13. R. Jungmann, C. Steinhauer, M. Scheible, A. Kuzyk, P. Tinnefeld, F.C. Simmel,
Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of
transient binding on DNA origami. NanoLett 10, 4756–4761 (2010)
14. T. Kiuchi, M. Higuchi, A. Takamura, M. Maruoka, N. Watanabe, Multitarget super-resolution
microscopy with high-density labeling by exchangeable probes. Nat. Methods 12, 743–746
(2015)
15. J.R. Anderson, B.W. Jones, J.-H. Yang, M.V. Shaw, C.B. Watt, P. Koshevoy, J. Spaltenstein,
E. Jurrus, U.V. Kannan, R. Whitaker, D. Mastronarde, T. Tasdizen, R. Marc, A computational
framework for ultrastructural mapping of neural circuitry. PLoS Biol. 7(3), e1000074 (2009)
16. J.R. Anderson, B.W. Jones, C.B. Watt, M.V. Shaw, J.H. Yang, D. Demill, J.S. Lauritzen, Y.
Lin, K.D. Rapp, D. Mastronarde, P. Koshevoy, B. Grimm, T. Tasdizen, R. Whitaker, R.E.
Marc, Exploring the retinal connectome. Mol. Vis. 17, 355–379 (2011)
17. D. Oberti, M.A. Kirschmann, R.H. Hahnloser, Projection neuron circuits resolved using
correlative array tomography. Front Neurosci. 5, 50 (2011)
18. F. Collman, J. Buchanan, K.D. Phend, K.D. Micheva, R.J. Weinberg, S.J. Smith, Mapping
synapses by conjugate light-electron array tomography. J. Neurosci. 35(14), 5792–5807
(2015)
19. E.B. Bloss, M.S. Cembrowski, B. Karsh, J. Colonell, R.D. Fetter, N. Spruston, Structured
dendritic inhibition supports branch-selective integration in CA1 pyramidal cells. Neuron 89
(5), 1016–1030 (2016)
20. R. Shahidi, E.A. Williams, M. Conzelmann, A. Asadulina, C. Verasztó, S. Jasek, L.A.
Bezares-Calderón, G. Jékely, A serial multiplex immunogold labeling method for identifying
peptidergic neurons in connectomes. Elife 4, e11147 (2015)
21. J. Storm-Mathisen, A.K. Leknes, A.T. Bore, J.L. Vaaland, P. Edminson, F.M. Haug, O.
P. Ottersen, First visualization of glutamate and GABA in neurones by immunocytochemistry. Nature 301(5900), 517–520 (1983)
22. P. Somogyi, A.J. Hodgson, Antisera to gamma-aminobutyric acid. III. Demonstration
of GABA in Golgi-impregnated neurons and in conventional electron microscopic sections of
cat striate cortex. J. Histochem. Cytochem. 33(3), 249–257 (1985)
23. R.E. Marc, B.W. Jones, C.B. Watt, J.R. Anderson, C. Sigulinsky, S. Lauritzen, Retinal
connectomics: towards complete, accurate networks. Prog Retin Eye Res 37, 141–162 (2013)
24. G. Pelletier, R. Puviani, O. Bosler, L. Descarries, Immunocytochemical detection of peptides
in osmicated and plastic-embedded tissue. An electron microscopic study. J. Histochem.
Cytochem. 29(6), 759–764 (1981)
25. A. Merighi, F. Cruz, A. Coimbra, Immunocytochemical staining of neuropeptides in terminal
arborization of primary afferent fibers anterogradely labeled and identified at light and
electron microscopic levels. J. Neurosci. Methods 42(1–2), 105–113 (1992)
26. A.J. Nielson, W.P. Griffith, Tissue fixation by osmium tetroxide. A possible role for proteins.
J. Histochem. Cytochem. 27(5), 997–999 (1979)
27. B.E. Causton, Does the embedding chemistry interact with tissue? in The Science of
Biological Specimen Preparation 1985, ed. by M. Müller, R.P. Becker, A. Boyde, J.
J. Wolosewick (SEM Inc, AMF O’Hare, Chicago), pp. 209–214
28. S.H. Brorson, F. Skjørten, The theoretical relationship of immunogold labeling on acrylic
sections and epoxy sections. Micron 27(3–4), 193–201 (1996)
29. G.R. Newman, J.A. Hobot, Modern acrylics for post-embedding immunostaining techniques.
J. Histochem. Cytochem. 35(9), 971–981 (1987)
30. E. Kellenberger, The Response of Biological Macromolecules and Supramolecular Structures
to the Physics of Specimen Cryopreparation, in Cryotechniques in Biological Electron
Microscopy, ed. by R.A. Steinbrecht, K. Zierold (Springer, Heidelberg, 1987), pp. 35–63
31. A. Baude, Z. Nusser, E. Molnár, R.A. McIlhinney, P. Somogyi, High-resolution immunogold
localization of AMPA type glutamate receptor subunits at synaptic and non-synaptic sites in
rat hippocampus. Neuroscience 69, 1031–1055 (1995)
32. Z. Nusser, J.D. Roberts, A. Baude, J.G. Richards, W. Sieghart, P. Somogyi,
Immunocytochemical localization of the alpha 1 and beta 2/3 subunits of the GABAA
180
K. D. Micheva and K. D. Phend
Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of
transient binding on DNA origami. NanoLett 10, 4756–4761 (2010)
14. T. Kiuchi, M. Higuchi, A. Takamura, M. Maruoka, N. Watanabe, Multitarget super-resolution
microscopy with high-density labeling by exchangeable probes. Nat. Methods 12, 743–746
(2015)
15. J.R. Anderson, B.W. Jones, J.-H. Yang, M.V. Shaw, C.B. Watt, P. Koshevoy, J. Spaltenstein,
E. Jurrus, U.V. Kannan, R. Whitaker, D. Mastronarde, T. Tasdizen, R. Marc, A computational
framework for ultrastructural mapping of neural circuitry. PLoS Biol. 7(3), e1000074 (2009)
16. J.R. Anderson, B.W. Jones, C.B. Watt, M.V. Shaw, J.H. Yang, D. Demill, J.S. Lauritzen, Y.
Lin, K.D. Rapp, D. Mastronarde, P. Koshevoy, B. Grimm, T. Tasdizen, R. Whitaker, R.E.
Marc, Exploring the retinal connectome. Mol. Vis. 17, 355–379 (2011)
17. D. Oberti, M.A. Kirschmann, R.H. Hahnloser, Projection neuron circuits resolved using
correlative array tomography. Front Neurosci. 5, 50 (2011)
18. F. Collman, J. Buchanan, K.D. Phend, K.D. Micheva, R.J. Weinberg, S.J. Smith, Mapping
synapses by conjugate light-electron array tomography. J. Neurosci. 35(14), 5792–5807
(2015)
19. E.B. Bloss, M.S. Cembrowski, B. Karsh, J. Colonell, R.D. Fetter, N. Spruston, Structured
dendritic inhibition supports branch-selective integration in CA1 pyramidal cells. Neuron 89
(5), 1016–1030 (2016)
20. R. Shahidi, E.A. Williams, M. Conzelmann, A. Asadulina, C. Verasztó, S. Jasek, L.A.
Bezares-Calderón, G. Jékely, A serial multiplex immunogold labeling method for identifying
peptidergic neurons in connectomes. Elife 4, e11147 (2015)
21. J. Storm-Mathisen, A.K. Leknes, A.T. Bore, J.L. Vaaland, P. Edminson, F.M. Haug, O.
P. Ottersen, First visualization of glutamate and GABA in neurones by immunocytochemistry. Nature 301(5900), 517–520 (1983)
22. P. Somogyi, A.J. Hodgson, Antisera to gamma-aminobutyric acid. III. Demonstration
of GABA in Golgi-impregnated neurons and in conventional electron microscopic sections of
cat striate cortex. J. Histochem. Cytochem. 33(3), 249–257 (1985)
23. R.E. Marc, B.W. Jones, C.B. Watt, J.R. Anderson, C. Sigulinsky, S. Lauritzen, Retinal
connectomics: towards complete, accurate networks. Prog Retin Eye Res 37, 141–162 (2013)
24. G. Pelletier, R. Puviani, O. Bosler, L. Descarries, Immunocytochemical detection of peptides
in osmicated and plastic-embedded tissue. An electron microscopic study. J. Histochem.
Cytochem. 29(6), 759–764 (1981)
25. A. Merighi, F. Cruz, A. Coimbra, Immunocytochemical staining of neuropeptides in terminal
arborization of primary afferent fibers anterogradely labeled and identified at light and
electron microscopic levels. J. Neurosci. Methods 42(1–2), 105–113 (1992)
26. A.J. Nielson, W.P. Griffith, Tissue fixation by osmium tetroxide. A possible role for proteins.
J. Histochem. Cytochem. 27(5), 997–999 (1979)
27. B.E. Causton, Does the embedding chemistry interact with tissue? in The Science of
Biological Specimen Preparation 1985, ed. by M. Müller, R.P. Becker, A. Boyde, J.
J. Wolosewick (SEM Inc, AMF O’Hare, Chicago), pp. 209–214
28. S.H. Brorson, F. Skjørten, The theoretical relationship of immunogold labeling on acrylic
sections and epoxy sections. Micron 27(3–4), 193–201 (1996)
29. G.R. Newman, J.A. Hobot, Modern acrylics for post-embedding immunostaining techniques.
J. Histochem. Cytochem. 35(9), 971–981 (1987)
30. E. Kellenberger, The Response of Biological Macromolecules and Supramolecular Structures
to the Physics of Specimen Cryopreparation, in Cryotechniques in Biological Electron
Microscopy, ed. by R.A. Steinbrecht, K. Zierold (Springer, Heidelberg, 1987), pp. 35–63
31. A. Baude, Z. Nusser, E. Molnár, R.A. McIlhinney, P. Somogyi, High-resolution immunogold
localization of AMPA type glutamate receptor subunits at synaptic and non-synaptic sites in
rat hippocampus. Neuroscience 69, 1031–1055 (1995)
32. Z. Nusser, J.D. Roberts, A. Baude, J.G. Richards, W. Sieghart, P. Somogyi,
Immunocytochemical localization of the alpha 1 and beta 2/3 subunits of the GABAA
180
K. D. Micheva and K. D. Phend