121. Zhao Q, Li XF, Le XC (2008) Aptamer-modified monolithic capillary chromatography for
protein separation and detection. Anal Chem 80:3915–3920
122. Kuehne C, Wedepohl S, Dernedde J (2017) Single-step purification of monomeric l-selectin
via aptamer affinity chromatography. Sensors 17:226–232
123. Finlay TH, Troll V, Levy M, Johnson AJ, Hodgins LT (1978) New methods for the preparation of biospecific adsorbents and immobilized enzymes utilizing trichloro-s-triazine. Anal
Biochem 87:77–90
124. Walter JG, Kökpinar O, Friehs K, Stahl F, Scheper T (2008) Systematic investigation
of optimal aptamer immobilization for protein-microarray applications. Anal Chem
80:7372–7378
125. Kokpinar O, Walter JG, Shoham Y, Stahl F, Scheper T (2011) Aptamer-based downstream
processing of his-tagged proteins utilizing magnetic beads. Biotechnol Bioeng 108:2371–2379
126. Ferguson JA, Boles TC, Adams CP, Walt DR (1996) A fiber-optic DNA biosensor microarray
for the analysis of gene expression. Nat Biotechnol 14:1681–1684
127. Weston PD, Avrameas S (1971) Proteins coupled to polyacrylamide beads using glutaraldehyde. Biochem Biophys Res Commun 45:1574–1580
128. Cambiaso CL, Goffinet A, Vaerman JP, Heremans JF (1975) Glutaraldehyde-activated
aminohexyl- derivative of Sepharose 4B as a new versatile immunoabsorbent. Immunochemistry 12:273–278
129. Oktem HA, Bayramoglu G, Ozalp VC, Arica MY (2007) Single-step purification of recombinant Thermus aquaticus DNA polymerase using DNA-aptamer immobilized novel affinity
magnetic beads. Biotechnol Prog 23:146–154
130. Han B, Zhao C, Yin J, Wang H (2012) High performance aptamer affinity chromatography for
single step selective extraction and screening of basic protein lysozyme. J Chromatogr B
903:112–117
131. Hemminki K, Suni R (1984) Sites of reaction of glutaraldehyde and acetaldehyde with
nucleosides. Arch Toxicol 55:186–190
132. Chockalingam PS, Gadgil H, Jarrett HW (2002) DNA-support coupling for transcription factor
purification. Comparison of aldehyde, cyanogen bromide and N-hydroxysuccinimide chemistries. J Chromatogr A 942:167–175
133. Stage DE, Mannik M (1974) Covalent binding of molecules to CNBr-activated agarose:
parameters relevant to the activation and coupling reactions. Biochim Biophys Acta
343:382–391
134. Kohn J, Wilchek M (1982) A new approach (cyano-transfer) for cyanogen bromide activation
of Sepharose at neutral pH, which yields activated resins, free of interfering nitrogen derivatives. Biochem Biophys Res Commun 107:878–884
135. Benes MJ, Adamkova K, Turkova J (1991) Activation of beaded cellulose with 2,4,6trichlorotriazine. Bioact Compat Polym 6:406–413
136. Kawamura K, Okamoto F (2000) Condensation reaction of hexanucleotides containing guanine and cytosine with water soluble carbodiimide. Nucleic Acids Symp Ser 44:217–218
137. Miyakawa S, Nomura Y, Sakamoto T, Yamaguchi Y, Kato K, Yamazaki S, Nakamura Y
(2008) Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G. RNA 14:1154–1163
138. Ahirwar R, Nahar P (2015) Development of an aptamer-affinity chromatography for efficient
single step purification of Concanavalin A from Canavalia ensiformis. J Chromatogr B
997:105–109
139. Frost RG, Monthony JF, Engelhorn SC, Siebert CJ (1981) Covalent immobilization of proteins to N-hydroxysuccinimide ester derivatives of agarose. Effect of protein charge on
immobilization. Biochim Biophys Acta 670:163–169
140. Boschetti E, Perret G (2011) Patent Application WO/2012/090183
141. Sherbet GV, Lakshmi MS, Cajone F (1983) Isoelectric characteristics and the secondary
structure of some nucleic acids. Biophys Struct Mech 10:121–128
136
G. Perret and E. Boschetti
protein separation and detection. Anal Chem 80:3915–3920
122. Kuehne C, Wedepohl S, Dernedde J (2017) Single-step purification of monomeric l-selectin
via aptamer affinity chromatography. Sensors 17:226–232
123. Finlay TH, Troll V, Levy M, Johnson AJ, Hodgins LT (1978) New methods for the preparation of biospecific adsorbents and immobilized enzymes utilizing trichloro-s-triazine. Anal
Biochem 87:77–90
124. Walter JG, Kökpinar O, Friehs K, Stahl F, Scheper T (2008) Systematic investigation
of optimal aptamer immobilization for protein-microarray applications. Anal Chem
80:7372–7378
125. Kokpinar O, Walter JG, Shoham Y, Stahl F, Scheper T (2011) Aptamer-based downstream
processing of his-tagged proteins utilizing magnetic beads. Biotechnol Bioeng 108:2371–2379
126. Ferguson JA, Boles TC, Adams CP, Walt DR (1996) A fiber-optic DNA biosensor microarray
for the analysis of gene expression. Nat Biotechnol 14:1681–1684
127. Weston PD, Avrameas S (1971) Proteins coupled to polyacrylamide beads using glutaraldehyde. Biochem Biophys Res Commun 45:1574–1580
128. Cambiaso CL, Goffinet A, Vaerman JP, Heremans JF (1975) Glutaraldehyde-activated
aminohexyl- derivative of Sepharose 4B as a new versatile immunoabsorbent. Immunochemistry 12:273–278
129. Oktem HA, Bayramoglu G, Ozalp VC, Arica MY (2007) Single-step purification of recombinant Thermus aquaticus DNA polymerase using DNA-aptamer immobilized novel affinity
magnetic beads. Biotechnol Prog 23:146–154
130. Han B, Zhao C, Yin J, Wang H (2012) High performance aptamer affinity chromatography for
single step selective extraction and screening of basic protein lysozyme. J Chromatogr B
903:112–117
131. Hemminki K, Suni R (1984) Sites of reaction of glutaraldehyde and acetaldehyde with
nucleosides. Arch Toxicol 55:186–190
132. Chockalingam PS, Gadgil H, Jarrett HW (2002) DNA-support coupling for transcription factor
purification. Comparison of aldehyde, cyanogen bromide and N-hydroxysuccinimide chemistries. J Chromatogr A 942:167–175
133. Stage DE, Mannik M (1974) Covalent binding of molecules to CNBr-activated agarose:
parameters relevant to the activation and coupling reactions. Biochim Biophys Acta
343:382–391
134. Kohn J, Wilchek M (1982) A new approach (cyano-transfer) for cyanogen bromide activation
of Sepharose at neutral pH, which yields activated resins, free of interfering nitrogen derivatives. Biochem Biophys Res Commun 107:878–884
135. Benes MJ, Adamkova K, Turkova J (1991) Activation of beaded cellulose with 2,4,6trichlorotriazine. Bioact Compat Polym 6:406–413
136. Kawamura K, Okamoto F (2000) Condensation reaction of hexanucleotides containing guanine and cytosine with water soluble carbodiimide. Nucleic Acids Symp Ser 44:217–218
137. Miyakawa S, Nomura Y, Sakamoto T, Yamaguchi Y, Kato K, Yamazaki S, Nakamura Y
(2008) Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G. RNA 14:1154–1163
138. Ahirwar R, Nahar P (2015) Development of an aptamer-affinity chromatography for efficient
single step purification of Concanavalin A from Canavalia ensiformis. J Chromatogr B
997:105–109
139. Frost RG, Monthony JF, Engelhorn SC, Siebert CJ (1981) Covalent immobilization of proteins to N-hydroxysuccinimide ester derivatives of agarose. Effect of protein charge on
immobilization. Biochim Biophys Acta 670:163–169
140. Boschetti E, Perret G (2011) Patent Application WO/2012/090183
141. Sherbet GV, Lakshmi MS, Cajone F (1983) Isoelectric characteristics and the secondary
structure of some nucleic acids. Biophys Struct Mech 10:121–128
136
G. Perret and E. Boschetti