In addition, accumulation and efflux assays can be used to
quantify and compare EtBr transport activity between mycobacterial strains (e.g., clinical isolates or mutants impaired in efflux or
permeability). For example, Fig. 4 (adapted from Ref. 25) presents
efflux of EtBr in two M. bovis BCG strains: M. bovis BCG (wildtype) and BCG-INH
R (an BCG derivative resistant to isoniazid by
the activity of efflux pumps). The BCG-INH
R strain showed an
increased efflux of EtBr (evidenced by a faster decrease in
Fig. 3 Efflux of EtBr by M. bovis BCG. Efflux takes place at 37
C in the presence
of glucose and is inhibited by TZ (adapted from Ref. 25). EI efflux inhibitor, TZ
thioridazine
Fig. 4 Efflux of EtBr by wild-type M. bovis BCG and BCG-INH
R
(isoniazid
resistance is induced by overexpression of efflux pump genes) (adapted from
Ref. 25)
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Liliana Rodrigues et al.
quantify and compare EtBr transport activity between mycobacterial strains (e.g., clinical isolates or mutants impaired in efflux or
permeability). For example, Fig. 4 (adapted from Ref. 25) presents
efflux of EtBr in two M. bovis BCG strains: M. bovis BCG (wildtype) and BCG-INH
R (an BCG derivative resistant to isoniazid by
the activity of efflux pumps). The BCG-INH
R strain showed an
increased efflux of EtBr (evidenced by a faster decrease in
Fig. 3 Efflux of EtBr by M. bovis BCG. Efflux takes place at 37
C in the presence
of glucose and is inhibited by TZ (adapted from Ref. 25). EI efflux inhibitor, TZ
thioridazine
Fig. 4 Efflux of EtBr by wild-type M. bovis BCG and BCG-INH
R
(isoniazid
resistance is induced by overexpression of efflux pump genes) (adapted from
Ref. 25)
234
Liliana Rodrigues et al.