Caliper data
Crude extract
in the
sample?
yes
no
Change
parameters
Baseline
correction
Normalization
Horizontal
translation and
dilation
If error
If error
Secondary
correction
Superimposition
Check
the data
BI calculation
If error
Fig. 2 Flowchart of the computational strategy presented in this protocol. Circle:
input/output; rectangle with lines: step for processing data; diamond: decision;
inverted trapezoid: manual process done by the user. The “If error” statement
stands for the quality check of the process. When the step output does not match
the quality criteria (See “Checking the data quality” paragraph), it is recommended to return to the baseline correction step in order to change one or
several parameters and to restart the algorithm
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Pau Jane ´ et al.