Library Construction for NGS
3
Melanie Kappelmann-Fenzl
Contents
3.1 Introduction . . . .... ..... .... ..... .... ..... .... ..... ..... .... ..... .... ..... .... ..... .... ..... .... . 40
3.2 Library Preparation Workflow . ... ... .... ... .... ... ... .... ... .... ... ... .... ... ... .... ... .... ... 40
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
What You Will Learn in This Chapter
After finishing this chapter, you will have a basic overview of the individual
experimental work steps regarding the NGS library preparation workflow, the connection between the scientific or clinical question and the choice of the
corresponding library preparation. For this purpose, the technical and molecular
biological relevance of the individual work steps is briefly described.
M. Kappelmann-Fenzl (*)
Deggendorf Institute of Technology, Deggendorf, Germany
Institute of Biochemistry (Emil-Fischer Center), Friedrich–Alexander University Erlangen–
Nürnberg, Erlangen, Germany
e-mail: melanie.kappelmann-fenzl@th-deg.de
# Springer Nature Switzerland AG 2021
M. Kappelmann-Fenzl (ed.), Next Generation Sequencing and Data Analysis, Learning
Materials in Biosciences, https://doi.org/10.1007/978-3-030-62490-3_3
39
3
Melanie Kappelmann-Fenzl
Contents
3.1 Introduction . . . .... ..... .... ..... .... ..... .... ..... ..... .... ..... .... ..... .... ..... .... ..... .... . 40
3.2 Library Preparation Workflow . ... ... .... ... .... ... ... .... ... .... ... ... .... ... ... .... ... .... ... 40
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
What You Will Learn in This Chapter
After finishing this chapter, you will have a basic overview of the individual
experimental work steps regarding the NGS library preparation workflow, the connection between the scientific or clinical question and the choice of the
corresponding library preparation. For this purpose, the technical and molecular
biological relevance of the individual work steps is briefly described.
M. Kappelmann-Fenzl (*)
Deggendorf Institute of Technology, Deggendorf, Germany
Institute of Biochemistry (Emil-Fischer Center), Friedrich–Alexander University Erlangen–
Nürnberg, Erlangen, Germany
e-mail: melanie.kappelmann-fenzl@th-deg.de
# Springer Nature Switzerland AG 2021
M. Kappelmann-Fenzl (ed.), Next Generation Sequencing and Data Analysis, Learning
Materials in Biosciences, https://doi.org/10.1007/978-3-030-62490-3_3
39