NGS Technologies
4
Marius Eisele and Melanie Kappelmann-Fenzl
Contents
4.1 Introduction . . . .... ..... .... ..... .... ..... .... ..... ..... .... ..... .... ..... .... ..... .... ..... .... . 48
4.2 Illumina . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
4.3 Ion Torrent . . . ...... ...... ...... ...... ...... ...... ...... ...... ...... ...... ...... ....... ...... .... 51
4.4 Pacific Bioscience . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
4.5 Oxford Nanopore . .... .... ..... .... ..... .... .... ..... .... .... ..... .... .... ..... .... .... ..... .... 52
4.6 NGS Technologies: An Overview .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... . 54
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
What You Will Learn in This Chapter
There are a number of different companies that have developed and improved the
NGS technology immensely in the last 10 years. In this chapter an overview of the
most common technologies and their basic properties shall be given. Furthermore, it
will be shown which technologies can be used for specific scientific or clinical
questions and how they differ in their chemistry and output.
M. Eisele
Deggendorf Institute of Technology, Deggendorf, Germany
M. Kappelmann-Fenzl (*)
Deggendorf Institute of Technology, Deggendorf, Germany
Institute of Biochemistry (Emil-Fischer Center), Friedrich–Alexander University Erlangen–
Nürnberg, Erlangen, Germany
e-mail: melanie.kappelmann-fenzl@th-deg.de
# Springer Nature Switzerland AG 2021
M. Kappelmann-Fenzl (ed.), Next Generation Sequencing and Data Analysis, Learning
Materials in Biosciences, https://doi.org/10.1007/978-3-030-62490-3_4
47
4
Marius Eisele and Melanie Kappelmann-Fenzl
Contents
4.1 Introduction . . . .... ..... .... ..... .... ..... .... ..... ..... .... ..... .... ..... .... ..... .... ..... .... . 48
4.2 Illumina . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
4.3 Ion Torrent . . . ...... ...... ...... ...... ...... ...... ...... ...... ...... ...... ...... ....... ...... .... 51
4.4 Pacific Bioscience . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
4.5 Oxford Nanopore . .... .... ..... .... ..... .... .... ..... .... .... ..... .... .... ..... .... .... ..... .... 52
4.6 NGS Technologies: An Overview .... .... .... .... .... .... .... .... .... .... .... .... .... .... .... . 54
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
What You Will Learn in This Chapter
There are a number of different companies that have developed and improved the
NGS technology immensely in the last 10 years. In this chapter an overview of the
most common technologies and their basic properties shall be given. Furthermore, it
will be shown which technologies can be used for specific scientific or clinical
questions and how they differ in their chemistry and output.
M. Eisele
Deggendorf Institute of Technology, Deggendorf, Germany
M. Kappelmann-Fenzl (*)
Deggendorf Institute of Technology, Deggendorf, Germany
Institute of Biochemistry (Emil-Fischer Center), Friedrich–Alexander University Erlangen–
Nürnberg, Erlangen, Germany
e-mail: melanie.kappelmann-fenzl@th-deg.de
# Springer Nature Switzerland AG 2021
M. Kappelmann-Fenzl (ed.), Next Generation Sequencing and Data Analysis, Learning
Materials in Biosciences, https://doi.org/10.1007/978-3-030-62490-3_4
47