20. Stupfler B, Birck C, Seraphin B, Mauxion F
(2016) BTG2 bridges PABPC1 RNA-binding
domains and CAF1 deadenylase to control cell
proliferation. Nat Commun 7:10811
21. Balbo A, Minor KH, Velikovsky CA, Mariuzza
RA, Peterson CB, Schuck P (2005) Studying
multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation. Proc Natl Acad Sci U S A 102:81–86
22. Zhao H, Piszczek G, Schuck P (2015) SEDPHAT--a platform for global ITC analysis and
global multi-method analysis of molecular
interactions. Methods 76:137–148
23. Zhao H, Schuck P (2015) Combining biophysical methods for the analysis of protein complex
stoichiometry and affinity in SEDPHAT. Acta
Crystallogr D Biol Crystallogr 71:3–14
24. Brautigam CA (2015) Calculations and
publication-quality illustrations for analytical
ultracentrifugation data. Methods Enzymol
562:109–133
25. Schuck P (2010) Sedimentation patterns of
rapidly reversible protein interactions. Biophys
J 98:2005–2013
26. Demeler B (2010) Methods for the design and
analysis of sedimentation velocity and sedimentation equilibrium experiments with proteins.
Curr Protoc Protein Sci. Chapter 7, Unit 7.13
Heterogeneity and Affinity Interaction Analysis by Sedimentation Velocity
171
(2016) BTG2 bridges PABPC1 RNA-binding
domains and CAF1 deadenylase to control cell
proliferation. Nat Commun 7:10811
21. Balbo A, Minor KH, Velikovsky CA, Mariuzza
RA, Peterson CB, Schuck P (2005) Studying
multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation. Proc Natl Acad Sci U S A 102:81–86
22. Zhao H, Piszczek G, Schuck P (2015) SEDPHAT--a platform for global ITC analysis and
global multi-method analysis of molecular
interactions. Methods 76:137–148
23. Zhao H, Schuck P (2015) Combining biophysical methods for the analysis of protein complex
stoichiometry and affinity in SEDPHAT. Acta
Crystallogr D Biol Crystallogr 71:3–14
24. Brautigam CA (2015) Calculations and
publication-quality illustrations for analytical
ultracentrifugation data. Methods Enzymol
562:109–133
25. Schuck P (2010) Sedimentation patterns of
rapidly reversible protein interactions. Biophys
J 98:2005–2013
26. Demeler B (2010) Methods for the design and
analysis of sedimentation velocity and sedimentation equilibrium experiments with proteins.
Curr Protoc Protein Sci. Chapter 7, Unit 7.13
Heterogeneity and Affinity Interaction Analysis by Sedimentation Velocity
171
