Table 6.1 Antibodies for conjugate IF-SEM array tomography
Antigen
Host
Antibody source
RRID
Neurotransmitters
GABA
Guinea pig Millipore AB175
RRID:AB_91011
GABA
Rabbit
Millipore AB131
RRID:AB_2278931
Glutamate
Rabbit
Millipore AB5018
RRID:AB_91640
Synaptic
Synapsin
Rabbit
Cell Signaling 5297
RRID:AB_2616578
Synaptophysin
Mouse
Abcam ab8049
RRID:AB_2198854
PSD95
Rabbit
Cell Signaling 3450
RRID:AB_2292883
PSD95
Mouse
NeuroMab 75-028
RRID:AB_2292909
VGluT1
Guinea pig Millipore AB5905
RRID:AB_2301751
VGluT2
Rabbit
Synaptic systems 135 403
RRID:AB_887883
GAD2
Rabbit
Cell signaling 5843
RRID:AB_10835855
Gephyrin
Mouse
NeuroMab 73-465
RRID:AB_2632414
Clathrin
Rabbit
Cell signaling 4796
RRID:AB_10557412
Cell type markers
Parvalbumin
Rabbit
SWANT PV28
RRID:AB_10013386
Calbindin
Rabbit
Millipore AB1778
RRID:AB_2068336
Calbindin
Mouse
NeuroMab 73-448
RRID:AB_2619740
NPY
Mouse
NeuroMab 73-456
RRID:AB_2629421
Tyrosine hydroxylase
Rabbit
Millipore AB152
RRID:AB_390204
Receptors
GluA2/3
Rabbit
Millipore AB1506
RRID:AB_90710
GluN1
Mouse
Millipore MAB363
RRID:AB_94946
GluN2B
Mouse
NeuroMab 75-101
RRID:AB_2232584
GABA A Receptor b2/3
Mouse
Millipore MAB341
RRID:AB_2109419
Cytoskeletal
a Tubulin
Rabbit
Abcam ab18251
RRID:AB_2210057
Acetylated a tubulin
Mouse
Sigma T6793
RRID:AB_477585
bIII Tubulin
Chicken
Abcam ab41489
RRID:AB_727049
Detyrosinated tubulin
Rabbit
Millipore AB3201
RRID:AB_177350
d2 Tubulin
Rabbit
Millipore AB3203
RRID:AB_177351
Neurofilament 200
Rabbit
Sigma N4142
RRID:AB_477272
Neurofilament, heavy chain
Chicken
AVES NF-H
RRID:AB_2313552
Neurofilament, medium chain Chicken
AVES NF-M
RRID:AB_2313554
Neurofilament, light chain
Chicken
AVES NF-L
RRID:AB_2313553
c actin
Mouse
Sigma A8481
RRID:AB_2289264
Synaptopodin
Rabbit
Synaptic Systems 163 002 RRID:AB_887825
Glial
Glutamine synthetase
Mouse
BD Biosciences 610517
RRID:AB_397879
GFAP
Chicken
AVES GFAP
RRID:AB_2313547
(continued)
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