63
Large-Scale Production of BNC
By.this.process.the.production.of.3D.BNC.bodies.can.be.realized.using.the.
two-step.method.for.cultivation.of.G. hansenii.(Porto.et.al..2010).
(Semi)continuous generation of Flat BNC
Inspired.by.the.increasing.demand.for.a.commercial.production.process.for.
planar.BNC.material.for.medical.as.well.as.technical.applications.and.supported.by.the.approaches.discussed.earlier,.Kralisch.et.al..(2010a).designed.
a.new,.efficient.process.for.the.(semi)continuous.cultivation.of.planar.BNC.
fleeces.and.foils..By.means.of.the.so-called.horizontal-lift.reactor.concept,.a.
continuous. tape. of. BNC. material. with. freely. selectable. length. and. adjustable.thickness.can.be.produced..The.planar.BNC.material.generated.has.the.
same. homogeneous,. undisturbed. 3D. network. structure. as. when. cultured.
under. static. conditions.. This. beneficial. combination. of. the. advantages. of.
continuous.processing.and.nearly.static.cultivation.results.in.smooth.extraction.of.the.BNC,.which.is.produced.in.this.apparatus.and.has.reached.the.
size.requested..It.is.lifted.up.and.transported.out.of.the.culture.apparatus.
in.a.continuous.manner.using.an.extractor.device.at.the.opposite.side.of.the.
apparatus.without.disrupting.the.surface.of.the.culture.medium.and.without.disturbing.the.BNC.sheet,.which.is.under.cultivation..At.the.front.side.of.
the.apparatus,.new.BNC.starts.to.grow.again.in.the.vacant.part.of.the.interface.between.the.air.and.culture.medium.
In. order. to. test. and. optimize. this. continuous. production. process. for.
. planar. BNC. fleeces. or. foils,. two. plants. (Schmidt. et. al.. 2010). based. on. this.
concept.were.constructed..First,.an.experimental.HoLiR.plant.was.designed.
as.a.plane.culture.apparatus.(V.=.20.l).made.from.stainless.steel..Two.inlet.
and.two.outlet.tubes,.arranged.at.the.front.and.the.back.of.the.apparatus,.
allow.for.continuous.dosage.and.regulation.of.the.culture.medium..The.cultivation.temperature.can.be.maintained.using.an.electrical.heating.pad..The.
whole.apparatus.is.enclosed.by.a.housing.and.filtered.air.is.passed.through.
this. housing. in. order. to. ensure. an. appropriate. oxygen. content. in. the. air..
After.an.initial.cultivation.phase,.the.BNC,.which.has.been.produced.in.this.
apparatus.and.has.reached.the.size.requested,.is.lifted.up.and.transported.
out. of. the. culture. apparatus. in. a. continuous. or. gradual. manner. using. an.
extractor.device..The.output,.still.not.cut.off.from.the.rest,.is.immediately.
treated.with.a.bacteria-deadening.and.cleaning.solution.in.order.to.inhibit.
ongoing.growth.of.the.BNC..This.is.followed.by.a.cleaning.procedure.using.
a. (semi)continuous. system. of. rollers,. belt. conveyors,. and. baths. filled. with.
cleaning.agents.
After.a.successful.proof.of.concept.by.means.of.the.experimental.plant,.a.
pilot.plant.was.constructed.by.transferal.of.the.plant.characteristics.described.
earlier..For.this,.the.dimensions.of.the.culture.apparatus.were.scaled.up.to.a.
volume.of.180.l.
Large-Scale Production of BNC
By.this.process.the.production.of.3D.BNC.bodies.can.be.realized.using.the.
two-step.method.for.cultivation.of.G. hansenii.(Porto.et.al..2010).
(Semi)continuous generation of Flat BNC
Inspired.by.the.increasing.demand.for.a.commercial.production.process.for.
planar.BNC.material.for.medical.as.well.as.technical.applications.and.supported.by.the.approaches.discussed.earlier,.Kralisch.et.al..(2010a).designed.
a.new,.efficient.process.for.the.(semi)continuous.cultivation.of.planar.BNC.
fleeces.and.foils..By.means.of.the.so-called.horizontal-lift.reactor.concept,.a.
continuous. tape. of. BNC. material. with. freely. selectable. length. and. adjustable.thickness.can.be.produced..The.planar.BNC.material.generated.has.the.
same. homogeneous,. undisturbed. 3D. network. structure. as. when. cultured.
under. static. conditions.. This. beneficial. combination. of. the. advantages. of.
continuous.processing.and.nearly.static.cultivation.results.in.smooth.extraction.of.the.BNC,.which.is.produced.in.this.apparatus.and.has.reached.the.
size.requested..It.is.lifted.up.and.transported.out.of.the.culture.apparatus.
in.a.continuous.manner.using.an.extractor.device.at.the.opposite.side.of.the.
apparatus.without.disrupting.the.surface.of.the.culture.medium.and.without.disturbing.the.BNC.sheet,.which.is.under.cultivation..At.the.front.side.of.
the.apparatus,.new.BNC.starts.to.grow.again.in.the.vacant.part.of.the.interface.between.the.air.and.culture.medium.
In. order. to. test. and. optimize. this. continuous. production. process. for.
. planar. BNC. fleeces. or. foils,. two. plants. (Schmidt. et. al.. 2010). based. on. this.
concept.were.constructed..First,.an.experimental.HoLiR.plant.was.designed.
as.a.plane.culture.apparatus.(V.=.20.l).made.from.stainless.steel..Two.inlet.
and.two.outlet.tubes,.arranged.at.the.front.and.the.back.of.the.apparatus,.
allow.for.continuous.dosage.and.regulation.of.the.culture.medium..The.cultivation.temperature.can.be.maintained.using.an.electrical.heating.pad..The.
whole.apparatus.is.enclosed.by.a.housing.and.filtered.air.is.passed.through.
this. housing. in. order. to. ensure. an. appropriate. oxygen. content. in. the. air..
After.an.initial.cultivation.phase,.the.BNC,.which.has.been.produced.in.this.
apparatus.and.has.reached.the.size.requested,.is.lifted.up.and.transported.
out. of. the. culture. apparatus. in. a. continuous. or. gradual. manner. using. an.
extractor.device..The.output,.still.not.cut.off.from.the.rest,.is.immediately.
treated.with.a.bacteria-deadening.and.cleaning.solution.in.order.to.inhibit.
ongoing.growth.of.the.BNC..This.is.followed.by.a.cleaning.procedure.using.
a. (semi)continuous. system. of. rollers,. belt. conveyors,. and. baths. filled. with.
cleaning.agents.
After.a.successful.proof.of.concept.by.means.of.the.experimental.plant,.a.
pilot.plant.was.constructed.by.transferal.of.the.plant.characteristics.described.
earlier..For.this,.the.dimensions.of.the.culture.apparatus.were.scaled.up.to.a.
volume.of.180.l.