1. BIAcore Running buffer: 25 mM HEPES pH 7.4 and 150 mM
NaCl. Weigh 5.957 g of HEPES and 8.766 g of NaCl and
transfer to a glass beaker. Add about 900 mL of deionized
water to the glass beaker. Mix and adjust the pH with NaOH
to 7.4. Transfer to a 1 L graduated cylinder and adjust to 1 L
Fig. 2 Representation of a sensorgram: a real-time plot of binding response over time. Upon injection of the
analyte (sample) (b), the signal increases till equilibrium (c) is reached, allowing observation of the association
rate. When the analyte is no longer provided (d), dissociation can be observed. After regeneration (e), another
measurement can take place
Fig. 1 Schematic representation of SA sensor chip capturing biotinylated ligands.
Streptavidin is covalently attached to dextran matrix for the nonreversible
capture of biotinylated molecules
PDZ-Peptide and PDZ-Lipid Interactions by SPR
77
NaCl. Weigh 5.957 g of HEPES and 8.766 g of NaCl and
transfer to a glass beaker. Add about 900 mL of deionized
water to the glass beaker. Mix and adjust the pH with NaOH
to 7.4. Transfer to a 1 L graduated cylinder and adjust to 1 L
Fig. 2 Representation of a sensorgram: a real-time plot of binding response over time. Upon injection of the
analyte (sample) (b), the signal increases till equilibrium (c) is reached, allowing observation of the association
rate. When the analyte is no longer provided (d), dissociation can be observed. After regeneration (e), another
measurement can take place
Fig. 1 Schematic representation of SA sensor chip capturing biotinylated ligands.
Streptavidin is covalently attached to dextran matrix for the nonreversible
capture of biotinylated molecules
PDZ-Peptide and PDZ-Lipid Interactions by SPR
77