26. Caria S, Stewart BZ, Jin R, Smith BJ, Humbert
PO, Kvansakul M (2019) Structural analysis of
phosphorylation-associated interactions of
human MCC with scribble PDZ domains.
FEBS J 286:4910–4925
27. Feng W, Zhang M (2009) Organization and
dynamics of PDZ-domain-related supramodules in the postsynaptic density. Nat Rev Neurosci 10:87–99
28. Ren J, Feng L, Bai Y, Pei H, Yuan Z, Feng W
(2015) Interdomain interface-mediated target
recognition by the scribble PDZ34 supramodule. Biochem J 468:133–144
29. Caria S, Magtoto CM, Samiei T, Portela M,
Lim KYB, How JY, Stewart BZ, Humbert
PO, Richardson HE, Kvansakul M (2018)
Drosophila melanogaster Guk-holder interacts
with the scribbled PDZ1 domain and regulates
epithelial development with scribbled and discs
large. J Biol Chem 293(12):4519–4531
30. How JY, Caria S, Humbert PO, Kvansakul M
(2019) Crystal structure of the human scribble
PDZ1 domain bound to the PDZ-binding
motif of APC. FEBS Lett 593:533–542
31. Zhang Z, Li H, Chen L, Lu X, Zhang J, Xu P,
Lin K, Wu G (2011) Molecular basis for the
recognition of adenomatous polyposis coli by
the discs large 1 protein. PLoS One 6:e23507
32. Slep KC (2012) Structure of the human discs
large 1 PDZ2- adenomatous polyposis coli
cytoskeletal polarity complex: insight into peptide engagement and PDZ clustering. PLoS
One 7:e50097
33. Mischo A, Ohlenschl€ ager O, Hortschansky P,
Ramachandran R, Go ¨rlach M (2013)
Structural insights into a wildtype domain of
the oncoprotein E6 and its interaction with a
PDZ domain. PLoS One 8:e62584
34. Liu Y, Henry GD, Hegde RS, Baleja JD (2007)
Solution structure of the hDlg/SAP97 PDZ2
domain and its mechanism of interaction with
HPV-18 papillomavirus E6 protein. Biochemistry 46:10864–10874
35. Zhang Y, Dasgupta J, Ma RZ, Banks L,
Thomas M, Chen XS (2007) Structures of a
human papillomavirus (HPV) E6 polypeptide
bound to MAGUK proteins: mechanisms of
targeting tumor suppressors by a high-risk
HPV oncoprotein. J Virol 81:3618–3626
36. Sainlos M, Iskenderian-Epps WS, Olivier NB,
Choquet D, Imperiali B (2013) Caged monoand divalent ligands for light-assisted disruption of PDZ domain-mediated interactions. J
Am Chem Soc 135:4580–4583
37. Wang W, Weng J, Zhang X, Liu M, Zhang M
(2009) Creating conformational entropy by
increasing interdomain mobility in ligand binding regulation: a revisit to N-terminal tandem
PDZ domains of PSD-95. J Am Chem Soc
131:787–796
38. Zeng M, Shang Y, Araki Y, Guo T, Huganir
RL, Zhang M (2016) Phase transition in postsynaptic densities underlies formation of synaptic complexes and synaptic plasticity. Cell
166:1163–1175
39. Raman AS, White KI, Ranganathan R (2016)
Origins of allostery and evolvability in proteins:
a case study. Cell 166:468–480
Biophysics of Scribble Module PDZ Interactions
135
PO, Kvansakul M (2019) Structural analysis of
phosphorylation-associated interactions of
human MCC with scribble PDZ domains.
FEBS J 286:4910–4925
27. Feng W, Zhang M (2009) Organization and
dynamics of PDZ-domain-related supramodules in the postsynaptic density. Nat Rev Neurosci 10:87–99
28. Ren J, Feng L, Bai Y, Pei H, Yuan Z, Feng W
(2015) Interdomain interface-mediated target
recognition by the scribble PDZ34 supramodule. Biochem J 468:133–144
29. Caria S, Magtoto CM, Samiei T, Portela M,
Lim KYB, How JY, Stewart BZ, Humbert
PO, Richardson HE, Kvansakul M (2018)
Drosophila melanogaster Guk-holder interacts
with the scribbled PDZ1 domain and regulates
epithelial development with scribbled and discs
large. J Biol Chem 293(12):4519–4531
30. How JY, Caria S, Humbert PO, Kvansakul M
(2019) Crystal structure of the human scribble
PDZ1 domain bound to the PDZ-binding
motif of APC. FEBS Lett 593:533–542
31. Zhang Z, Li H, Chen L, Lu X, Zhang J, Xu P,
Lin K, Wu G (2011) Molecular basis for the
recognition of adenomatous polyposis coli by
the discs large 1 protein. PLoS One 6:e23507
32. Slep KC (2012) Structure of the human discs
large 1 PDZ2- adenomatous polyposis coli
cytoskeletal polarity complex: insight into peptide engagement and PDZ clustering. PLoS
One 7:e50097
33. Mischo A, Ohlenschl€ ager O, Hortschansky P,
Ramachandran R, Go ¨rlach M (2013)
Structural insights into a wildtype domain of
the oncoprotein E6 and its interaction with a
PDZ domain. PLoS One 8:e62584
34. Liu Y, Henry GD, Hegde RS, Baleja JD (2007)
Solution structure of the hDlg/SAP97 PDZ2
domain and its mechanism of interaction with
HPV-18 papillomavirus E6 protein. Biochemistry 46:10864–10874
35. Zhang Y, Dasgupta J, Ma RZ, Banks L,
Thomas M, Chen XS (2007) Structures of a
human papillomavirus (HPV) E6 polypeptide
bound to MAGUK proteins: mechanisms of
targeting tumor suppressors by a high-risk
HPV oncoprotein. J Virol 81:3618–3626
36. Sainlos M, Iskenderian-Epps WS, Olivier NB,
Choquet D, Imperiali B (2013) Caged monoand divalent ligands for light-assisted disruption of PDZ domain-mediated interactions. J
Am Chem Soc 135:4580–4583
37. Wang W, Weng J, Zhang X, Liu M, Zhang M
(2009) Creating conformational entropy by
increasing interdomain mobility in ligand binding regulation: a revisit to N-terminal tandem
PDZ domains of PSD-95. J Am Chem Soc
131:787–796
38. Zeng M, Shang Y, Araki Y, Guo T, Huganir
RL, Zhang M (2016) Phase transition in postsynaptic densities underlies formation of synaptic complexes and synaptic plasticity. Cell
166:1163–1175
39. Raman AS, White KI, Ranganathan R (2016)
Origins of allostery and evolvability in proteins:
a case study. Cell 166:468–480
Biophysics of Scribble Module PDZ Interactions
135