References
1. Balvay L, Soto Rifo R, Ricci EP et al (2009)
Structural and functional diversity of viral
IRESes. Biochim Biophys Acta 1789
(9-10):542–557. https://doi.org/10.1016/j.
bbagrm.2009.07.005
2. Kieft JS (2008) Viral IRES RNA structures and
ribosome interactions. Trends Biochem Sci 33
(6):274–283.
https://doi.org/10.1016/j.
tibs.2008.04.007
3. Thompson SR (2012) Tricks an IRES uses to
enslave ribosomes. Trends Microbiol 20
(11):558–566. https://doi.org/10.1016/j.
tim.2012.08.002
4. Filbin ME, Kieft JS (2009) Toward a structural
understanding of IRES RNA function. Curr
Opin Struct Biol 19(3):267–276. https://doi.
org/10.1016/j.sbi.2009.03.005
5. Johnson AG, Grosely R, Petrov AN, Puglisi JD
(2017) Dynamics of IRES-mediated translation. Philos Trans R Soc Lond Ser B Biol Sci
372(1716). https://doi.org/10.1098/rstb.
2016.0177rstb.2016.0177
6. Bugaud O, Barbier N, Chommy H et al (2017)
Kinetics of CrPV and HCV IRES-mediated
eukaryotic translation using single-molecule
fluorescence
microscopy.
RNA
23
(11):1626–1635. https://doi.org/10.1261/
rna.061523.117rna.061523
7. Petrov A, Grosely R, Chen J et al (2016) Multiple parallel pathways of translation initiation
on the CrPV IRES. Mol Cell 62(1):92–103.
https://doi.org/10.1016/j.molcel.2016.03.
020
8. Muhs M, Hilal T, Mielke T et al (2015) CryoEM of ribosomal 80S complexes with termination factors reveals the translocated cricket
paralysis virus IRES. Mol Cell 57
(3):422–432.
https://doi.org/10.1016/j.
molcel.2014.12.016
9. Murray J, Savva CG, Shin BS et al (2016)
Structural characterization of ribosome recruitment and translocation by type IV IRES. Elife
5:e13567.
https://doi.org/10.7554/eLife.
13567
10. Kerr CH, Ma ZW, Jang CJ et al (2016) Molecular analysis of the factorless internal ribosome
entry site in cricket paralysis virus infection. Sci
Rep 6:37319. https://doi.org/10.1038/
srep37319
11. Pestova TV, Lomakin IB, Hellen CU (2004)
Position of the CrPV IRES on the 40S subunit
and factor dependence of IRES/80S ribosome
assembly. EMBO Rep 5(9):906–913. https://
doi.org/10.1038/sj.embor.7400240
12. Knezevic J, Langer A, Hampel PA et al (2012)
Quantitation of affinity, avidity, and binding
kinetics of protein analytes with a dynamically
switchable biosurface. J Am Chem Soc 134
(37):15225–15228.
https://doi.org/10.
1021/ja3061276
13. Langer A, Hampel PA, Kaiser W et al (2013)
Protein analysis by time-resolved measurements with an electro-switchable DNA chip.
Nat Commun 4:2099. https://doi.org/10.
1038/ncomms3099
14. Rant U, Pringsheim E, Kaiser W et al (2009)
Detection and size analysis of proteins with
switchable DNA layers. Nano Lett 9
(4):1290–1295. https://doi.org/10.1021/
nl8026789
15. Kroener F, Heerwig A, Kaiser W et al (2017)
Electrical actuation of a DNA origami Nanolever on an electrode. J Am Chem Soc 139
(46):16510–16513.
https://doi.org/10.
1021/jacs.7b10862
16. Clery A, Sohier TJM, Welte T et al (2017)
switchSENSE: A new technology to study
protein-RNA
interactions.
Methods
118-119:137–145.
https://doi.org/10.
1016/j.ymeth.2017.03.004
17. Ben-Shem A, Garreau de Loubresse N, Melnikov S et al (2011) The structure of the eukaryotic ribosome at 3.0 a resolution. Science 334
(6062):1524–1529.
https://doi.org/10.
1126/science.1212642
350
Emma Schenckbecher et al.
1. Balvay L, Soto Rifo R, Ricci EP et al (2009)
Structural and functional diversity of viral
IRESes. Biochim Biophys Acta 1789
(9-10):542–557. https://doi.org/10.1016/j.
bbagrm.2009.07.005
2. Kieft JS (2008) Viral IRES RNA structures and
ribosome interactions. Trends Biochem Sci 33
(6):274–283.
https://doi.org/10.1016/j.
tibs.2008.04.007
3. Thompson SR (2012) Tricks an IRES uses to
enslave ribosomes. Trends Microbiol 20
(11):558–566. https://doi.org/10.1016/j.
tim.2012.08.002
4. Filbin ME, Kieft JS (2009) Toward a structural
understanding of IRES RNA function. Curr
Opin Struct Biol 19(3):267–276. https://doi.
org/10.1016/j.sbi.2009.03.005
5. Johnson AG, Grosely R, Petrov AN, Puglisi JD
(2017) Dynamics of IRES-mediated translation. Philos Trans R Soc Lond Ser B Biol Sci
372(1716). https://doi.org/10.1098/rstb.
2016.0177rstb.2016.0177
6. Bugaud O, Barbier N, Chommy H et al (2017)
Kinetics of CrPV and HCV IRES-mediated
eukaryotic translation using single-molecule
fluorescence
microscopy.
RNA
23
(11):1626–1635. https://doi.org/10.1261/
rna.061523.117rna.061523
7. Petrov A, Grosely R, Chen J et al (2016) Multiple parallel pathways of translation initiation
on the CrPV IRES. Mol Cell 62(1):92–103.
https://doi.org/10.1016/j.molcel.2016.03.
020
8. Muhs M, Hilal T, Mielke T et al (2015) CryoEM of ribosomal 80S complexes with termination factors reveals the translocated cricket
paralysis virus IRES. Mol Cell 57
(3):422–432.
https://doi.org/10.1016/j.
molcel.2014.12.016
9. Murray J, Savva CG, Shin BS et al (2016)
Structural characterization of ribosome recruitment and translocation by type IV IRES. Elife
5:e13567.
https://doi.org/10.7554/eLife.
13567
10. Kerr CH, Ma ZW, Jang CJ et al (2016) Molecular analysis of the factorless internal ribosome
entry site in cricket paralysis virus infection. Sci
Rep 6:37319. https://doi.org/10.1038/
srep37319
11. Pestova TV, Lomakin IB, Hellen CU (2004)
Position of the CrPV IRES on the 40S subunit
and factor dependence of IRES/80S ribosome
assembly. EMBO Rep 5(9):906–913. https://
doi.org/10.1038/sj.embor.7400240
12. Knezevic J, Langer A, Hampel PA et al (2012)
Quantitation of affinity, avidity, and binding
kinetics of protein analytes with a dynamically
switchable biosurface. J Am Chem Soc 134
(37):15225–15228.
https://doi.org/10.
1021/ja3061276
13. Langer A, Hampel PA, Kaiser W et al (2013)
Protein analysis by time-resolved measurements with an electro-switchable DNA chip.
Nat Commun 4:2099. https://doi.org/10.
1038/ncomms3099
14. Rant U, Pringsheim E, Kaiser W et al (2009)
Detection and size analysis of proteins with
switchable DNA layers. Nano Lett 9
(4):1290–1295. https://doi.org/10.1021/
nl8026789
15. Kroener F, Heerwig A, Kaiser W et al (2017)
Electrical actuation of a DNA origami Nanolever on an electrode. J Am Chem Soc 139
(46):16510–16513.
https://doi.org/10.
1021/jacs.7b10862
16. Clery A, Sohier TJM, Welte T et al (2017)
switchSENSE: A new technology to study
protein-RNA
interactions.
Methods
118-119:137–145.
https://doi.org/10.
1016/j.ymeth.2017.03.004
17. Ben-Shem A, Garreau de Loubresse N, Melnikov S et al (2011) The structure of the eukaryotic ribosome at 3.0 a resolution. Science 334
(6062):1524–1529.
https://doi.org/10.
1126/science.1212642
350
Emma Schenckbecher et al.