q, Pannu NS, Nicolopoulos S,
Gruene T, Abrahams JP (2016) Ab initio structure determination of nanocrystals of organic
pharmaceutical compounds by electron diffraction at room temperature using a Timepix
quantum area direct electron detector. Acta
Crystallogr A Found Adv 72(Pt 2):236–242.
https://doi.org/10.1107/
S2053273315022500
19. Gruene T, Wennmacher JTC, Zaubitzer C,
Holstein JJ, Heidler J, Fecteau-Lefebvre A,
De Carlo S, Muller E, Goldie KN, Regeni I,
Li T, Santiso-Quinones G, Steinfeld G,
Handschin S, van Genderen E, van Bokhoven
JA, Clever GH, Pantelic R (2018) Rapid structure determination of microcrystalline molecular compounds using electron diffraction.
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Chem
Int
Ed
Eng
57
(50):16313–16317.
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1002/anie.201811318
20. Ting CP, Funk MA, Halaby SL, Zhang Z,
Gonen T, van der Donk WA (2019) Use of a
scaffold peptide in the biosynthesis of amino
acid–derived natural products. Science 365
(6450):280.
https://doi.org/10.1126/sci
ence.aau6232
21. Jones CG, Martynowycz MW, Hattne J, Fulton TJ, Stoltz BM, Rodriguez JA, Nelson HM,
Gonen T (2018) The cryoEM method
microED as a powerful tool for small molecule
structure determination. ACS Central Sci 4
(11):1587–1592. https://doi.org/10.1021/
acscentsci.8b00760
22. Shi D, Nannenga BL, de la Cruz MJ, Liu J,
Sawtelle S, Calero G, Reyes FE, Hattne J,
Gonen T (2016) The collection of MicroED
data for macromolecular crystallography. Nat
Protoc 11(5):895–904. https://doi.org/10.
1038/nprot.2016.046
23. Carragher B, Cheng Y, Frost A, Glaeser RM,
Lander GC, Nogales E, Wang HW (2019)
Current outcomes when optimizing ‘standard’
sample preparation for single-particle cryoEM. J Microsc 276(1):39–45. https://doi.
org/10.1111/jmi.12834
24. Martynowycz MW, Zhao W, Hattne J, Jensen
GJ, Gonen T (2019) Collection of continuous
rotation MicroED data from ion beam-milled
crystals of any size. Structure 27(3):545–548.
e542.
https://doi.org/10.1016/j.str.2018.
12.003
25. Duyvesteyn HME, Kotecha A, Ginn HM,
Hecksel CW, Beale EV, de Haas F, Evans G,
Zhang P, Chiu W, Stuart DI (2018) Machining
protein microcrystals for structure determination by electron diffraction. Proc Natl Acad Sci
U S A 115(38):9569–9573. https://doi.org/
10.1073/pnas.1809978115
26. de la Cruz MJ, Martynowycz MW, Hattne J,
Gonen T (2019) MicroED data collection with
SerialEM.
Ultramicroscopy
201:77–80.
https://doi.org/10.1016/j.ultramic.2019.03.
009
27. Wang B, Zou X, Smeets S (2019) Automated
serial rotation electron diffraction combined
with cluster analysis: an efficient multi-crystal
workflow for structure determination. IUCrJ 6
(Pt 5):854–867. https://doi.org/10.1107/
S2052252519007681
28. Cichocka MO, Angstrom J, Wang B, Zou X,
Smeets S (2018) High-throughput continuous
rotation electron diffraction data acquisition
via software automation. J Appl Crystallogr
51(Pt 6):1652–1661. https://doi.org/10.
1107/S1600576718015145
29. Hattne J, Reyes FE, Nannenga BL, Shi D, de la
Cruz MJ, Leslie AG, Gonen T (2015)
MicroED data collection and processing. Acta
Crystallogr A Found Adv 71(Pt 4):353–360.
https://doi.org/10.1107/
S2053273315010669
30. Clabbers MTB, Gruene T, Parkhurst JM, Abrahams JP, Waterman DG (2018) Electron diffraction data processing with DIALS. Acta
Crystallogr D Struct Biol 74(Pt 6):506–518.
https://doi.org/10.1107/
S2059798318007726
31. Stevenson HP, Lin G, Barnes CO,
Sutkeviciute I, Krzysiak T, Weiss SC,
Reynolds S, Wu Y, Nagarajan V, Makhov AM,
Lawrence R, Lamm E, Clark L, Gardella TJ,
Hogue BG, Ogata CM, Ahn J, Gronenborn
AM, Conway JF, Vilardaga JP, Cohen AE,
Calero G (2016) Transmission electron
microscopy for the evaluation and optimization of crystal growth. Acta Crystallogr D
Struct Biol 72(Pt 5):603–615. https://doi.
org/10.1107/S2059798316001546
32. Barnes CO, Kovaleva EG, Fu X, Stevenson HP,
Brewster AS, DePonte DP, Baxter EL, Cohen
AE, Calero G (2016) Assessment of
296
Guanhong Bu and Brent L. Nannenga
(7570):486–490. https://doi.org/10.1038/
nature15368
17. Levine AM, Bu G, Biswas S, Tsai EHR,
Braunschweig AB, Nannenga BL (2020) Crystal structure and orientation of organic semiconductor thin films by microcrystal electron
diffraction and grazing-incidence wide-angle
X-ray scattering. Chem Commun (Camb) 56
(30):4204–4207. https://doi.org/10.1039/
d0cc00119h
18. van Genderen E, Clabbers MT, Das PP,
Stewart A, Nederlof I, Barentsen KC,
Portillo Q, Pannu NS, Nicolopoulos S,
Gruene T, Abrahams JP (2016) Ab initio structure determination of nanocrystals of organic
pharmaceutical compounds by electron diffraction at room temperature using a Timepix
quantum area direct electron detector. Acta
Crystallogr A Found Adv 72(Pt 2):236–242.
https://doi.org/10.1107/
S2053273315022500
19. Gruene T, Wennmacher JTC, Zaubitzer C,
Holstein JJ, Heidler J, Fecteau-Lefebvre A,
De Carlo S, Muller E, Goldie KN, Regeni I,
Li T, Santiso-Quinones G, Steinfeld G,
Handschin S, van Genderen E, van Bokhoven
JA, Clever GH, Pantelic R (2018) Rapid structure determination of microcrystalline molecular compounds using electron diffraction.
Angew
Chem
Int
Ed
Eng
57
(50):16313–16317.
https://doi.org/10.
1002/anie.201811318
20. Ting CP, Funk MA, Halaby SL, Zhang Z,
Gonen T, van der Donk WA (2019) Use of a
scaffold peptide in the biosynthesis of amino
acid–derived natural products. Science 365
(6450):280.
https://doi.org/10.1126/sci
ence.aau6232
21. Jones CG, Martynowycz MW, Hattne J, Fulton TJ, Stoltz BM, Rodriguez JA, Nelson HM,
Gonen T (2018) The cryoEM method
microED as a powerful tool for small molecule
structure determination. ACS Central Sci 4
(11):1587–1592. https://doi.org/10.1021/
acscentsci.8b00760
22. Shi D, Nannenga BL, de la Cruz MJ, Liu J,
Sawtelle S, Calero G, Reyes FE, Hattne J,
Gonen T (2016) The collection of MicroED
data for macromolecular crystallography. Nat
Protoc 11(5):895–904. https://doi.org/10.
1038/nprot.2016.046
23. Carragher B, Cheng Y, Frost A, Glaeser RM,
Lander GC, Nogales E, Wang HW (2019)
Current outcomes when optimizing ‘standard’
sample preparation for single-particle cryoEM. J Microsc 276(1):39–45. https://doi.
org/10.1111/jmi.12834
24. Martynowycz MW, Zhao W, Hattne J, Jensen
GJ, Gonen T (2019) Collection of continuous
rotation MicroED data from ion beam-milled
crystals of any size. Structure 27(3):545–548.
e542.
https://doi.org/10.1016/j.str.2018.
12.003
25. Duyvesteyn HME, Kotecha A, Ginn HM,
Hecksel CW, Beale EV, de Haas F, Evans G,
Zhang P, Chiu W, Stuart DI (2018) Machining
protein microcrystals for structure determination by electron diffraction. Proc Natl Acad Sci
U S A 115(38):9569–9573. https://doi.org/
10.1073/pnas.1809978115
26. de la Cruz MJ, Martynowycz MW, Hattne J,
Gonen T (2019) MicroED data collection with
SerialEM.
Ultramicroscopy
201:77–80.
https://doi.org/10.1016/j.ultramic.2019.03.
009
27. Wang B, Zou X, Smeets S (2019) Automated
serial rotation electron diffraction combined
with cluster analysis: an efficient multi-crystal
workflow for structure determination. IUCrJ 6
(Pt 5):854–867. https://doi.org/10.1107/
S2052252519007681
28. Cichocka MO, Angstrom J, Wang B, Zou X,
Smeets S (2018) High-throughput continuous
rotation electron diffraction data acquisition
via software automation. J Appl Crystallogr
51(Pt 6):1652–1661. https://doi.org/10.
1107/S1600576718015145
29. Hattne J, Reyes FE, Nannenga BL, Shi D, de la
Cruz MJ, Leslie AG, Gonen T (2015)
MicroED data collection and processing. Acta
Crystallogr A Found Adv 71(Pt 4):353–360.
https://doi.org/10.1107/
S2053273315010669
30. Clabbers MTB, Gruene T, Parkhurst JM, Abrahams JP, Waterman DG (2018) Electron diffraction data processing with DIALS. Acta
Crystallogr D Struct Biol 74(Pt 6):506–518.
https://doi.org/10.1107/
S2059798318007726
31. Stevenson HP, Lin G, Barnes CO,
Sutkeviciute I, Krzysiak T, Weiss SC,
Reynolds S, Wu Y, Nagarajan V, Makhov AM,
Lawrence R, Lamm E, Clark L, Gardella TJ,
Hogue BG, Ogata CM, Ahn J, Gronenborn
AM, Conway JF, Vilardaga JP, Cohen AE,
Calero G (2016) Transmission electron
microscopy for the evaluation and optimization of crystal growth. Acta Crystallogr D
Struct Biol 72(Pt 5):603–615. https://doi.
org/10.1107/S2059798316001546
32. Barnes CO, Kovaleva EG, Fu X, Stevenson HP,
Brewster AS, DePonte DP, Baxter EL, Cohen
AE, Calero G (2016) Assessment of
296
Guanhong Bu and Brent L. Nannenga