glucose, efflux pumps are able to transport EtBr out and so fluorescence decreases quickly with time; and (2) in the presence of an
efflux inhibitor (such as TZ or VP), efflux is abolished and there is
no decrease in fluorescence.
Fig. 1 Accumulation of EtBr by M. bovis BCG
Fig. 2 Effect of efflux inhibitors on the accumulation of EtBr by M. bovis BCG.
EtBr was used at a concentration of 0.125 μg/mL and efflux inhibitors CPZ, TZ,
and VP at 5, 2.5, and 80 μg/mL, respectively (¼ MIC—Ref. 25) to not compromise cellular viability. CPZ chlorpromazine, EI efflux inhibitor, TZ thioridazine, VP
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Efflux and Permeability
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efflux inhibitor (such as TZ or VP), efflux is abolished and there is
no decrease in fluorescence.
Fig. 1 Accumulation of EtBr by M. bovis BCG
Fig. 2 Effect of efflux inhibitors on the accumulation of EtBr by M. bovis BCG.
EtBr was used at a concentration of 0.125 μg/mL and efflux inhibitors CPZ, TZ,
and VP at 5, 2.5, and 80 μg/mL, respectively (¼ MIC—Ref. 25) to not compromise cellular viability. CPZ chlorpromazine, EI efflux inhibitor, TZ thioridazine, VP
verapamil
Efflux and Permeability
233