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48. K.D. Phend, A. Rustioni, R.J. Weinberg, An osmium-free method of epon embedment that
preserves both ultrastructure and antigenicity for post-embedding immunocytochemistry.
J. Histochem. Cytochem. 43, 283–292 (1995)
49. A. Sawaguchi, S. Ide, Y. Goto, J. Kawano, T. Oinuma, T. Suganuma, A simple contrast
enhancement by potassium permanganate oxidation for Lowicryl K4 M ultrathin sections
prepared by high pressure freezing/freeze substitution. J. Microsc. 201, 77–83 (2001)
50. J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch,
C. Rueden, S. Saalfeld, B. Schmid, J.Y. Tinevez, D.J. White, V. Hartenstein, K. Eliceiri,
P. Tomancak, A. Cardona, Fiji: an open-source platform for biological-image analysis. Nat.
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51. A. Cardona, S. Saalfeld, J. Schindelin, I. Arganda-Carreras, S. Preibisch, M. Longair,
P. Tomancak, V. Hartenstein, R.J. Douglas, TrakEM2 software for neural circuit reconstruction. PLoS One 7(6), e38011 (2012)
52. S. Saalfeld, R. Fetter, A. Cardona, P. Tomancak, Elastic volume reconstruction from series of
ultra-thin microscopy sections. Nat. Methods 9, 717–720 (2012)
6 Conjugate Immunofluorescence—SEM Array Tomography …
181
630–646 (1995)
33. A. Matsubara, J.H. Laake, S. Davanger, S. Usami, O.P. Ottersen, Organization of AMPA
receptor subunits at a glutamate synapse: a quantitative immunogold analysis of hair cell
synapses in the rat organ of Corti. J. Neurosci. 16, 4457–4467 (1996)
34. K.W. Roche, C.D. Ly, R.S. Petralia, Y.X. Wang, A.W. McGee, D.S. Bredt, R.J. Wenthold,
Postsynaptic density-93 interacts with the delta2 glutamate receptor subunit at parallel fiber
synapses. J. Neurosci. 19, 3926–3934 (1999)
35. L.H. Bergersen, J. Storm-Mathisen, V. Gundersen, Immunogold quantification of amino acids
and proteins in complex subcellular compartments. Nat. Protoc. 3, 144–152 (2008)
36. A.L. Jacob, R.J. Weinberg, The organization of AMPA receptor subunits at the postsynaptic
membrane. Hippocampus 25, 798–812 (2015)
37. J.B. Pawley, Handbook of Biological Confocal Microscopy (Plenum Press, New York, 1995)
38. R. Heintzmann, C. Cremer, Lateral modulated excitation microscopy: improvement of
resolution by using a diffraction grating. Proc. SPIE 3568, 185–196 (1999)
39. M.G.L. Gustafsson, Surpassing the lateral resolution limit by a factor of two using structured
illumination microscopy. J. Microsc. 198, 82–87 (2000)
40. W.J. Wang, H.G. Tay, R. Soni, G.S. Perumal, M.G. Goll, F.P. Macaluso, J.M. Asara, J.D.
Amack, M.F. Tsou, CEP162 is an axoneme-recognition protein promoting ciliary transition
zone assembly at the cilia base. Nat. Cell Biol. 15(6), 591–601 (2013)
41. C.J. Peddie, K. Blight, E. Wilson, C. Melia, J. Marrison, R. Carzaniga, M.C. Domart,
P. O’Toole, B. Larijani, L.M. Collinson, Correlative and integrated light and electron
microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells.
Ultramicroscopy 143, 3–14 (2014)
42. P. Somogyi, A.J. Hodgson, I.W. Chubb, B. Penke, A. Erdei, Antisera to gamma-aminobutyric
acid. II. Immunocytochemical application to the central nervous system. J. Histochem.
Cytochem. 33, 240–248 (1985)
43. O.P. Ottersen, J. Storm-Mathiesen, Localization of amino acid neurotransmitters by
immunocytochemistry. Trends Neurosci. 10, 250–255 (1987)
44. K. Weber, P.C. Rathke, M. Osborn, Cytoplasmic microtubular images in glutaraldehyde-fixed
tissue culture cells by electron microscopy and by immunofluorescence microscopy. Proc.
Natl. Acad. Sci. U S A 75(4), 1820–1824 (1978)
45. M.C. Willingham, An alternative fixation-processing method for preembedding ultrastructural
immunocytochemistry of cytoplasmic antigens: the GBS (glutaraldehyde-borohydridesaponin) procedure. J. Histochem. Cytochem. 31(6), 791–798 (1983)
46. J.A. Terzakis, Uranyl acetate, a stain and a fixative. J. Ultrastruct. Res. 22, 168–184 (1968)
47. M.A. Berryman, R.D. Rodewald, An enhanced method for post-embedding immunocytochemical staining which preserves cell membranes. J. Histochem. Cytochem. 38, 159–170
(1990)
48. K.D. Phend, A. Rustioni, R.J. Weinberg, An osmium-free method of epon embedment that
preserves both ultrastructure and antigenicity for post-embedding immunocytochemistry.
J. Histochem. Cytochem. 43, 283–292 (1995)
49. A. Sawaguchi, S. Ide, Y. Goto, J. Kawano, T. Oinuma, T. Suganuma, A simple contrast
enhancement by potassium permanganate oxidation for Lowicryl K4 M ultrathin sections
prepared by high pressure freezing/freeze substitution. J. Microsc. 201, 77–83 (2001)
50. J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch,
C. Rueden, S. Saalfeld, B. Schmid, J.Y. Tinevez, D.J. White, V. Hartenstein, K. Eliceiri,
P. Tomancak, A. Cardona, Fiji: an open-source platform for biological-image analysis. Nat.
Methods 9, 676–682 (2012)
51. A. Cardona, S. Saalfeld, J. Schindelin, I. Arganda-Carreras, S. Preibisch, M. Longair,
P. Tomancak, V. Hartenstein, R.J. Douglas, TrakEM2 software for neural circuit reconstruction. PLoS One 7(6), e38011 (2012)
52. S. Saalfeld, R. Fetter, A. Cardona, P. Tomancak, Elastic volume reconstruction from series of
ultra-thin microscopy sections. Nat. Methods 9, 717–720 (2012)
6 Conjugate Immunofluorescence—SEM Array Tomography …
181