Acknowledgments
Expert advice provided by Dr. Martin Webb (The Francis Crick
Institute) in the preparation of this chapter is gratefully acknowledged. I would also like to thank Dr. Elizabeth Morris (The Francis
Crick Institute) and Dr. Martin Webb for comments on the
manuscript.
This work was supported by the Francis Crick Institute which
receives its core funding from Cancer Research UK (FC001999),
the UK Medical Research Council (FC001999), and the Wellcome
Trust (FC001999).
References
1. Brune M, Hunter JL, Corrie JE, Webb MR
(1994) Direct, real-time measurement of
rapid inorganic phosphate release using a
novel fluorescent probe and its application to
actomyosin subfragment 1 ATPase. Biochemistry 33(27):8262–8271
2. He ZH, Chillingworth RK, Brune M, Corrie
JE, Trentham DR, Webb MR, Ferenczi MA
(1997) ATPase kinetics on activation of rabbit
and frog permeabilized isometric muscle fibres:
a real time phosphate assay. J Physiol 501
(1):125–148
3. He ZH, Chillingworth RK, Brune M, Corrie
JET, Webb MR, Ferenczi MA (1999) The efficiency of contraction in rabbit skeletal muscle
fibres, determined from the rate of release of
inorganic
phosphate.
J
Physiol
517
(3):839–854
4. Hirshberg M, Henrick K, Haire LL, Vasisht N,
Brune M, Corrie JE, Webb MR (1998) Crystal
structure of phosphate binding protein labeled
with a coumarin fluorophore, a probe for inorganic
phosphate.
Biochemistry
37
(29):10381–10385
5. Brune M, Hunter JL, Howell SA, Martin SR,
Hazlett TL, Corrie JE, Webb MR (1998)
Mechanism of inorganic phosphate interaction
with phosphate binding protein from Escherichia coli. Biochemistry 37(29):10370–10380
6. Okoh MP, Hunter JL, Corrie JE, Webb MR
(2006) A biosensor for inorganic phosphate
using a rhodamine-labeled phosphate binding
protein. Biochemistry 45(49):14764–14771
7. Fo ¨rster T, Ko ¨nig E (1957) Absorptionsspektren und Fluoreszenzeigenschaften konzentrierter Lo ¨sungen organischer Farbstoffe. Z
Elektrochem 61:344–348
8. Selwyn JE, Steinfeld JI (1972) Aggregation
equilibria of xanthene dyes. J Phys Chem
76:762–774
9. Solscheid C, Kunzelmann S, Davis CT, Hunter
JL, Nofer A, Webb MR (2015) Development
of a Reagentless biosensor for inorganic phosphate, applicable over a wide concentration
range. Biochemistry 54(32):5054–5062
10. Gu H, Lalonde S, Okumoto S, Looger LL,
Scharff-Poulsen
AM,
Grossman
AR,
Kossmann J, Jakobsen I, Frommer WB
(2006) A novel analytical method for in vivo
phosphate tracking. FEBS Lett 580
(25):5885–5893
11. Kunzelmann S, Webb MR (2009) A biosensor
for fluorescent determination of ADP with
high time resolution. J Biol Chem 284
(48):33130–33138
12. Kunzelmann S, Webb MR (2010) A fluorescent, reagentless biosensor for ADP based on
tetramethylrhodamine-labeled ParM. ACS
Chem Biol 5(4):415–425
13. Kunzelmann S, Webb MR (2011) Fluorescence detection of GDP in real time with the
reagentless biosensor rhodamine-ParM. Biochem J 440(1):43–49
14. Vancraenenbroeck R, Webb MR (2015) A fluorescent, Reagentless biosensor for ATP, based
on Malonyl-coenzyme a Synthetase. ACS
Chem Biol 10(11):2650–2657
15. Berg J, Hung YP, Yellen G (2009) A genetically
encoded fluorescent reporter of ATP:ADP
ratio. Nat Methods 6(2):161–166
16. Brune M, Corrie JE, Webb MR (2001) A fluorescent sensor of the phosphorylation state of
nucleoside diphosphate kinase and its use to
monitor nucleoside diphosphate concentrations in real time. Biochemistry 40
(16):5087–5094
17. de Lorimier RM, Smith JJ, Dwyer MA, Looger
LL, Sali KM, Paavola CD, Rizk SS, Sadigov S,
Conrad DW, Loew L et al (2002) Construction
316
Simone Kunzelmann
Expert advice provided by Dr. Martin Webb (The Francis Crick
Institute) in the preparation of this chapter is gratefully acknowledged. I would also like to thank Dr. Elizabeth Morris (The Francis
Crick Institute) and Dr. Martin Webb for comments on the
manuscript.
This work was supported by the Francis Crick Institute which
receives its core funding from Cancer Research UK (FC001999),
the UK Medical Research Council (FC001999), and the Wellcome
Trust (FC001999).
References
1. Brune M, Hunter JL, Corrie JE, Webb MR
(1994) Direct, real-time measurement of
rapid inorganic phosphate release using a
novel fluorescent probe and its application to
actomyosin subfragment 1 ATPase. Biochemistry 33(27):8262–8271
2. He ZH, Chillingworth RK, Brune M, Corrie
JE, Trentham DR, Webb MR, Ferenczi MA
(1997) ATPase kinetics on activation of rabbit
and frog permeabilized isometric muscle fibres:
a real time phosphate assay. J Physiol 501
(1):125–148
3. He ZH, Chillingworth RK, Brune M, Corrie
JET, Webb MR, Ferenczi MA (1999) The efficiency of contraction in rabbit skeletal muscle
fibres, determined from the rate of release of
inorganic
phosphate.
J
Physiol
517
(3):839–854
4. Hirshberg M, Henrick K, Haire LL, Vasisht N,
Brune M, Corrie JE, Webb MR (1998) Crystal
structure of phosphate binding protein labeled
with a coumarin fluorophore, a probe for inorganic
phosphate.
Biochemistry
37
(29):10381–10385
5. Brune M, Hunter JL, Howell SA, Martin SR,
Hazlett TL, Corrie JE, Webb MR (1998)
Mechanism of inorganic phosphate interaction
with phosphate binding protein from Escherichia coli. Biochemistry 37(29):10370–10380
6. Okoh MP, Hunter JL, Corrie JE, Webb MR
(2006) A biosensor for inorganic phosphate
using a rhodamine-labeled phosphate binding
protein. Biochemistry 45(49):14764–14771
7. Fo ¨rster T, Ko ¨nig E (1957) Absorptionsspektren und Fluoreszenzeigenschaften konzentrierter Lo ¨sungen organischer Farbstoffe. Z
Elektrochem 61:344–348
8. Selwyn JE, Steinfeld JI (1972) Aggregation
equilibria of xanthene dyes. J Phys Chem
76:762–774
9. Solscheid C, Kunzelmann S, Davis CT, Hunter
JL, Nofer A, Webb MR (2015) Development
of a Reagentless biosensor for inorganic phosphate, applicable over a wide concentration
range. Biochemistry 54(32):5054–5062
10. Gu H, Lalonde S, Okumoto S, Looger LL,
Scharff-Poulsen
AM,
Grossman
AR,
Kossmann J, Jakobsen I, Frommer WB
(2006) A novel analytical method for in vivo
phosphate tracking. FEBS Lett 580
(25):5885–5893
11. Kunzelmann S, Webb MR (2009) A biosensor
for fluorescent determination of ADP with
high time resolution. J Biol Chem 284
(48):33130–33138
12. Kunzelmann S, Webb MR (2010) A fluorescent, reagentless biosensor for ADP based on
tetramethylrhodamine-labeled ParM. ACS
Chem Biol 5(4):415–425
13. Kunzelmann S, Webb MR (2011) Fluorescence detection of GDP in real time with the
reagentless biosensor rhodamine-ParM. Biochem J 440(1):43–49
14. Vancraenenbroeck R, Webb MR (2015) A fluorescent, Reagentless biosensor for ATP, based
on Malonyl-coenzyme a Synthetase. ACS
Chem Biol 10(11):2650–2657
15. Berg J, Hung YP, Yellen G (2009) A genetically
encoded fluorescent reporter of ATP:ADP
ratio. Nat Methods 6(2):161–166
16. Brune M, Corrie JE, Webb MR (2001) A fluorescent sensor of the phosphorylation state of
nucleoside diphosphate kinase and its use to
monitor nucleoside diphosphate concentrations in real time. Biochemistry 40
(16):5087–5094
17. de Lorimier RM, Smith JJ, Dwyer MA, Looger
LL, Sali KM, Paavola CD, Rizk SS, Sadigov S,
Conrad DW, Loew L et al (2002) Construction
316
Simone Kunzelmann