References
1. Arthur KK, Kendrick BS, Gabrielson JP (2015)
Guidance to achieve accurate aggregate quantitation in biopharmaceuticals by SV-AUC.
Methods Enzymol 562:477–500
2. Correia JJ, Stafford WF (2015) Sedimentation
velocity: a classical perspective. Methods Enzymol 562:49–80
3. Zhao H, Mayer ML, Schuck P (2014) Analysis
of protein interactions with picomolar binding
affinity by fluorescence-detected sedimentation
velocity. Anal Chem 86:3181–3187
4. Kingsbury JS, Laue TM (2011) Fluorescencedetected sedimentation in dilute and highly
concentrated solutions. Methods Enzymol
492:283–304
5. Schuck P (2013) Analytical ultracentrifugation
as a tool for studying protein interactions. Biophys Rev 5:159–171
6. Le Roy A, Wang K, Schaack B, Schuck P,
Breyton C, Ebel C (2015) AUC and smallangle scattering for membrane proteins. Methods Enzymol 562:257–286
7. Unzai S (2018) Analytical ultracentrifugation
in structural biology. Biophys Rev 10:229–233
8. Salvay AG, Communie G, Ebel C (2012) Sedimentation velocity analytical ultracentrifugation for intrinsically disordered proteins.
Methods Mol Biol 896:91–105
9. Tabarani G, Reina JJ, Ebel C, Vive `s C, LortatJacob H, Rojo J, Fieschi F (2006) Mannose
hyperbranched dendritic polymers interact
with clustered organization of DC-SIGN and
inhibit
gp120
binding.
FEBS
Lett
580:2402–2408
10. Dach I, Olesen C, Signor L, Nissen P, le
Maire M, Møller JV, Ebel C (2012) Active
detergent-solubilized H+,K+-ATPase is a
monomer. J Biol Chem 287:41963–41978
11. Rocco M, Byron O (2015) Computing translational diffusion and sedimentation coefficients: an evaluation of experimental data and
programs. Eur Biophys J 44:417–431
12. Chaturvedi SK, Ma J, Brown PH, Zhao H,
Schuck P (2018) Measuring macromolecular
size distributions and interactions at high concentrations by sedimentation velocity. Nat
Commun 9:4415
13. Lebowitz J, Lewis MS, Schuck P (2002) Modern analytical ultracentrifugation in protein science: a tutorial review. Protein Sci
11:2067–2079
14. Schuck P (2000) Size-distribution analysis of
macromolecules by sedimentation velocity
ultracentrifugation and lamm equation modeling. Biophys J 78:1606–1619
15. Dam J, Schuck P (2004) Calculating sedimentation coefficient distributions by direct modeling of sedimentation velocity concentration
profiles. Methods Enzymol 384:185–212
16. Zhao H, Ghirlando R, Alfonso C, Arisaka F,
Attali I, Bain DL, Bakhtina MM, Becker DF,
Bedwell GJ, Bekdemir A, Besong TMD,
Birck C, Brautigam CA, Brennerman W,
Byron O, Bzowska A, Chaires JB, Chaton CT,
Co ¨lfen H, Connaghan KD, Crowley KA,
Curth U, Daviter T, Dean WL, Dı ´ez AI,
Ebel C, Eckert DM, Eisele LE, Eisenstein E,
England P, Escalante C, Fagan JA, Fairman R,
Finn RM, Fischle W, de la Torre JG, Gor J,
Gustafsson H, Hall D, Harding SE, Cifre
JGH, Herr AB, Howell EE, Isaac RS, Jao
S-C, Jose D, Kim S-J, Kokona B, Kornblatt
JA, Kosek D, Krayukhina E, Krzizike D, Kusznir EA, Kwon H, Larson A, Laue TM, Le
Roy A, Leech AP, Lilie H, Luger K, LuqueOrtega JR, Ma J, May CA, Maynard EL,
Modrak-Wojcik A, Mok Y-F, Mu ¨cke N,
Nagel-Steger L, Narlikar GJ, Noda M,
Nourse A, Obsil T, Park CK, Park J-K, Pawelek
PD, Perdue EE, Perkins SJ, Perugini MA,
Peterson CL, Peverelli MG, Piszczek G,
Prag G, Prevelige PE, Raynal BDE,
Rezabkova L, Richter K, Ringel AE,
Rosenberg R, Rowe AJ, Rufer AC, Scott DJ,
Seravalli JG, Solovyova AS, Song R,
Staunton D, Stoddard C, Stott K, Strauss
HM, Streicher WW, Sumida JP, Swygert SG,
Szczepanowski RH, Tessmer I, Toth RT,
Tripathy A, Uchiyama S, Uebel SFW,
Unzai S, Gruber AV, von Hippel PH,
Wandrey C, Wang S-H, Weitzel SE, WielgusKutrowska B, Wolberger C, Wolff M, Wright E,
Wu Y-S, Wubben JM, Schuck P (2015) A multilaboratory comparison of calibration accuracy
and the performance of external references in
analytical ultracentrifugation. PLoS One 10:
e0126420
17. Zhao H, Balbo A, Brown PH, Schuck P (2011)
The boundary structure in the analysis of reversibly interacting systems by sedimentation
velocity. Methods 54:16–30
18. Brautigam CA (2011) Using Lamm-equation
modeling of sedimentation velocity data to
determine the kinetic and thermodynamic
properties of macromolecular interactions.
Methods 54:4–15
19. Zhao H, Brautigam CA, Ghirlando R, Schuck
P (2013) Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation. Curr
Protoc Protein Sci. Chapter 20, Unit20.12
170
Christine Ebel and Catherine Birck
1. Arthur KK, Kendrick BS, Gabrielson JP (2015)
Guidance to achieve accurate aggregate quantitation in biopharmaceuticals by SV-AUC.
Methods Enzymol 562:477–500
2. Correia JJ, Stafford WF (2015) Sedimentation
velocity: a classical perspective. Methods Enzymol 562:49–80
3. Zhao H, Mayer ML, Schuck P (2014) Analysis
of protein interactions with picomolar binding
affinity by fluorescence-detected sedimentation
velocity. Anal Chem 86:3181–3187
4. Kingsbury JS, Laue TM (2011) Fluorescencedetected sedimentation in dilute and highly
concentrated solutions. Methods Enzymol
492:283–304
5. Schuck P (2013) Analytical ultracentrifugation
as a tool for studying protein interactions. Biophys Rev 5:159–171
6. Le Roy A, Wang K, Schaack B, Schuck P,
Breyton C, Ebel C (2015) AUC and smallangle scattering for membrane proteins. Methods Enzymol 562:257–286
7. Unzai S (2018) Analytical ultracentrifugation
in structural biology. Biophys Rev 10:229–233
8. Salvay AG, Communie G, Ebel C (2012) Sedimentation velocity analytical ultracentrifugation for intrinsically disordered proteins.
Methods Mol Biol 896:91–105
9. Tabarani G, Reina JJ, Ebel C, Vive `s C, LortatJacob H, Rojo J, Fieschi F (2006) Mannose
hyperbranched dendritic polymers interact
with clustered organization of DC-SIGN and
inhibit
gp120
binding.
FEBS
Lett
580:2402–2408
10. Dach I, Olesen C, Signor L, Nissen P, le
Maire M, Møller JV, Ebel C (2012) Active
detergent-solubilized H+,K+-ATPase is a
monomer. J Biol Chem 287:41963–41978
11. Rocco M, Byron O (2015) Computing translational diffusion and sedimentation coefficients: an evaluation of experimental data and
programs. Eur Biophys J 44:417–431
12. Chaturvedi SK, Ma J, Brown PH, Zhao H,
Schuck P (2018) Measuring macromolecular
size distributions and interactions at high concentrations by sedimentation velocity. Nat
Commun 9:4415
13. Lebowitz J, Lewis MS, Schuck P (2002) Modern analytical ultracentrifugation in protein science: a tutorial review. Protein Sci
11:2067–2079
14. Schuck P (2000) Size-distribution analysis of
macromolecules by sedimentation velocity
ultracentrifugation and lamm equation modeling. Biophys J 78:1606–1619
15. Dam J, Schuck P (2004) Calculating sedimentation coefficient distributions by direct modeling of sedimentation velocity concentration
profiles. Methods Enzymol 384:185–212
16. Zhao H, Ghirlando R, Alfonso C, Arisaka F,
Attali I, Bain DL, Bakhtina MM, Becker DF,
Bedwell GJ, Bekdemir A, Besong TMD,
Birck C, Brautigam CA, Brennerman W,
Byron O, Bzowska A, Chaires JB, Chaton CT,
Co ¨lfen H, Connaghan KD, Crowley KA,
Curth U, Daviter T, Dean WL, Dı ´ez AI,
Ebel C, Eckert DM, Eisele LE, Eisenstein E,
England P, Escalante C, Fagan JA, Fairman R,
Finn RM, Fischle W, de la Torre JG, Gor J,
Gustafsson H, Hall D, Harding SE, Cifre
JGH, Herr AB, Howell EE, Isaac RS, Jao
S-C, Jose D, Kim S-J, Kokona B, Kornblatt
JA, Kosek D, Krayukhina E, Krzizike D, Kusznir EA, Kwon H, Larson A, Laue TM, Le
Roy A, Leech AP, Lilie H, Luger K, LuqueOrtega JR, Ma J, May CA, Maynard EL,
Modrak-Wojcik A, Mok Y-F, Mu ¨cke N,
Nagel-Steger L, Narlikar GJ, Noda M,
Nourse A, Obsil T, Park CK, Park J-K, Pawelek
PD, Perdue EE, Perkins SJ, Perugini MA,
Peterson CL, Peverelli MG, Piszczek G,
Prag G, Prevelige PE, Raynal BDE,
Rezabkova L, Richter K, Ringel AE,
Rosenberg R, Rowe AJ, Rufer AC, Scott DJ,
Seravalli JG, Solovyova AS, Song R,
Staunton D, Stoddard C, Stott K, Strauss
HM, Streicher WW, Sumida JP, Swygert SG,
Szczepanowski RH, Tessmer I, Toth RT,
Tripathy A, Uchiyama S, Uebel SFW,
Unzai S, Gruber AV, von Hippel PH,
Wandrey C, Wang S-H, Weitzel SE, WielgusKutrowska B, Wolberger C, Wolff M, Wright E,
Wu Y-S, Wubben JM, Schuck P (2015) A multilaboratory comparison of calibration accuracy
and the performance of external references in
analytical ultracentrifugation. PLoS One 10:
e0126420
17. Zhao H, Balbo A, Brown PH, Schuck P (2011)
The boundary structure in the analysis of reversibly interacting systems by sedimentation
velocity. Methods 54:16–30
18. Brautigam CA (2011) Using Lamm-equation
modeling of sedimentation velocity data to
determine the kinetic and thermodynamic
properties of macromolecular interactions.
Methods 54:4–15
19. Zhao H, Brautigam CA, Ghirlando R, Schuck
P (2013) Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation. Curr
Protoc Protein Sci. Chapter 20, Unit20.12
170
Christine Ebel and Catherine Birck