27. Remove the spent reagents container and discard the contents
in accordance with applicable standards. As you remove the
container, place your other hand underneath for support.
28. Slide the empty spent reagents container into the buffer compartment until it stops. An audible click indicates that the
container is in position.
29. Remove the used buffer cartridge from the upper
compartment.
30. Slide a new buffer cartridge into the buffer compartment until
it stops. An audible click indicates that the cartridge is in
position, the buffer cartridge ID appears on the screen, and
the sensor is checked.
31. Close the buffer compartment door, and select Next.
32. Remove the used reagent cartridge from the reagent compartment. Dispose of unused contents in accordance with applicable standards. Note: To facilitate safe disposal of unused
reagent, the reservoir in position 6 is removable.
33. Slide the reagent cartridge into the reagent compartment until
the cartridge stops and then close the reagent
compartment door.
34. Select Load. The software moves the cartridge into position
automatically (~30 s), the reagent cartridge ID appears on the
screen, and the sensors are checked.
35. Select Next.
36. After you have removed the used reagent cartridge from the
instrument, remove the protective rubber cover over the slot
next to position #6.
37. Press down on the clear plastic tab and push toward the left to
eject the reservoir.
38. Dispose of the reservoir in accordance with applicable
standards.
39. Review Automated Check. The software performs an automated check of the system. During the check, the following
indicators appear on the screen:
(a) Gray check mark—The check has not been performed yet.
(b) Progress icon —The check is in progress.
(c) Green check mark—The check passed.
(d) Red—The check did not pass. For any items that do not
pass, an action is required before you can proceed. See
Resolve Automatic Check Errors on page 34 of the Illumina NextSeq 500 System Guide (Document
#15046563v06).
To stop an automated check that is in progress, select
the “X” icon in the lower-right corner. To restart the
check, select the Circle Arrow icon. The check resumes
502
Shuyi Ma et al.
in accordance with applicable standards. As you remove the
container, place your other hand underneath for support.
28. Slide the empty spent reagents container into the buffer compartment until it stops. An audible click indicates that the
container is in position.
29. Remove the used buffer cartridge from the upper
compartment.
30. Slide a new buffer cartridge into the buffer compartment until
it stops. An audible click indicates that the cartridge is in
position, the buffer cartridge ID appears on the screen, and
the sensor is checked.
31. Close the buffer compartment door, and select Next.
32. Remove the used reagent cartridge from the reagent compartment. Dispose of unused contents in accordance with applicable standards. Note: To facilitate safe disposal of unused
reagent, the reservoir in position 6 is removable.
33. Slide the reagent cartridge into the reagent compartment until
the cartridge stops and then close the reagent
compartment door.
34. Select Load. The software moves the cartridge into position
automatically (~30 s), the reagent cartridge ID appears on the
screen, and the sensors are checked.
35. Select Next.
36. After you have removed the used reagent cartridge from the
instrument, remove the protective rubber cover over the slot
next to position #6.
37. Press down on the clear plastic tab and push toward the left to
eject the reservoir.
38. Dispose of the reservoir in accordance with applicable
standards.
39. Review Automated Check. The software performs an automated check of the system. During the check, the following
indicators appear on the screen:
(a) Gray check mark—The check has not been performed yet.
(b) Progress icon —The check is in progress.
(c) Green check mark—The check passed.
(d) Red—The check did not pass. For any items that do not
pass, an action is required before you can proceed. See
Resolve Automatic Check Errors on page 34 of the Illumina NextSeq 500 System Guide (Document
#15046563v06).
To stop an automated check that is in progress, select
the “X” icon in the lower-right corner. To restart the
check, select the Circle Arrow icon. The check resumes
502
Shuyi Ma et al.