91
Table 6.2 Different types of biofactories for pectinase production commonly used for industrial
production microorganisms
Strain
Purification method
Recovery Purification stage
Aspergillus fumigatus
CM-Sephadex C-50 and
Sephacryl S-200
26.1%
Enzyme pectinase with
4.45-fold
(Okinji et al. 2019)
Aspergillus kawachii
Acetone precipitation
8.6%
Enzyme PGI with
470-fold
Contreas and Voget
(2004)
Sepharose Q
Sephacryl S-100 column
chromatography
Aspergillus niger MTCC
478
Acetone precipitation
60–90%
Enzyme PG with
15.28-fold
Anand et al. (2017)
CM cellulose column
chromatography
Aspergillus flavus
Ammonium sulfate fraction 10.3%
Enzyme PL with 58-fold
Celestino et al. (2006)
DEAE-cellulose ion
exchange
Sephadex G-100 gel filtration
Aspergillus japonicus
Hydrophobic
–
Enzyme PGI, PG11, PE1,
PE11, PL with range
2–20-fold
Semenova et al. (2003)
Ion exchange column
chromatography
Aspergillus niger ANO7 Anion exchange
chromatography on
DEAE-cellulose
52.6%
Enzyme PG with
24.8-fold
Patidar et al. (2017)
Gel filtration
chromatography using
Sephadex G-200
Aspergillus flavus
Ethanol precipitation
3%
Enzyme PG with
9.93-fold
Doughari and
Onyebarachi (2019)
Sephadex G-75
Aspergillus awamori L1 Ethanol precipitation
68.6%
Enzyme PG with
30.4-fold
Ngo et al. (2008)
Sephadex G-75 gel filtration
Thermoascus
aurantiacus
Sephadex G-75 gel filtration 24.6%
Enzyme PG with 21-fold
Martins et al. (2007)
SP-Sepharose ion exchange
chromatography
Acrophialophora
nainiana
Sephacryl S-100 gel
filtration
60.6%
Enzyme PG and PL with
9.37-fold
Celestino et al. (2006)
DEAE-Sepharose ion
exchange
Sephadex FG-50
Streptomyces lydicus
Ultrafiltration
54.6%
Enzyme PG with
57.1-fold
Yadav et al. (2008)
CM-cellulose
Sephadex G-100 column
chromatography
Note: PG polygalacturonases (EC 3.2.1.15), PE pectinesterase (EC 3.1.1.11), PL pectin lyase
(EC4.2.2.10)
6 Fungal Pectinases: Production and Applications in Food Industries