Description of parameters:
-q
phred score of 20
--minimum-length
the minimum sequence length (35)
-a
the adapter sequence for trimming
-o
the output file name
Fig. 11.2 A schematic illustration of a typical RNA-Seq experiment. Standard protocols for both
reference-based alignment/mapping and de novo assembly are shown
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-q
phred score of 20
--minimum-length
the minimum sequence length (35)
-a
the adapter sequence for trimming
-o
the output file name
Fig. 11.2 A schematic illustration of a typical RNA-Seq experiment. Standard protocols for both
reference-based alignment/mapping and de novo assembly are shown
11 Design and Analysis of RNA Sequencing Data
149
