Figure 3.10
Figure 3.11
(a)
(b)
62
Bacterial Cellulose
Nutrient
Medium
Figure 3.10
Rotating.disk.and.linear.conveyors.reactors.(adopted.from.U.S..15,955,326).
Figure 3.11
Biosynthesis.of.BC.in.the.RDF..(a).Cultivation.in.RDF.(b).BC.attached.to.the.discs.after.7.days.
of.culture.or.with.rollers.(Krystynowicz.et.al..2002).
is. temporarily. dipped. in. the. liquid. medium. during. cultivation.. The. BNC.
attaches.and.accumulates.on.the.rough.surface.of.the.rollers.or.discs,.which.
rotate.along.the.long.axis.and.are.temporarily.dipped.in.the.liquid.nutrient.
medium..In.their.study,.they.used.two.different.types.of.horizontal.fermenters.equipped.with.plastic.discs.or.rollers.(see.Figure 3.11)..They.found.many.
similarities. between. the. time. course. of. the. BNC. synthesis. in. the. rotating.
disk.fermenter.and.in.a.typical.stationary.culture..Differences.were.related.
to.the.membrane.thickness,.which.was.slightly.greater.in.the.case.of.culture.
in.the.rotating.disk.fermenter,.whereas.other.parameters,.such.as.cell.numbers,.pH,.and.glucose.utilization,.were.similar.
They. found. that. “at. a. higher. rotation. speed,. cellulose. membranes. that.
accumulated.on.the.surface.of.the.discs.were.much.thicker,.despite.the.fact.
that.the.total.mass.of.synthesized.cellulose.was.lower”.as.a.result.of.a.greater.
degree.of.cellulose.hydration..The.liquid.layer.on.the.surface.of.the.disk.is.
dependent.on.disk.diameter.and.the.material.used,.as.well.as.on.the.depth.
of.the.disk.immersion.in.the.culture.medium.
Also. BNC. biomaterial. in. several. forms. suggested. for. tissue. engineering.
has. been. developed. in. a. combination. of. static. and. agitated. cultivations..
Figure 3.11
(a)
(b)
62
Bacterial Cellulose
Nutrient
Medium
Figure 3.10
Rotating.disk.and.linear.conveyors.reactors.(adopted.from.U.S..15,955,326).
Figure 3.11
Biosynthesis.of.BC.in.the.RDF..(a).Cultivation.in.RDF.(b).BC.attached.to.the.discs.after.7.days.
of.culture.or.with.rollers.(Krystynowicz.et.al..2002).
is. temporarily. dipped. in. the. liquid. medium. during. cultivation.. The. BNC.
attaches.and.accumulates.on.the.rough.surface.of.the.rollers.or.discs,.which.
rotate.along.the.long.axis.and.are.temporarily.dipped.in.the.liquid.nutrient.
medium..In.their.study,.they.used.two.different.types.of.horizontal.fermenters.equipped.with.plastic.discs.or.rollers.(see.Figure 3.11)..They.found.many.
similarities. between. the. time. course. of. the. BNC. synthesis. in. the. rotating.
disk.fermenter.and.in.a.typical.stationary.culture..Differences.were.related.
to.the.membrane.thickness,.which.was.slightly.greater.in.the.case.of.culture.
in.the.rotating.disk.fermenter,.whereas.other.parameters,.such.as.cell.numbers,.pH,.and.glucose.utilization,.were.similar.
They. found. that. “at. a. higher. rotation. speed,. cellulose. membranes. that.
accumulated.on.the.surface.of.the.discs.were.much.thicker,.despite.the.fact.
that.the.total.mass.of.synthesized.cellulose.was.lower”.as.a.result.of.a.greater.
degree.of.cellulose.hydration..The.liquid.layer.on.the.surface.of.the.disk.is.
dependent.on.disk.diameter.and.the.material.used,.as.well.as.on.the.depth.
of.the.disk.immersion.in.the.culture.medium.
Also. BNC. biomaterial. in. several. forms. suggested. for. tissue. engineering.
has. been. developed. in. a. combination. of. static. and. agitated. cultivations..