2.3 Preparation
of Semipermeable
Membranes
1. Spectrum Spectra/Por 6 Dialysis Tubing, 25 kD MWCO clear.
2. 500-mL and 1-L beakers.
3. Magnetic stirrer.
4. Stir bar.
5. Glass sheet, 40 Â 25 cm.
6. Ruler.
7. Dark fine-pointed marker.
8. Precision Wipes (Kimtech Science).
9. 70% Ethanol.
10. Methanol.
11. Vacuum chamber (Nalgene).
12. Vacuum pump.
13. Pattern 36-angled broad-tip tweezer with large paddle style
tips (120 mm), stainless steel.
14. Whatman Filter paper.
15. Microscope glass slides.
16. Binder clips.
2.4 Microfluidic
Mask Design
and Manufacturing
1. Software to design microelectromechanical systems, such as
LayoutEditor (https://layouteditor.com) (see Notes 3 and 4).
2. Plastic mask (Fig. 1a) (see Notes 5 and 6).
3. Glass mask, 5 Â 5 in.
4. Ruler.
5. Surgical scalpel blade.
6. Magic tape (Scotch).
2.5 Silicon Wafer
Mold Fabrication
1. Plastic mask secured on a 5 Â 5 in. glass mask.
2. Precision wafer handling tweezers.
3. 100-mm-diameter silicon wafer.
4. Nitrogen gas blow gun.
5. SU8 2150 photoresist (MicroChem) (see Note 7).
6. Spin coater (SUSS MicroTec).
7. Hot plate.
8. MA/BA6 mask aligner (SUSS MicroTec).
9. Isopropyl alcohol.
10. Propylene glycol methyl ether acetate (PGMEA).
11. Trichloro (1H,1H,2H,2H-perfluorooctyl) silane (PFOCTS).
12. Vacuum chamber (Nalgene).
13. Vacuum pump.
208
Giulia Manina and Neeraj Dhar
of Semipermeable
Membranes
1. Spectrum Spectra/Por 6 Dialysis Tubing, 25 kD MWCO clear.
2. 500-mL and 1-L beakers.
3. Magnetic stirrer.
4. Stir bar.
5. Glass sheet, 40 Â 25 cm.
6. Ruler.
7. Dark fine-pointed marker.
8. Precision Wipes (Kimtech Science).
9. 70% Ethanol.
10. Methanol.
11. Vacuum chamber (Nalgene).
12. Vacuum pump.
13. Pattern 36-angled broad-tip tweezer with large paddle style
tips (120 mm), stainless steel.
14. Whatman Filter paper.
15. Microscope glass slides.
16. Binder clips.
2.4 Microfluidic
Mask Design
and Manufacturing
1. Software to design microelectromechanical systems, such as
LayoutEditor (https://layouteditor.com) (see Notes 3 and 4).
2. Plastic mask (Fig. 1a) (see Notes 5 and 6).
3. Glass mask, 5 Â 5 in.
4. Ruler.
5. Surgical scalpel blade.
6. Magic tape (Scotch).
2.5 Silicon Wafer
Mold Fabrication
1. Plastic mask secured on a 5 Â 5 in. glass mask.
2. Precision wafer handling tweezers.
3. 100-mm-diameter silicon wafer.
4. Nitrogen gas blow gun.
5. SU8 2150 photoresist (MicroChem) (see Note 7).
6. Spin coater (SUSS MicroTec).
7. Hot plate.
8. MA/BA6 mask aligner (SUSS MicroTec).
9. Isopropyl alcohol.
10. Propylene glycol methyl ether acetate (PGMEA).
11. Trichloro (1H,1H,2H,2H-perfluorooctyl) silane (PFOCTS).
12. Vacuum chamber (Nalgene).
13. Vacuum pump.
208
Giulia Manina and Neeraj Dhar