(6):3608–3616. https://doi.org/10.1128/
AAC.02896-15
11. Gold B, Roberts J, Ling Y, Quezada LL,
Glasheen J, Ballinger E, Somersan-Karakaya S,
Warrier T, Warren JD, Nathan C (2015) Rapid,
semiquantitative assay to discriminate among
compounds with activity against replicating or
nonreplicating Mycobacterium tuberculosis.
Antimicrob
Agents
Chemother
59
(10):6521–6538. https://doi.org/10.1128/
AAC.00803-15
12. Ballell L, Bates RH, Young RJ, AlvarezGomez D, Alvarez-Ruiz E, Barroso V,
Blanco D, Crespo B, Escribano J,
Gonzalez R, Lozano S, Huss S, SantosVillarejo A, Martin-Plaza JJ, Mendoza A,
Rebollo-Lopez MJ, Remuinan-Blanco M,
Lavandera JL, Perez-Herran E, Gamo-Benito
FJ, Garcia-Bustos JF, Barros D, Castro JP,
Cammack N (2013) Fueling open-source
drug discovery: 177 small-molecule leads
against tuberculosis. Chem Med Chem 8
(2):313–321.
https://doi.org/10.1002/
cmdc.201200428
13. Pethe K, Sequeira PC, Agarwalla S, Rhee K,
Kuhen K, Phong WY, Patel V, Beer D, Walker
JR, Duraiswamy J, Jiricek J, Keller TH,
Chatterjee A, Tan MP, Ujjini M, Rao SP,
Camacho L, Bifani P, Mak PA, Ma I, Barnes
SW, Chen Z, Plouffe D, Thayalan P, Ng SH,
Au M, Lee BH, Tan BH, Ravindran S,
Nanjundappa M, Lin X, Goh A, Lakshminarayana SB, Shoen C, Cynamon M, Kreiswirth B,
Dartois V, Peters EC, Glynne R, Brenner S,
Dick T (2010) A chemical genetic screen in
Mycobacterium tuberculosis identifies carbonsource-dependent growth inhibitors devoid of
in vivo efficacy. Nat Commun 1:57. https://
doi.org/10.1038/ncomms1060
14. Manjunatha UH, Smith PW (2015) Perspective: challenges and opportunities in TB drug
discovery from phenotypic screening. Bioorg
Med Chem 23(16):5087–5097. https://doi.
org/10.1016/j.bmc.2014.12.031
15. Ioerger TR, O’Malley T, Liao R, Guinn KM,
Hickey MJ, Mohaideen N, Murphy KC, Boshoff HI, Mizrahi V, Rubin EJ, Sassetti CM,
Barry CE 3rd, Sherman DR, Parish T, Sacchettini JC (2013) Identification of new drug targets
and
resistance
mechanisms
in
Mycobacterium tuberculosis. PLoS One 8(9):
e75245. https://doi.org/10.1371/journal.
pone.0075245
16. Stuehr DJ, Marletta MA (1985) Mammalian
nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide. Proc Natl Acad
Sci U S A 82(22):7738–7742. https://doi.
org/10.1073/pnas.82.22.7738
17. Blanco-Ruano D, Roberts DM, Gonzalez-DelRio R, Alvarez D, Rebollo MJ, Perez-Herran E,
Mendoza A (2015) Antimicrobial susceptibility
testing for Mycobacterium sp. Methods Mol
Biol 1285:257–268. https://doi.org/10.
1007/978-1-4939-2450-9_15
18. Cho S, Lee HS, Franzblau S (2015) Microplate
alamar blue assay (MABA) and low oxygen
recovery assay (LORA) for Mycobacterium
tuberculosis.
Methods
Mol
Biol
1285:281–292.
https://doi.org/10.1007/
978-1-4939-2450-9_17
19. Domenech P, Reed MB (2009) Rapid and
spontaneous loss of phthiocerol dimycocerosate (PDIM) from Mycobacterium tuberculosis
grown in vitro: implications for virulence studies. Microbiology 155(Pt 11):3532–3543.
https://doi.org/10.1099/mic.0.029199-0
20. Slayden RA, Barry CE 3rd (2001) Analysis of
the lipids of Mycobacterium tuberculosis.
Methods Mol Med 54:229–245. https://doi.
org/10.1385/1-59259-147-7:229
MIC Determination for Mycobacterium Tuberculosis
609
AAC.02896-15
11. Gold B, Roberts J, Ling Y, Quezada LL,
Glasheen J, Ballinger E, Somersan-Karakaya S,
Warrier T, Warren JD, Nathan C (2015) Rapid,
semiquantitative assay to discriminate among
compounds with activity against replicating or
nonreplicating Mycobacterium tuberculosis.
Antimicrob
Agents
Chemother
59
(10):6521–6538. https://doi.org/10.1128/
AAC.00803-15
12. Ballell L, Bates RH, Young RJ, AlvarezGomez D, Alvarez-Ruiz E, Barroso V,
Blanco D, Crespo B, Escribano J,
Gonzalez R, Lozano S, Huss S, SantosVillarejo A, Martin-Plaza JJ, Mendoza A,
Rebollo-Lopez MJ, Remuinan-Blanco M,
Lavandera JL, Perez-Herran E, Gamo-Benito
FJ, Garcia-Bustos JF, Barros D, Castro JP,
Cammack N (2013) Fueling open-source
drug discovery: 177 small-molecule leads
against tuberculosis. Chem Med Chem 8
(2):313–321.
https://doi.org/10.1002/
cmdc.201200428
13. Pethe K, Sequeira PC, Agarwalla S, Rhee K,
Kuhen K, Phong WY, Patel V, Beer D, Walker
JR, Duraiswamy J, Jiricek J, Keller TH,
Chatterjee A, Tan MP, Ujjini M, Rao SP,
Camacho L, Bifani P, Mak PA, Ma I, Barnes
SW, Chen Z, Plouffe D, Thayalan P, Ng SH,
Au M, Lee BH, Tan BH, Ravindran S,
Nanjundappa M, Lin X, Goh A, Lakshminarayana SB, Shoen C, Cynamon M, Kreiswirth B,
Dartois V, Peters EC, Glynne R, Brenner S,
Dick T (2010) A chemical genetic screen in
Mycobacterium tuberculosis identifies carbonsource-dependent growth inhibitors devoid of
in vivo efficacy. Nat Commun 1:57. https://
doi.org/10.1038/ncomms1060
14. Manjunatha UH, Smith PW (2015) Perspective: challenges and opportunities in TB drug
discovery from phenotypic screening. Bioorg
Med Chem 23(16):5087–5097. https://doi.
org/10.1016/j.bmc.2014.12.031
15. Ioerger TR, O’Malley T, Liao R, Guinn KM,
Hickey MJ, Mohaideen N, Murphy KC, Boshoff HI, Mizrahi V, Rubin EJ, Sassetti CM,
Barry CE 3rd, Sherman DR, Parish T, Sacchettini JC (2013) Identification of new drug targets
and
resistance
mechanisms
in
Mycobacterium tuberculosis. PLoS One 8(9):
e75245. https://doi.org/10.1371/journal.
pone.0075245
16. Stuehr DJ, Marletta MA (1985) Mammalian
nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide. Proc Natl Acad
Sci U S A 82(22):7738–7742. https://doi.
org/10.1073/pnas.82.22.7738
17. Blanco-Ruano D, Roberts DM, Gonzalez-DelRio R, Alvarez D, Rebollo MJ, Perez-Herran E,
Mendoza A (2015) Antimicrobial susceptibility
testing for Mycobacterium sp. Methods Mol
Biol 1285:257–268. https://doi.org/10.
1007/978-1-4939-2450-9_15
18. Cho S, Lee HS, Franzblau S (2015) Microplate
alamar blue assay (MABA) and low oxygen
recovery assay (LORA) for Mycobacterium
tuberculosis.
Methods
Mol
Biol
1285:281–292.
https://doi.org/10.1007/
978-1-4939-2450-9_17
19. Domenech P, Reed MB (2009) Rapid and
spontaneous loss of phthiocerol dimycocerosate (PDIM) from Mycobacterium tuberculosis
grown in vitro: implications for virulence studies. Microbiology 155(Pt 11):3532–3543.
https://doi.org/10.1099/mic.0.029199-0
20. Slayden RA, Barry CE 3rd (2001) Analysis of
the lipids of Mycobacterium tuberculosis.
Methods Mol Med 54:229–245. https://doi.
org/10.1385/1-59259-147-7:229
MIC Determination for Mycobacterium Tuberculosis
609