214
P. T. Lacava et al.
bacteria belonged to the Betaproteobacteria, Gammaproteobacteria, and Firmicutes
phyla. Phylogenetic analyses of cloned sequences from Firmicutes revealed that most
sequences fell into 3 major genera: Bacillus, Staphylococcus, and Paenibacillus. In
this case, we found similar results to those reported by Oliveira et al. (2013) regarding
the diversity of endophytic bacteria isolated from C. arabica L. cherries.
Silva et al. (2012) reported the potential of endophytic bacteria isolated from
healthy Coffea arabica L. and Coffea robusta L. from Pedreira, Mococa, and
Pindorama counties, State of São Paulo, Brazil. Previously, promising growthpromoter endophytic strains were evaluated qualitatively in vitro for the production
of phosphatase, indole acetic acid (IAA), siderophores, cytokinins, and gibberellins
(CYT/GIB). A total of two hundred seventeen strains were evaluated for their potential to promote the growth of coffee seedlings in vivo. According to the authors
(Silva et al. 2012), the growth-promoting indexes of 119 bacterial strains were
higher than those of the control. Additionally, the Scott–Knott cluster test indicated that the indexes of six bacterial strains, 85G (Escherichia fergusonii), 161G,
163G, 160G, 150G (Acinetobacter calcoaceticus), and 109G (Salmonella enterica),
differed significantly from that of the control. However, of these six strains showed
with the best performance in vivo, only two (161G and 160G) produced phosphatase
as a positive plant-growth promotion parameter when tested in vitro.
In our research, we have investigated the ability of endophytes from different host
plants to promote plant growth, including phosphate solubilization. In this context,
Andrade (2019) performed a study that included the isolation, biochemical characterization, identification, and analysis of the genetic diversity of the endophytic
bacterial community associated with C. arabica L. from conventional and organic
cultivation sources. The results demonstrated that 342 endophytic bacterial isolates
were evaluated for in vitro growth factors, where, about 64.33% isolates were solubilized inorganic phosphate. The identification of endophytic strains was performed by
bacterial cell protein analysis by matrix-assisted laser desorption/ionization-time-offlight (MALDI-TOF) and by partial sequencing of the 16S rDNA gene. The genera
identified were Arthrobacter, Bacillus, Cronobacter, Enterobacter, Erwinia, Klebsiella, Kosakonia, Kurthia, Lysinibacillus, Microbacterium, Pantoea, Pseudomonas,
and Rhizobium.
In this case study (Andrade 2019), the endophytic strains with the best performance for in vitro growth factors, including phosphate solubilization, were selected
for inoculation in coffee seedlings. Ten endophytic strains were selected based on the
biochemical characterization previously performed in vitro about the potential for
plant growth promotion. The treatments consisted of a control (C) with 8 replicates
where the seedlings were inoculated only with PBS buffer and 10 treatments (T1 to
T10) with 8 replicates which consisted of weekly inoculation of the seedlings with
a bacterial suspension of CRM 162 - Erwinia bilingiae (T1); CRM 202 - Kosakonia
cowanii (T2); CRA 241 Enterobacter tabaci (T3); CRA 250 Enterobacter tabaci
(T4); CRA 298 - Pantoea brenneri (T5) from conventionally grown coffee; OFR
175 - Lysinibacillus mangiferihumi (T6); OFR 176-Lysinibacillus mangiferihumi
(T7); OFR 164 - Klebsiella pneumoniae (T8); OFO 340 - Enterobacter bugandensis
(T9); or ORM 326 - Klebsiella michiganensis (T10). A total of 180 days after the first
P. T. Lacava et al.
bacteria belonged to the Betaproteobacteria, Gammaproteobacteria, and Firmicutes
phyla. Phylogenetic analyses of cloned sequences from Firmicutes revealed that most
sequences fell into 3 major genera: Bacillus, Staphylococcus, and Paenibacillus. In
this case, we found similar results to those reported by Oliveira et al. (2013) regarding
the diversity of endophytic bacteria isolated from C. arabica L. cherries.
Silva et al. (2012) reported the potential of endophytic bacteria isolated from
healthy Coffea arabica L. and Coffea robusta L. from Pedreira, Mococa, and
Pindorama counties, State of São Paulo, Brazil. Previously, promising growthpromoter endophytic strains were evaluated qualitatively in vitro for the production
of phosphatase, indole acetic acid (IAA), siderophores, cytokinins, and gibberellins
(CYT/GIB). A total of two hundred seventeen strains were evaluated for their potential to promote the growth of coffee seedlings in vivo. According to the authors
(Silva et al. 2012), the growth-promoting indexes of 119 bacterial strains were
higher than those of the control. Additionally, the Scott–Knott cluster test indicated that the indexes of six bacterial strains, 85G (Escherichia fergusonii), 161G,
163G, 160G, 150G (Acinetobacter calcoaceticus), and 109G (Salmonella enterica),
differed significantly from that of the control. However, of these six strains showed
with the best performance in vivo, only two (161G and 160G) produced phosphatase
as a positive plant-growth promotion parameter when tested in vitro.
In our research, we have investigated the ability of endophytes from different host
plants to promote plant growth, including phosphate solubilization. In this context,
Andrade (2019) performed a study that included the isolation, biochemical characterization, identification, and analysis of the genetic diversity of the endophytic
bacterial community associated with C. arabica L. from conventional and organic
cultivation sources. The results demonstrated that 342 endophytic bacterial isolates
were evaluated for in vitro growth factors, where, about 64.33% isolates were solubilized inorganic phosphate. The identification of endophytic strains was performed by
bacterial cell protein analysis by matrix-assisted laser desorption/ionization-time-offlight (MALDI-TOF) and by partial sequencing of the 16S rDNA gene. The genera
identified were Arthrobacter, Bacillus, Cronobacter, Enterobacter, Erwinia, Klebsiella, Kosakonia, Kurthia, Lysinibacillus, Microbacterium, Pantoea, Pseudomonas,
and Rhizobium.
In this case study (Andrade 2019), the endophytic strains with the best performance for in vitro growth factors, including phosphate solubilization, were selected
for inoculation in coffee seedlings. Ten endophytic strains were selected based on the
biochemical characterization previously performed in vitro about the potential for
plant growth promotion. The treatments consisted of a control (C) with 8 replicates
where the seedlings were inoculated only with PBS buffer and 10 treatments (T1 to
T10) with 8 replicates which consisted of weekly inoculation of the seedlings with
a bacterial suspension of CRM 162 - Erwinia bilingiae (T1); CRM 202 - Kosakonia
cowanii (T2); CRA 241 Enterobacter tabaci (T3); CRA 250 Enterobacter tabaci
(T4); CRA 298 - Pantoea brenneri (T5) from conventionally grown coffee; OFR
175 - Lysinibacillus mangiferihumi (T6); OFR 176-Lysinibacillus mangiferihumi
(T7); OFR 164 - Klebsiella pneumoniae (T8); OFO 340 - Enterobacter bugandensis
(T9); or ORM 326 - Klebsiella michiganensis (T10). A total of 180 days after the first