8 Biotechnology and Bioinformatics of Endophytes …
185
Fig. 8.2 Pie-chart of endophytic bacteria community based on 16S-rDNA
can be revealed through the application of diversified nutrient media (Christina et al.
2013; Goulart et al. 2019; Srivastava et al. 2019). These approaches have several
advantages and drawbacks that are discussed in the two following sections.
a. Culture dependent approach
Cultivable endophytes could be easily isolated, manipulated, and further exploited
in the development of different fields (agricultural, medical, industrial, etc.) as
reviewed by Maela and Serepa-Dlamini (2019). The inherent limitation of this
approach is that it is estimated to detect 0.1–1% of the existing microbiome (Stewart
2012; Belbahri et al. 2017; Mefteh et al. 2017, 2019).
185
Fig. 8.2 Pie-chart of endophytic bacteria community based on 16S-rDNA
can be revealed through the application of diversified nutrient media (Christina et al.
2013; Goulart et al. 2019; Srivastava et al. 2019). These approaches have several
advantages and drawbacks that are discussed in the two following sections.
a. Culture dependent approach
Cultivable endophytes could be easily isolated, manipulated, and further exploited
in the development of different fields (agricultural, medical, industrial, etc.) as
reviewed by Maela and Serepa-Dlamini (2019). The inherent limitation of this
approach is that it is estimated to detect 0.1–1% of the existing microbiome (Stewart
2012; Belbahri et al. 2017; Mefteh et al. 2017, 2019).