2
Effect of Cultivation Conditions on the
Structure and Morphological Properties of BNC
Biomaterials with a Focus on Vascular Grafts
Paul Gatenholm, 1 Kim Höglund, 1 Sara Johannesson, 1 Maja Puchades, 1
Christian Brackmann, 2 Annika Enejder, 2 and Lisbeth Olsson 3
1 Department of Chemical and Biological Engineering,
Biosynthetic Blood Vessel Laboratory, Biopolymer Technology,
Chalmers University of Technology, Gothenburg, Sweden
2 Department of Chemical and Biological Engineering,
Life Sciences, Molecular Microscopy, and Biotechnology,
Chalmers University of Technology, Gothenburg, Sweden
3 Department of Chemical and Biological Engineering, Industrial Biotechnology,
Chalmers University of Technology, Gothenburg, Sweden
CONTENTS
Introduction............................................................................................................ 20

Bacterial.Cellulose.Production:.A.Part.of.the.Carbon.Metabolism.in.

G. xylinus.................................................................................................................22

Factors.Involved.during.Nanocellulose.Network.Formation.by G. xylinus.....24

Effect.of.Growth.Medium.Components.on.Bacterial.Cellulose.

Production.in.G. xylinus........................................................................................ 26

Lactate................................................................................................................. 27

Ethanol................................................................................................................ 28

Carbon.Substrate............................................................................................... 28

Corn.Steep.Liquor............................................................................................. 28

Genetic.Modifications.of.G. xylinus for.Enhanced Production.of.
Bacterial.Cellulose. ................................................................................................. 29

Transfection.of.G. xylinus with.the.pBla-VHb-122.Plasmid........................ 29

Spontaneous.Mutation.in.G. xylinus Can.Lead.to.a.Loss.of.the.

Ability.to.Produce.Cellulose. ........................................................................... 30

Production.of.Artificial.Blood.Vessels by.G. xylinus in.Bioreactors................ 31

Tube.Bioreactor.................................................................................................. 32

Cellulose.Production.Yield.and.Cellulose.Content...................................... 35

Cellulose.Content.............................................................................................. 36

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