discovery using Mycobacteria-infected amoebae identifies anti-infectives and new molecular
targets. Sci Rep 8. https://doi.org/10.1038/
s41598-018-22228-6
19. Loomis WF (2014) Cell signaling during
development of Dictyostelium. Dev Biol 391
(1):1–16. https://doi.org/10.1016/j.ydbio.
2014.04.001
20. Bloomfield G, Tanaka Y, Skelton J, Ivens A,
Kay RR (2008) Widespread duplications in
the genomes of laboratory stocks of Dictyostelium discoideum. Genome Biol 9(4):R75.
https://doi.org/10.1186/gb-2008-9-4-r75
21. Watts DJ, Ashworth JM (1970) Growth of
myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J 119(2):171–174. https://doi.org/10.
1042/bj1190171
22. Lemieux MG, Janzen D, Hwang R, Roldan J,
Jarchum I, Knecht DA (2014) Visualization of
the actin cytoskeleton: different F-actin-binding probes tell different stories. Cytoskeleton
71(3):157–169. https://doi.org/10.1002/
cm.21160
23. Chan K, Knaak T, Satkamp L, Humbert O,
Falkow S, Ramakrishnan L (2002) Complex
pattern of Mycobacterium marinum gene
expression during long-term granulomatous
infection. Proc Natl Acad Sci U S A 99
(6):3920–3925. https://doi.org/10.1073/
pnas.002024599
24. Solomon JM, Leung GS, Isberg RR (2003)
Intracellular replication of Mycobacterium
marinum within Dictyostelium discoideum:
efficient replication in the absence of host coronin. Infect Immun 71(6):3578–3586.
https://doi.org/10.1128/IAI.71.6.35783586.2003
25. Toniolo C, Delince ´ M, McKinney JD (2018)
Chapter 11 – A microfluidic cell-trapping
device to study dynamic host-microbe interactions at the single-cell level. In: Piel M,
Fletcher D, Doh J (eds) Methods in cell biology, vol 147. Microfluidics in cell biology part
B: microfluidics in single cells. Academic Press,
Cambridge, pp 199–213
26. Fey P, Kowal AS, Gaudet P, Pilcher KE,
Chisholm RL (2007) Protocols for growth
and development of Dictyostelium discoideum. Nat Protoc 2(6):1307–1316. https://
doi.org/10.1038/nprot.2007.178
27. Manders EM, Stap J, Brakenhoff GJ, van
Driel R, Aten JA (1992) Dynamics of threedimensional replication patterns during the
S-phase, analysed by double labelling of DNA
and confocal microscopy. J Cell Sci 103
(Pt 3):857–862
28. Bolte S, Cordelie `res FP (2006) A guided tour
into subcellular colocalization analysis in light
microscopy. J Microsc 224(3):213–232.
https://doi.org/10.1111/j.1365-2818.2006.
01706.x
Monitoring Infection Dynamics of M. marinum in D. Discoideum
203
targets. Sci Rep 8. https://doi.org/10.1038/
s41598-018-22228-6
19. Loomis WF (2014) Cell signaling during
development of Dictyostelium. Dev Biol 391
(1):1–16. https://doi.org/10.1016/j.ydbio.
2014.04.001
20. Bloomfield G, Tanaka Y, Skelton J, Ivens A,
Kay RR (2008) Widespread duplications in
the genomes of laboratory stocks of Dictyostelium discoideum. Genome Biol 9(4):R75.
https://doi.org/10.1186/gb-2008-9-4-r75
21. Watts DJ, Ashworth JM (1970) Growth of
myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J 119(2):171–174. https://doi.org/10.
1042/bj1190171
22. Lemieux MG, Janzen D, Hwang R, Roldan J,
Jarchum I, Knecht DA (2014) Visualization of
the actin cytoskeleton: different F-actin-binding probes tell different stories. Cytoskeleton
71(3):157–169. https://doi.org/10.1002/
cm.21160
23. Chan K, Knaak T, Satkamp L, Humbert O,
Falkow S, Ramakrishnan L (2002) Complex
pattern of Mycobacterium marinum gene
expression during long-term granulomatous
infection. Proc Natl Acad Sci U S A 99
(6):3920–3925. https://doi.org/10.1073/
pnas.002024599
24. Solomon JM, Leung GS, Isberg RR (2003)
Intracellular replication of Mycobacterium
marinum within Dictyostelium discoideum:
efficient replication in the absence of host coronin. Infect Immun 71(6):3578–3586.
https://doi.org/10.1128/IAI.71.6.35783586.2003
25. Toniolo C, Delince ´ M, McKinney JD (2018)
Chapter 11 – A microfluidic cell-trapping
device to study dynamic host-microbe interactions at the single-cell level. In: Piel M,
Fletcher D, Doh J (eds) Methods in cell biology, vol 147. Microfluidics in cell biology part
B: microfluidics in single cells. Academic Press,
Cambridge, pp 199–213
26. Fey P, Kowal AS, Gaudet P, Pilcher KE,
Chisholm RL (2007) Protocols for growth
and development of Dictyostelium discoideum. Nat Protoc 2(6):1307–1316. https://
doi.org/10.1038/nprot.2007.178
27. Manders EM, Stap J, Brakenhoff GJ, van
Driel R, Aten JA (1992) Dynamics of threedimensional replication patterns during the
S-phase, analysed by double labelling of DNA
and confocal microscopy. J Cell Sci 103
(Pt 3):857–862
28. Bolte S, Cordelie `res FP (2006) A guided tour
into subcellular colocalization analysis in light
microscopy. J Microsc 224(3):213–232.
https://doi.org/10.1111/j.1365-2818.2006.
01706.x
Monitoring Infection Dynamics of M. marinum in D. Discoideum
203