Acknowledgments
Funding for this work was provided in part by the Division of
Intramural Research, National Institute of Allergy and Infectious
Diseases, National Institutes of Health. The content of this publication does not necessarily reflect the views or policies of the
Department of Health and Human Services, nor does the mention
of trade names, commercial products, or organizations imply
endorsement by the U.S. Government.
References
1. VanderVen BC, Fahey RJ, Lee W, Liu Y, Abramovitch RB, Memmott C, Crowe AM, Eltis
LD, Perola E, Deininger DD, Wang T, Locher
CP, Russell DG (2015) Novel inhibitors of
cholesterol degradation in Mycobacterium
tuberculosis reveal how the bacterium’s metabolism is constrained by the intracellular environment. PLoS Pathog 11(2):e1004679.
https://doi.org/10.1371/journal.ppat.
1004679
2. Stanley SA, Barczak AK, Silvis MR, Luo SS,
Sogi K, Vokes M, Bray MA, Carpenter AE,
Moore CB, Siddiqi N, Rubin EJ, Hung DT
(2014) Identification of host-targeted small
molecules that restrict intracellular Mycobacterium tuberculosis growth. PLoS Pathog 10(2):
e1003946. https://doi.org/10.1371/journal.
ppat.1003946
3. Christophe T, Ewann F, Jeon HK, Cechetto J,
Brodin P (2010) High-content imaging of
Mycobacterium tuberculosis-infected macrophages: an in vitro model for tuberculosis
drug discovery. Future Med Chem 2
(8):1283–1293. https://doi.org/10.4155/
fmc.10.223
4. Darby CM, Ingolfsson HI, Jiang X, Shen C,
Sun M, Zhao N, Burns K, Liu G, Ehrt S, Warren JD, Andersen OS, Brickner SJ, Nathan C
(2013) Whole cell screen for inhibitors of pH
homeostasis in Mycobacterium tuberculosis.
PLoS One 8(7):e68942. https://doi.org/10.
1371/journal.pone.0068942
5. Mak PA, Rao SP, Ping Tan M, Lin X, Chyba J,
Tay J, Ng SH, Tan BH, Cherian J,
Duraiswamy J, Bifani P, Lim V, Lee BH, Ling
Ma N, Beer D, Thayalan P, Kuhen K,
Chatterjee A, Supek F, Glynne R, Zheng J,
Boshoff HI, Barry CE 3rd, Dick T, Pethe K,
Camacho LR (2012) A high-throughput
screen to identify inhibitors of ATP homeostasis in non-replicating Mycobacterium tuberculosis. ACS Chem Biol 7(7):1190–1197.
https://doi.org/10.1021/cb2004884
6. Wang F, Sambandan D, Halder R, Wang J, Batt
SM, Weinrick B, Ahmad I, Yang P, Zhang Y,
Kim J, Hassani M, Huszar S, Trefzer C, Ma Z,
Kaneko T, Mdluli KE, Franzblau S, Chatterjee
AK, Johnsson K, Mikusova K, Besra GS,
Futterer K, Robbins SH, Barnes SW, Walker
JR, Jacobs WR Jr, Schultz PG (2013) Identification of a small molecule with activity against
drug-resistant and persistent tuberculosis. Proc
Natl Acad Sci U S A 110(27):E2510–E2517.
https://doi.org/10.1073/pnas.1309171110
7. Grant SS, Kawate T, Nag PP, Silvis MR,
Gordon K, Stanley SA, Kazyanskaya E,
Nietupski R, Golas A, Fitzgerald M, Cho S,
Franzblau SG, Hung DT (2013) Identification
of novel inhibitors of nonreplicating Mycobacterium tuberculosis using a carbon starvation
model. ACS Chem Biol 8(10):2224–2234.
https://doi.org/10.1021/cb4004817
8. Ananthan S, Faaleolea ER, Goldman RC,
Hobrath JV, Kwong CD, Laughon BE, Maddry JA, Mehta A, Rasmussen L, Reynolds RC,
Secrist JA 3rd, Shindo N, Showe DN, Sosa MI,
Suling WJ, White EL (2009) High-throughput
screening for inhibitors of Mycobacterium
tuberculosis H37Rv. Tuberculosis (Edinb) 89
(5):334–353.
https://doi.org/10.1016/j.
tube.2009.05.008
9. Maddry JA, Ananthan S, Goldman RC,
Hobrath JV, Kwong CD, Maddox C,
Rasmussen L, Reynolds RC, Secrist JA 3rd,
Sosa MI, White EL, Zhang W (2009) Antituberculosis activity of the molecular libraries
screening center network library. Tuberculosis
(Edinb) 89(5):354–363. https://doi.org/10.
1016/j.tube.2009.07.006
10. Early JV, Casey A, Martinez-Grau MA, Gonzalez Valcarcel IC, Vieth M, Ollinger J, Bailey
MA, Alling T, Files M, Ovechkina Y, Parish T
(2016) Oxadiazoles have butyrate-specific conditional activity against Mycobacterium tuberculosis. Antimicrob Agents Chemother 60
608
Qinglan Wang and Helena I. M. Boshoff
Funding for this work was provided in part by the Division of
Intramural Research, National Institute of Allergy and Infectious
Diseases, National Institutes of Health. The content of this publication does not necessarily reflect the views or policies of the
Department of Health and Human Services, nor does the mention
of trade names, commercial products, or organizations imply
endorsement by the U.S. Government.
References
1. VanderVen BC, Fahey RJ, Lee W, Liu Y, Abramovitch RB, Memmott C, Crowe AM, Eltis
LD, Perola E, Deininger DD, Wang T, Locher
CP, Russell DG (2015) Novel inhibitors of
cholesterol degradation in Mycobacterium
tuberculosis reveal how the bacterium’s metabolism is constrained by the intracellular environment. PLoS Pathog 11(2):e1004679.
https://doi.org/10.1371/journal.ppat.
1004679
2. Stanley SA, Barczak AK, Silvis MR, Luo SS,
Sogi K, Vokes M, Bray MA, Carpenter AE,
Moore CB, Siddiqi N, Rubin EJ, Hung DT
(2014) Identification of host-targeted small
molecules that restrict intracellular Mycobacterium tuberculosis growth. PLoS Pathog 10(2):
e1003946. https://doi.org/10.1371/journal.
ppat.1003946
3. Christophe T, Ewann F, Jeon HK, Cechetto J,
Brodin P (2010) High-content imaging of
Mycobacterium tuberculosis-infected macrophages: an in vitro model for tuberculosis
drug discovery. Future Med Chem 2
(8):1283–1293. https://doi.org/10.4155/
fmc.10.223
4. Darby CM, Ingolfsson HI, Jiang X, Shen C,
Sun M, Zhao N, Burns K, Liu G, Ehrt S, Warren JD, Andersen OS, Brickner SJ, Nathan C
(2013) Whole cell screen for inhibitors of pH
homeostasis in Mycobacterium tuberculosis.
PLoS One 8(7):e68942. https://doi.org/10.
1371/journal.pone.0068942
5. Mak PA, Rao SP, Ping Tan M, Lin X, Chyba J,
Tay J, Ng SH, Tan BH, Cherian J,
Duraiswamy J, Bifani P, Lim V, Lee BH, Ling
Ma N, Beer D, Thayalan P, Kuhen K,
Chatterjee A, Supek F, Glynne R, Zheng J,
Boshoff HI, Barry CE 3rd, Dick T, Pethe K,
Camacho LR (2012) A high-throughput
screen to identify inhibitors of ATP homeostasis in non-replicating Mycobacterium tuberculosis. ACS Chem Biol 7(7):1190–1197.
https://doi.org/10.1021/cb2004884
6. Wang F, Sambandan D, Halder R, Wang J, Batt
SM, Weinrick B, Ahmad I, Yang P, Zhang Y,
Kim J, Hassani M, Huszar S, Trefzer C, Ma Z,
Kaneko T, Mdluli KE, Franzblau S, Chatterjee
AK, Johnsson K, Mikusova K, Besra GS,
Futterer K, Robbins SH, Barnes SW, Walker
JR, Jacobs WR Jr, Schultz PG (2013) Identification of a small molecule with activity against
drug-resistant and persistent tuberculosis. Proc
Natl Acad Sci U S A 110(27):E2510–E2517.
https://doi.org/10.1073/pnas.1309171110
7. Grant SS, Kawate T, Nag PP, Silvis MR,
Gordon K, Stanley SA, Kazyanskaya E,
Nietupski R, Golas A, Fitzgerald M, Cho S,
Franzblau SG, Hung DT (2013) Identification
of novel inhibitors of nonreplicating Mycobacterium tuberculosis using a carbon starvation
model. ACS Chem Biol 8(10):2224–2234.
https://doi.org/10.1021/cb4004817
8. Ananthan S, Faaleolea ER, Goldman RC,
Hobrath JV, Kwong CD, Laughon BE, Maddry JA, Mehta A, Rasmussen L, Reynolds RC,
Secrist JA 3rd, Shindo N, Showe DN, Sosa MI,
Suling WJ, White EL (2009) High-throughput
screening for inhibitors of Mycobacterium
tuberculosis H37Rv. Tuberculosis (Edinb) 89
(5):334–353.
https://doi.org/10.1016/j.
tube.2009.05.008
9. Maddry JA, Ananthan S, Goldman RC,
Hobrath JV, Kwong CD, Maddox C,
Rasmussen L, Reynolds RC, Secrist JA 3rd,
Sosa MI, White EL, Zhang W (2009) Antituberculosis activity of the molecular libraries
screening center network library. Tuberculosis
(Edinb) 89(5):354–363. https://doi.org/10.
1016/j.tube.2009.07.006
10. Early JV, Casey A, Martinez-Grau MA, Gonzalez Valcarcel IC, Vieth M, Ollinger J, Bailey
MA, Alling T, Files M, Ovechkina Y, Parish T
(2016) Oxadiazoles have butyrate-specific conditional activity against Mycobacterium tuberculosis. Antimicrob Agents Chemother 60
608
Qinglan Wang and Helena I. M. Boshoff