40
fumigatus, whereas A. nidulans is less pathogenic and is also involved in iron
metabolism of filamentous fungi (Haas 2012). From the available information, a
network of A. nidulans and A. fumigatus was constructed using Cytoscape 3.7.1
with genes and protein targets involved in biosynthetic pathway which are shown in
Figs. 3.1 and 3.2, respectively.
Common genes between A. fumigatus and A. nidulans network are shown in
Fig. 3.3. Genes such as mirB, mirC, sidD, sidL, and sidF are commonly occurring
in both the species and have common protein targets. Low-affinity iron acquisition
and siderophore-aided high-affinity iron uptake are acquired by both A. fumigatus
and A. nidulans (Schrettl et al. 2004).
Triacetylfusarinine C is composed of three N2-acetyl-N5-anhydromevalonylN5- hydroxyornithine residues which are further cyclically connected by ester bonds
Table 3.2 (continued)
Aspergillus
spp.
Associated genes
Siderophore activity
A. niger
An03g01550
Siderophore biosynthetic process
An05g01780,
An04g05570
Domain with siderophore transmembrane transporter
activity
An16g01150
Ferric-chelate reductase activity involved in
siderophore transport
An01g00720
Helps in transport of siderophores
An03g03530,
An16g04320
Involved in hydrolase activity and siderophore
catabolic process
sidF
Siderophore biosynthetic pathway
An02g14190
Involved in siderophore transmembrane transport
An01g02370
Involved in iron transport and siderophore
biosynthetic pathway
An12g05510
Siderophore iron transporter
A. oryzae
AO090701000976
Plays an important role in siderophore biosynthetic
process
dffA
L-Ornithine N5-oxygenase involved in siderophore
biosynthesis
AO090701000932
Siderophore transportation and plasma membrane
localization
AO090124000035
Helps in siderophore transport and plasma membrane
localization
AO090005000471
Helps in ferrichrome transport and transmembrane
transport
AO090103000170,
AO090701000114
Essential for hydrolase activity, acting on ester bonds
and in siderophore catabolic process
sidF
Siderophore biosynthetic process and peroxisome
localization
AO090003000906
Siderophore biosynthetic process and cytoplasm
localization
AO090001000692
Siderophore transmembrane transport
sidC
Putative NRPS; ortholog of the siderophore
biosynthetic enzyme of A. fumigatus
S. Jayapalan et al.
fumigatus, whereas A. nidulans is less pathogenic and is also involved in iron
metabolism of filamentous fungi (Haas 2012). From the available information, a
network of A. nidulans and A. fumigatus was constructed using Cytoscape 3.7.1
with genes and protein targets involved in biosynthetic pathway which are shown in
Figs. 3.1 and 3.2, respectively.
Common genes between A. fumigatus and A. nidulans network are shown in
Fig. 3.3. Genes such as mirB, mirC, sidD, sidL, and sidF are commonly occurring
in both the species and have common protein targets. Low-affinity iron acquisition
and siderophore-aided high-affinity iron uptake are acquired by both A. fumigatus
and A. nidulans (Schrettl et al. 2004).
Triacetylfusarinine C is composed of three N2-acetyl-N5-anhydromevalonylN5- hydroxyornithine residues which are further cyclically connected by ester bonds
Table 3.2 (continued)
Aspergillus
spp.
Associated genes
Siderophore activity
A. niger
An03g01550
Siderophore biosynthetic process
An05g01780,
An04g05570
Domain with siderophore transmembrane transporter
activity
An16g01150
Ferric-chelate reductase activity involved in
siderophore transport
An01g00720
Helps in transport of siderophores
An03g03530,
An16g04320
Involved in hydrolase activity and siderophore
catabolic process
sidF
Siderophore biosynthetic pathway
An02g14190
Involved in siderophore transmembrane transport
An01g02370
Involved in iron transport and siderophore
biosynthetic pathway
An12g05510
Siderophore iron transporter
A. oryzae
AO090701000976
Plays an important role in siderophore biosynthetic
process
dffA
L-Ornithine N5-oxygenase involved in siderophore
biosynthesis
AO090701000932
Siderophore transportation and plasma membrane
localization
AO090124000035
Helps in siderophore transport and plasma membrane
localization
AO090005000471
Helps in ferrichrome transport and transmembrane
transport
AO090103000170,
AO090701000114
Essential for hydrolase activity, acting on ester bonds
and in siderophore catabolic process
sidF
Siderophore biosynthetic process and peroxisome
localization
AO090003000906
Siderophore biosynthetic process and cytoplasm
localization
AO090001000692
Siderophore transmembrane transport
sidC
Putative NRPS; ortholog of the siderophore
biosynthetic enzyme of A. fumigatus
S. Jayapalan et al.