of ten heavy metals using the duckweed growth
inhibition test (ISO 20079) with Lemna minor L.
clone St. J Plant Physiol 164(12):1656–1664
Olah V, Hepp A, Meszaros I (2016) Assessment of giant
duckweed (Spirodela polyrhiza L. Schleiden) turions
as model objects in ecotoxicological applications. Bull
Environ Contam Toxicol 96(5):596–601
Ozengin N, Elmaci A (2007) Performance of duckweed
(Lemna minor L.) on different types of wastewater
treatment. J Environ Biol 28(2):307–314
Rahman MA, Hasegawa H (2011) Aquatic arsenic:
phytoremediation using floating macrophytes. Chemosphere 83(5):633–646
Rai UN, Sinha S, Tripathi RD, Chandra P (1995)
Waste-water treatability potential of some aquatic
macrophytes—removal of heavy-metals. Ecol Eng 5
(1):5–12
Sato S, Tabata S, Hirakawa H, Asamizu E, Shirasawa K,
Isobe S, Kaneko T, Nakamura Y, Shibata D, Aoki K,
Egholm M, Knight J, Bogden R, Li CB, Shuang Y,
Xu X, Pan SK, Cheng SF, Liu X, Ren YY, Wang J,
Albiero A, Dal Pero F, Todesco S, Van Eck J,
Buels RM, Bombarely A, Gosselin JR, Huang MY,
Leto JA, Menda N, Strickler S, Mao LY, Gao S,
Tecle IY, York T, Zheng Y, Vrebalov JT, Lee J,
Zhong SL, Mueller LA, Stiekema WJ, Ribeca P,
Alioto T, Yang WC, Huang SW, Du YC, Zhang ZH,
Gao JC, Guo YM, Wang XX, Li Y, He J, Li CY,
Cheng ZK, Zuo JR, Ren JF, Zhao JH, Yan LH,
Jiang HL, Wang B, Li HS, Li ZJ, Fu FY, Chen BT,
Han B, Feng Q, Fan DL, Wang Y, Ling HQ,
Xue YBA, Ware D, McCombie WR, Lippman ZB,
Chia JM, Jiang K, Pasternak S, Gelley L, Kramer M,
Anderson LK, Chang SB, Royer SM, Shearer LA,
Stack SM, Rose JKC, Xu YM, Eannetta N, Matas AJ,
McQuinn R, Tanksley SD, Camara F, Guigo R,
Rombauts S, Fawcett J, Van de Peer Y, Zamir D,
Liang CB, Spannagl M, Gundlach H, Bruggmann R,
Mayer K, Jia ZQ, Zhang JH, Ye ZBA, Bishop GJ,
Butcher S, Lopez-Cobollo R, Buchan D, Filippis I,
Abbott J, Dixit R, Singh M, Singh A, Pal JK, Pandit A,
Singh PK, Mahato AK, Dogra V, Gaikwad K,
Sharma TR, Mohapatra T, Singh NK, Causse M,
Rothan C, Schiex T, Noirot C, Bellec A, Klopp C,
Delalande C, Berges H, Mariette J, Frasse P,
Vautrin S, Zouine M, Latche A, Rousseau C,
Regad F, Pech JC, Philippot M, Bouzayen M, Pericard P, Osorio S, del Carmen AF, Monforte A,
Granell A, Fernandez-Munoz R, Conte M, Lichtenstein G, Carrari F, De Bellis G, Fuligni F, Peano C,
Grandillo S, Termolino P, Pietrella M, Fantini E,
Falcone G, Fiore A, Giuliano G, Lopez L, Facella P,
Perrotta G, Daddiego L, Bryan G, Orozco M, Pastor X,
Torrents D, van Schriek KNVMGM, Feron RMC, van
Oeveren J, de Heer P, daPonte L, Jacobs-Oomen S,
Cariaso M, Prins M, van Eijk MJT, Janssen A, van
Haaren MJJ, Jo SH, Kim J, Kwon SY, Kim S,
Koo DH, Lee S, Hur CG, Clouser C, Rico A,
Hallab A, Gebhardt C, Klee K, Jocker A, Warfsmann J, Gobel U, Kawamura S, Yano K, Sherman JD,
Fukuoka H, Negoro S, Bhutty S, Chowdhury P,
Chattopadhyay D, Datema E, Smit S, Schijlen EWM,
van de Belt J, van Haarst JC, Peters SA, van
Staveren MJ, Henkens MHC, Mooyman PJW, Hesselink T, van Ham RCHJ, Jiang GY, Droege M,
Choi D, Kang BC, Kim BD, Park M, Kim S, Yeom SI,
Lee YH, Choi YD, Li GC, Gao JW, Liu YS,
Huang SX, Fernandez-Pedrosa V, Collado C,
Zuniga S, Wang GP, Cade R, Dietrich RA,
Rogers J, Knapp S, Fei ZJ, White RA,
Thannhauser TW, Giovannoni JJ, Botella MA, Gilbert L, Gonzalez R, Goicoechea JL, Yu Y, Kudrna D,
Collura K, Wissotski M, Wing R, Schoof H, Meyers BC, Gurazada AB, Green PJ, Mathur S, Vyas S,
Solanke AU, Kumar R, Gupta V, Sharma AK,
Khurana P, Khurana JP, Tyagi AK, Dalmay T,
Mohorianu I, Walts B, Chamala S, Barbazuk WB,
Li JP, Guo H, Lee TH, Wang YP, Zhang D,
Paterson AH, Wang XY, Tang HB, Barone A,
Chiusano ML, Ercolano MR, D’Agostino N, Di
Filippo M, Traini A, Sanseverino W, Frusciante L,
Seymour GB, Elharam M, Fu Y, Hua A, Kenton S,
Lewis J, Lin SP, Najar F, Lai HS, Qin BF, Qu CM,
Shi RH, White D, White J, Xing YB, Yang KQ, Yi J,
Yao ZY, Zhou LP, Roe BA, Vezzi A, D’Angelo M,
Zimbello R, Schiavon R, Caniato E, Rigobello C,
Campagna D, Vitulo N, Valle G, Nelson DR, De
Paoli E, Szinay D, de Jong HH, Bai YL, Visser RGF,
Lankhorst RMK, Beasley H, McLaren K, Nicholson C,
Riddle C, Gianese G, Consortium TG (2012) The
tomato genome sequence provides insights into fleshy
fruit evolution. Nature 485(7400):635–641
Stomp AM (2005) The duckweeds: a valuable plant for
biomanufacturing. Biotechnol Annu Rev 11:69–99
Vunsh R, Li J, Hanania U, Edelman M, Flaishman M,
Perl A, Wisniewski JP, Freyssinet G (2007) High
expression of transgene protein in Spirodela. Plant
Cell Rep 26(9):1511–1519
Wang W (1990) Literature review on duckweed toxicity
testing. Environ Res 52(1):7–22
Wang W, Kerstetter R, Michael T (2011) Evolution of
genome size in duckweeds (Lemnaceae). J Bot 2:1
Wang W, Haberer G, Gundlach H, Glasser C, Nussbaumer T, Luo MC, Lomsadze A, Borodovsky M,
Kerstetter RA, Shanklin J, Byrant DW, Mockler TC,
Appenroth KJ, Grimwood J, Jenkins J, Chow J,
Choi C, Adam C, Cao XH, Fuchs J, Schubert I,
Rokhsar D, Schmutz J, Michael TP, Mayer KFX,
Messing J (2014) The Spirodela polyrhiza genome
reveals insights into its neotenous reduction fast
growth and aquatic lifestyle. Nat Commun, 5
Wang WQ, Wu YR, Yan YH, Ermakova M, Kerstetter R,
Messing J (2010) DNA barcoding of the Lemnaceae, a
family of aquatic monocots. BMC Plant Biol 10
84
D. An and W. Wang
inhibition test (ISO 20079) with Lemna minor L.
clone St. J Plant Physiol 164(12):1656–1664
Olah V, Hepp A, Meszaros I (2016) Assessment of giant
duckweed (Spirodela polyrhiza L. Schleiden) turions
as model objects in ecotoxicological applications. Bull
Environ Contam Toxicol 96(5):596–601
Ozengin N, Elmaci A (2007) Performance of duckweed
(Lemna minor L.) on different types of wastewater
treatment. J Environ Biol 28(2):307–314
Rahman MA, Hasegawa H (2011) Aquatic arsenic:
phytoremediation using floating macrophytes. Chemosphere 83(5):633–646
Rai UN, Sinha S, Tripathi RD, Chandra P (1995)
Waste-water treatability potential of some aquatic
macrophytes—removal of heavy-metals. Ecol Eng 5
(1):5–12
Sato S, Tabata S, Hirakawa H, Asamizu E, Shirasawa K,
Isobe S, Kaneko T, Nakamura Y, Shibata D, Aoki K,
Egholm M, Knight J, Bogden R, Li CB, Shuang Y,
Xu X, Pan SK, Cheng SF, Liu X, Ren YY, Wang J,
Albiero A, Dal Pero F, Todesco S, Van Eck J,
Buels RM, Bombarely A, Gosselin JR, Huang MY,
Leto JA, Menda N, Strickler S, Mao LY, Gao S,
Tecle IY, York T, Zheng Y, Vrebalov JT, Lee J,
Zhong SL, Mueller LA, Stiekema WJ, Ribeca P,
Alioto T, Yang WC, Huang SW, Du YC, Zhang ZH,
Gao JC, Guo YM, Wang XX, Li Y, He J, Li CY,
Cheng ZK, Zuo JR, Ren JF, Zhao JH, Yan LH,
Jiang HL, Wang B, Li HS, Li ZJ, Fu FY, Chen BT,
Han B, Feng Q, Fan DL, Wang Y, Ling HQ,
Xue YBA, Ware D, McCombie WR, Lippman ZB,
Chia JM, Jiang K, Pasternak S, Gelley L, Kramer M,
Anderson LK, Chang SB, Royer SM, Shearer LA,
Stack SM, Rose JKC, Xu YM, Eannetta N, Matas AJ,
McQuinn R, Tanksley SD, Camara F, Guigo R,
Rombauts S, Fawcett J, Van de Peer Y, Zamir D,
Liang CB, Spannagl M, Gundlach H, Bruggmann R,
Mayer K, Jia ZQ, Zhang JH, Ye ZBA, Bishop GJ,
Butcher S, Lopez-Cobollo R, Buchan D, Filippis I,
Abbott J, Dixit R, Singh M, Singh A, Pal JK, Pandit A,
Singh PK, Mahato AK, Dogra V, Gaikwad K,
Sharma TR, Mohapatra T, Singh NK, Causse M,
Rothan C, Schiex T, Noirot C, Bellec A, Klopp C,
Delalande C, Berges H, Mariette J, Frasse P,
Vautrin S, Zouine M, Latche A, Rousseau C,
Regad F, Pech JC, Philippot M, Bouzayen M, Pericard P, Osorio S, del Carmen AF, Monforte A,
Granell A, Fernandez-Munoz R, Conte M, Lichtenstein G, Carrari F, De Bellis G, Fuligni F, Peano C,
Grandillo S, Termolino P, Pietrella M, Fantini E,
Falcone G, Fiore A, Giuliano G, Lopez L, Facella P,
Perrotta G, Daddiego L, Bryan G, Orozco M, Pastor X,
Torrents D, van Schriek KNVMGM, Feron RMC, van
Oeveren J, de Heer P, daPonte L, Jacobs-Oomen S,
Cariaso M, Prins M, van Eijk MJT, Janssen A, van
Haaren MJJ, Jo SH, Kim J, Kwon SY, Kim S,
Koo DH, Lee S, Hur CG, Clouser C, Rico A,
Hallab A, Gebhardt C, Klee K, Jocker A, Warfsmann J, Gobel U, Kawamura S, Yano K, Sherman JD,
Fukuoka H, Negoro S, Bhutty S, Chowdhury P,
Chattopadhyay D, Datema E, Smit S, Schijlen EWM,
van de Belt J, van Haarst JC, Peters SA, van
Staveren MJ, Henkens MHC, Mooyman PJW, Hesselink T, van Ham RCHJ, Jiang GY, Droege M,
Choi D, Kang BC, Kim BD, Park M, Kim S, Yeom SI,
Lee YH, Choi YD, Li GC, Gao JW, Liu YS,
Huang SX, Fernandez-Pedrosa V, Collado C,
Zuniga S, Wang GP, Cade R, Dietrich RA,
Rogers J, Knapp S, Fei ZJ, White RA,
Thannhauser TW, Giovannoni JJ, Botella MA, Gilbert L, Gonzalez R, Goicoechea JL, Yu Y, Kudrna D,
Collura K, Wissotski M, Wing R, Schoof H, Meyers BC, Gurazada AB, Green PJ, Mathur S, Vyas S,
Solanke AU, Kumar R, Gupta V, Sharma AK,
Khurana P, Khurana JP, Tyagi AK, Dalmay T,
Mohorianu I, Walts B, Chamala S, Barbazuk WB,
Li JP, Guo H, Lee TH, Wang YP, Zhang D,
Paterson AH, Wang XY, Tang HB, Barone A,
Chiusano ML, Ercolano MR, D’Agostino N, Di
Filippo M, Traini A, Sanseverino W, Frusciante L,
Seymour GB, Elharam M, Fu Y, Hua A, Kenton S,
Lewis J, Lin SP, Najar F, Lai HS, Qin BF, Qu CM,
Shi RH, White D, White J, Xing YB, Yang KQ, Yi J,
Yao ZY, Zhou LP, Roe BA, Vezzi A, D’Angelo M,
Zimbello R, Schiavon R, Caniato E, Rigobello C,
Campagna D, Vitulo N, Valle G, Nelson DR, De
Paoli E, Szinay D, de Jong HH, Bai YL, Visser RGF,
Lankhorst RMK, Beasley H, McLaren K, Nicholson C,
Riddle C, Gianese G, Consortium TG (2012) The
tomato genome sequence provides insights into fleshy
fruit evolution. Nature 485(7400):635–641
Stomp AM (2005) The duckweeds: a valuable plant for
biomanufacturing. Biotechnol Annu Rev 11:69–99
Vunsh R, Li J, Hanania U, Edelman M, Flaishman M,
Perl A, Wisniewski JP, Freyssinet G (2007) High
expression of transgene protein in Spirodela. Plant
Cell Rep 26(9):1511–1519
Wang W (1990) Literature review on duckweed toxicity
testing. Environ Res 52(1):7–22
Wang W, Kerstetter R, Michael T (2011) Evolution of
genome size in duckweeds (Lemnaceae). J Bot 2:1
Wang W, Haberer G, Gundlach H, Glasser C, Nussbaumer T, Luo MC, Lomsadze A, Borodovsky M,
Kerstetter RA, Shanklin J, Byrant DW, Mockler TC,
Appenroth KJ, Grimwood J, Jenkins J, Chow J,
Choi C, Adam C, Cao XH, Fuchs J, Schubert I,
Rokhsar D, Schmutz J, Michael TP, Mayer KFX,
Messing J (2014) The Spirodela polyrhiza genome
reveals insights into its neotenous reduction fast
growth and aquatic lifestyle. Nat Commun, 5
Wang WQ, Wu YR, Yan YH, Ermakova M, Kerstetter R,
Messing J (2010) DNA barcoding of the Lemnaceae, a
family of aquatic monocots. BMC Plant Biol 10
84
D. An and W. Wang