Table 10.2 Sequencing and features of duckweed chloroplast genomes
Species
DNA
preparation
Purification
Sequence Assembly Annotation
Genbank
number
Genome
size (bp)
IR
size
(bp)
Protein
coding
gene
tRNA rRNA References
Lemna
minor
Total DNA Long PCR
amplification
Sanger
Gene
studio
program
BLAST;
tRNAscan-SE
program;
DOGMA
DQ400350 165,955
31,223 78
30
4
Mardanov et al.
2008
Spirodela
polyrhiza
7498
Total DNA Computational
filtration
NGS
SOLiD
system de
novo
accessory
tools and
velvet
assembly
engine
BLAST;
tRNAscan-SE
program;
DOGMA
JN160603 168,788
31,755 85
37
8
Wang et al. (2011)
Wolffiella
lingulata
7289
JN160604 169,337
31,683 83
37
8
Wolffia
australiana
7733
JN160605 168,704
31,930 83
37
8
108
Y. Zhang and W. Wang