40
Quality scores across all bases (Sanger / Illumina 1.9 encoding)
38
36
34
32
30
28
26
24
22
20
18
16
14
12
10
8
6
4
2
0
1 2 3 4 5 6 7 8 9 15–19 30–34 45–49 60–64 80–84 100–104
Position in read (bp)
120–124 140–144 165–169 185–189 205–209 225–229 245–249
Fig. 7.6 Bad per base quality graph. (source: https://rtsf.natsci.msu.edu/)
40
Quality scrores across all bases (Sanger / Illmina 1.9 encoding)
38
36
34
32
30
28
26
24
22
20
18
16
14
12
10
8
6
4
2
0 1 2 3 4 5 6 7 8 9 15–19 30–34
45–49 60–64
Position in read (bp)
75–79
90–94 105–109 120–124 135–139 150
Fig. 7.7 Good per base quality graph. (source: https://rtsf.natsci.msu.edu/)
7 NGS Data
95