Fig. 1 Examples of different particles stained with UF and a comparison with CryoEM. (a) A designed protein
tetrahedron. (b) Taxol-stabilized microtubule. (c) Microtubules with bound Dam1 rings. (d) Membrane protein
TRPV1. (e) A designed icosahedrally symmetric protein cage highlighting the possible flattening effect of stain
(f) Micrograph of the same particles in (e) frozen in ice for CryoEM without flattening
Fig. 2 UF Stained protein crystals. (a) A designed 2D protein crystal with a power spectrum (inset) showing
reflections. (b) Three-dimensional crystals exhibiting needle morphology
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Shane Gonen